All Stories

  1. Graphene oxide coatings enhance fluorescence signals in a lateral flow immunoassay for the detection of UCH-L1, a marker for trauma brain injury
  2. Manufacturing of non-viral protein nanocages for biotechnological and biomedical applications
  3. Purification of scaffolds for DNA origami
  4. Maximizing mRNA vaccine production
  5. New options for the design of lateral flow immunoassays
  6. Cellulose-based antimicrobial materials
  7. Fishing DNA scaffolds with magnetic beads
  8. Analysis of DNA minicircle production
  9. Biofunctionalization of cellulose hydrogels
  10. Minicircle‐based expression of vascular endothelial growth factor (VEGF) in mesenchymal stromal cells (MSC) from diverse human tissues
  11. Manufacturing mRNA vaccines
  12. Manufacturing bacteriophages for antimicrobial therapy
  13. Rapid detection of cardiac troponin I
  14. Purification of minicircle biopharmaceuticals
  15. G Protein-Coupled Receptor Screening Assays
  16. GPCR Microfluidic Assays
  17. Protease Biosensors
  18. Fluorescent gold nanorods
  19. Enzymes as Downstream Processing Aids
  20. Fractionation of Silver Nanotriangles
  21. Colorimetric detection of DNA on cellulose microparticles
  22. Plasmid copy number in Lactococcus
  23. Multimodal chromatography of DNA minicircles
  24. Extreme Enhancement of Single-Molecule Fluorescence from Porphyrins Induced by Gold Nanodimer Antennas
  25. Gold Nanotriangles as Selective Catalysts for Cyclohexanol and Cyclohexanone Production
  26. Engineering of human mesenchymal stem/stromal cells (MSC) with VEGF-encoding minicircles for angiogenic ex vivo gene therapy
  27. Purification of minicircles by nicking and chromatography
  28. Analysis of metallic nanoparticles embedded in thin film semiconductors for optoelectronic applications
  29. Re-engineering bacteria for recombinant protein production
  30. Colorimetric Detection of D-dimer in a Paper-based Device
  31. A biomolecular recognition approach for the functionalization of cellulose with gold nanoparticles
  32. Purification of plasmid DNA by multimodal chromatography
  33. Type A carbohydrate-binding modules and S. thermophila CBM64 structure
  34. Fluorescence correlation spectroscopy study of the complexation of DNA hybrids, IgG antibody, and a chimeric protein of IgG-binding ZZ domains fused with a carbohydrate binding module
  35. Enhanced-Fluorescence of a Dye on DNA- assembled Gold Nano-Dimers Discriminated by Lifetime Correlation Spectroscopy
  36. Use of DNA Stabilizers to Extend Plasmid Biological Activity
  37. Separation of plasmid DNA topoisomers by multimodal chromatography
  38. Improvement of DNA minicircle production by optimization of the secondary structure of the 5′-UTR of ParA resolvase
  39. Purification of minicircles
  40. Dynamics of Droplets of Biological Fluids on Smooth Superhydrophobic Surfaces under Electrostatic Actuation
  41. Towards effective non-viral gene delivery vector
  42. G Protein-Coupled Receptor Screening Assays
  43. G protein-Coupled Receptors: An Overview of Signaling Mechanisms and Screening Assays
  44. Preparation of liposome membrane adsorbers and testing for plasmid purification
  45. Use of ImageJ to Recover Information from Individual Cells in a G Protein-Coupled Receptor Assay
  46. Plasmid Biopharmaceuticals
  47. Thin-film amorphous silicon photodiodes with integrated fluorescent filters for monitoring live-cell G-protein coupled receptors (GPCR)
  48. Biomimetic replicas of English weed leaves
  49. Plasmid production with engineered E. coli strain
  50. Biomimetic materials for construction
  51. In situNIR spectroscopy monitoring of plasmid production processes: effect of producing strain, medium composition and the cultivation strategy
  52. On the dual effect of glucose during production of pBAD/AraC-based minicircles
  53. Engineering of Escherichia coli strains for plasmid biopharmaceutical production: Scale-up challenges
  54. Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters
  55. DNA tests on paper
  56. Development of a phenyl membrane chromatography-based process yielding pharmaceutical grade plasmid deoxyribonucleic acid for mammalian cells transfection
  57. Detection of GPCR activation
  58. Enhancement of DNA Vaccine Efficacy by Intracellular Targeting Strategies
  59. Impact of plasmid DNA quality on biological activity
  60. Impact of plasmid size on the purification of model plasmid DNA vaccines by phenyl membrane adsorbers
  61. Streaming currents in microfluidics with integrated polarizable electrodes
  62. Metabolic viability of Escherichia coli trapped by dielectrophoresis in microfluidics
  63. Detection of ochratoxin A in wine and beer by chemiluminescence-based ELISA in microfluidics with integrated photodiodes
  64. Towards the miniaturization of GPCR-based live-cell screening assays
  65. Validation and scale‐up of plasmid DNA purification by phenyl‐boronic acid chromatography
  66. Identification of IS2 transposition in pVAX1-based plasmid, a common vector for DNA vaccine development
  67. Preferential transposition of the mobile element IS2 into AT- and GC-skew polarity switches
  68. Improved bacterial production of DNA
  69. Integrated detection of intrinsic fluorophores in live microbial cells using an array of thin film amorphous silicon photodetectors
  70. Development of a recombinant fusion protein based on the dynein light chain LC8 for non-viral gene delivery
  71. Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition
  72. Toward Therapeutic Plasmids Purification by Hydrophobic Interaction Membrane Chromatography
  73. Streaming current measurements in micro and nanofluidic channels for label-free multiplexed genomics diagnostics
  74. Liposome Derived Membrane Adsorber for Purification of Nucleic Acids
  75. Integrated On-chip Photodetection of Intracellular Calcium in Response to the Activation of G-protein Coupled Receptors
  76. Plasmid purification by phenyl-boronate chromatography
  77. Electrical detection of DNA immobilization and hybridization by streaming current measurements in microchannels
  78. Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing
  79. Integration of thin film amorphous silicon photodetector with lab-on-chip for monitoring protein fluorescence in solution and in live microbial cells
  80. DNA biopharmaceuticals
  81. Membrane chromatography for therapeutic DNA production: Adsorption membranes development
  82. Towards a high-throughput drug discovery platform for the screening of GPCR targets in cells
  83. Mutation detection in plasmid‐based biopharmaceuticals
  84. Heterogeneous immunoassays in microfluidic format using fluorescence detection with integrated amorphous silicon photodiodes
  85. Microspot-based ELISA in microfluidics: chemiluminescence and colorimetry detection using integrated thin-film hydrogenated amorphous silicon photodiodes
  86. Trypanosoma brucei: Immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis
  87. Amorphous Silicon Photosensors for Detection of Intrinsic Cell Fluorophores
  88. Purification of plasmid DNA from Escherichia coli ferments using anion‐exchange membrane and hydrophobic chromatography
  89. Lab-on-a-Chip Ochratoxin A Detection Using Competitive ELISA in Microfluidics with Integrated Photodiode Signal Acquisition
  90. Optimization of DNA Hybridization on Aminopropyl-Controlled Pore-Glass Particles: Detection of Non-Labeled Targets by PicoGreen Staining
  91. Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography
  92. Characterisation of hydrogenated silicon–carbon alloy filters with different carbon composition for on-chip fluorescence detection of biomolecules
  93. Erratum to: Comparative Analysis of Antigen-Targeting Sequences Used in DNA Vaccines
  94. Patterned functionalization layer for sub-μL DNA solid-phase immobilization and hybridization
  95. Erratum to: Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events
  96. Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events
  97. Clearance of host cell impurities from plasmid-containing lysates by boronate adsorption
  98. Hydrophobic interaction membrane chromatography for plasmid DNA purification: Design and optimization
  99. Production and Purification of Adenovirus Vectors for Gene Therapy
  100. DNA vaccines: a rational design against parasitic diseases
  101. Spectral selectivity constraints in fluo- rescence detection of biomolecules using amorphous silicon based detectors
  102. Chromatographic Separation of Plasmid DNA Using Macroporous Beads
  103. Comparative Analysis of Antigen-Targeting Sequences Used in DNA Vaccines
  104. Evidence for the in vivo expression of a distant downstream gene under the control of ColE1 replication origin
  105. Comparison of amorphous silicon photodiodes and photoconductors for detection of quantum dot biomolecular tags
  106. Quantitation of non-amplified genomic DNA by bead-based hybridization and template mediated extension coupled to alkaline phosphatase signal amplification
  107. Chemiluminescent Detection of Horseradish Peroxidase Using an Integrated Amorphous Silicon Thin-Film Photosensor
  108. Structural instability of plasmid biopharmaceuticals: challenges and implications
  109. Deletion formation mutations in plasmid expression vectors are unfavored by runaway amplification conditions and differentially selected under kanamycin stress
  110. Ionic Conductivity Measurements in a SiO2 Nanochannel with PDMS Interconnects
  111. DNA biosensors: towards a microparticle based-platform for genomic DNA detection
  112. Stabilization of naked and condensed plasmid DNA against degradation induced by ultrasounds and high‐shear vortices
  113. Application of central composite design for DNA hybridization onto magnetic microparticles
  114. Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis
  115. Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention
  116. Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus
  117. The role of probe–probe interactions on the hybridization of double-stranded DNA targets onto DNA-modified magnetic microparticles
  118. Recovery and partial purification of penicillin G acylase from E. coli homogenate and B. megaterium culture medium using an expanded bed adsorption column
  119. Detection of fluorescently labeled biomolecules immobilized on a detachable substrate using an integrated amorphous silicon photodetector
  120. Evaluation of the Effect of Non-B DNA Structures on Plasmid Integrity Via Accelerated Stability Studies
  121. Alternatives for the intermediate recovery of plasmid DNA: Performance, economic viability and environmental impact
  122. Plasmid purification by hydrophobic interaction chromatography using sodium citrate in the mobile phase
  123. Purification of plasmid DNA with aqueous two phase systems of PEG 600 and sodium citrate/ammonium sulfate
  124. Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification
  125. Enzymatic Biosensors with Integrated Thin Film a-Si:H Photodiodes
  126. Miniaturization of Immunoassays Using Optical Detection with Integrated Amorphous Silicon Photodiodes
  127. Purification of plasmid DNA using tangential flow filtration and tandem anion-exchange membrane chromatography
  128. The effect of the shape of single, sub-ms voltage pulses on the rates of surface immobilization and hybridization of DNA
  129. Detection of DNA and proteins using amorphous silicon ion-sensitive thin-film field effect transistors
  130. Colorimetric detection of molecular recognition reactions with an enzyme biolabel using a thin-film amorphous silicon photodiode on a glass substrate
  131. Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography
  132. Developing a vaccine for African trypanosomiasis: only wishful thinking or a definite possibility?
  133. Recombination frequency in plasmid DNA containing direct repeats—predictive correlation with repeat and intervening sequence length
  134. Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA
  135. Amorphous Silicon Thin-Film Transistors Gated Through an Electrolyte Solution
  136. Fluorescence detection of DNA using an amorphous silicon p-i-n photodiode
  137. High Frequency Plasmid Recombination Mediated by 28 bp Direct Repeats
  138. Chemiluminescent bead-based hybridization assay for the detection of genomic DNA from E. coli in purified plasmid samples
  139. Detection of molecular tags with an integrated amorphous silicon photodetector for biological applications
  140. Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography
  141. Prediction of diffusion coefficients of plasmids
  142. Electric Field Assisted DNA Surface Reactions on the Sub-ms Timescale
  143. Electrical and Chemical Control of Surfaces for DNA Immobilization and Hybridization
  144. Fluorescence Detection of DNA Hybridization Using an Integrated Thin-Film Amorphous Silicon n-i-p Photodiode
  145. Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography
  146. Electrochemical Detection of a Dengue-related Oligonucleotide Sequence Using Ferrocenium as a Hybridization Indicator
  147. Development of a candidate DNA vaccine against Maedi-Visna virus
  148. Stabilization of plasmid DNA against mechanical damage
  149. Integration of plasmid DNA purification and molecular therapy agent preparation using aqueous two-phase systems
  150. Histidine-agarose chromatography: Effect of plasmid DNA structure and topology on retention
  151. Preparation of plasmid DNA polyplexes from alkaline lysates by a two-step aqueous two-phase extraction process
  152. Time-course determination of plasmid content in eukaryotic and prokaryotic cells using Real-Time PCR
  153. On the stability of plasmid DNA vectors during cell culture and purification
  154. Dynamic binding capacity of plasmid DNA in histidine–agarose chromatography
  155. Kinetics and stability of a chromobacterium viscosum lipase in reversed micellar and aqueous media
  156. pH sensitive photoconductor based on poly(para-phenylene-vinylene)
  157. The impact of polyadenylation signals on plasmid nuclease‐resistance and transgene expression
  158. Extraction of Dengue 2 Plasmid DNA Vaccine (pD2) from Cell Lysates by Aqueous Two-Phase Systems
  159. Detection of Chemiluminescence Using an Amorphous Silicon Photodiode
  160. On-Chip Control of DNA Immobilization and Hybridization with Nanosecond Electric Field Pulses
  161. Chemical synthesis and crystallization of the dipeptide AcPheIleNH2 in TTAB/heptane/octanol reversed micelles
  162. On-chip Detection of Chemiluminescent Biomolecules Using an Integrated Thin Film Silicon Photodiode
  163. Plasmid DNA
  164. Selective purification of supercoiled plasmid DNA from clarified cell lysates with a single histidine–agarose chromatography step
  165. Production and Purification of Adenovirus Vectors for Gene Therapy
  166. Optimization of Isopropanol and Ammonium Sulfate Precipitation Steps in the Purification of Plasmid DNA
  167. Thin-film silicon MEMS DNA sensors
  168. Label-free electronic detection of biomolecules using a-Si:H field-effect devices
  169. a-Si:H Electrolyte-Gate Thin Film Devices for Biological Applications
  170. Performance of Thin-film a-Si:H Microresonators in Dissipative Media
  171. Optimization of the primary recovery of human interferon α2b from Escherichia coli inclusion bodies
  172. Single base mismatch detection by microsecond voltage pulses
  173. Ethanol biosensors based on alcohol oxidase
  174. Electric-field assisted immobilization and hybridization of DNA oligomers on thin-film microchips
  175. Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography
  176. Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine–agarose support
  177. Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography
  178. Chromatography of plasmid DNA
  179. Purification of Plasmid DNA Vectors Produced in Escherichia coli for Gene Therapy and DNA Vaccination Applications
  180. Trends in dengue diagnosis
  181. Translational Features of Human Alpha 2b Interferon Production in Escherichia coli
  182. Immobilization and hybridization by single sub-millisecond electric field pulses, for pixel-addressed DNA microarrays
  183. Design of flowsheets for the recovery and purification of plasmids for gene therapy and DNA vaccination
  184. The role of polyadenylation signal secondary structures on the resistance of plasmid vectors to nucleases
  185. Optoelectronic detection of DNA molecules using an amorphous silicon photodetector
  186. Thermal and operational stabilities of Hansenula polymorpha alcohol oxidase
  187. Application of factorial design to the study of an alcoholysis transformation promoted by cutinase immobilized on NaY zeolite and Accurel PA6
  188. Covalent immobilization of DNA and hybridization on microchips by microsecond electric field pulses
  189. Comparison of real-time polymerase chain reaction and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products
  190. Electric-field-pulse-assisted covalent immobilization of DNA in the nanosecond time scale
  191. Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B
  192. Assessment of purity and quantification of plasmid DNA in process solutions using high-performance hydrophobic interaction chromatography
  193. Membrane-Assisted Extractive Bioconversions
  194. Horseradish peroxidase
  195. Use of free and immobilized Pseudomonas putida cells for the reduction of a thiophene derivative in organic media
  196. Stability of a recombinant cutinase immobilized on zeolites
  197. Isolation of plasmid DNA from cell lysates by aqueous two-phase systems
  198. Industrial biochromatography
  199. Microbial Stereoselective Oxidation of 2-methyl-1,3-propanediol to (R)-β-hydroxyisobutyric Acid in Aqueous/organic Biphasic Systems
  200. Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B
  201. Stability of free and immobilised peroxidase in aqueous–organic solvents mixtures
  202. A Multiphasic Hollow Fiber Reactor for the Whole-Cell Bioconversion of 2-Methyl-1,3-propanediol to (R)-β-Hydroxyisobutyric Acid
  203. Plasmid Manufacturing – An Overview
  205. The Second International Conference on Protein Stabilisation, 9–12 April 2000, Lisbon
  206. Production, purification and analysis of an experimental DNA vaccine against rabies
  207. Purification of plasmids for gene therapy and DNA vaccination
  208. Kinetics and modelling of an alcoholysis reaction catalyzed by cutinase immobilized on NaY zeolite
  209. Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications
  210. Analysis of a Taylor–Poiseuille vortex flow reactor — II: reactor modeling and performance assessment using glucose-fructose isomerization as test reaction
  211. The effect of stirring and seeding on the AcPheLeuNH2 synthesis and crystallization in a reversed micellar system
  212. Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography
  213. Studies on the Batch Adsorption of Plasmid DNA onto Anion-Exchange Chromatographic Supports
  214. Appendix 1. Essential Guides for Isolation/Purification of Nucleic Acids
  215. Organic media selection for the extraction of ?-hydroxyisobutyric acid produced by microbial biotransformation
  216. Kinetic and Stability Studies of Penicillin Acylase in Reversed Micelles
  217. Biotransformations in two-liquid-phase systems
  218. Stability and stabilisation of penicillin acylase
  219. Separation and Analysis of Plasmid Denatured Forms Using Hydrophobic Interaction Chromatography
  220. Development of Process Flow Sheets for the Purification of Supercoiled Plasmids for Gene Therapy Applications
  221. Enantioselective oxidation of (RS)-2-phenyl-1-propanol to (S)-2-phenylpropanoic acid with Gluconobacter oxydans: simplex optimization of the biotransformation
  222. Continuous production and simultaneous precipitation of a dipeptide in a reversed micellar membrane reactor
  223. Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: problems and bottlenecks
  224. Analysis and use of endogenous nuclease activities inEscherichia coli lysates during the primary isolation of plasmids for gene therapy
  225. Monitoring of Process Streams in the Large-scale Production and Purification of Plasmid DNA for Gene Therapy Applications
  226. Purification of supercoiled plasmid DNA using chromatographic processes
  227. Analysis of a Taylor–Poiseuille vortex flow reactor—I
  228. Zeolites as supports for an enzymatic alcoholysis reaction
  229. Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography
  230. Biotransformation in organic media by enzymes and whole cells
  231. Quantitative structure activity relationships in the synthesis of AcXYNH2 dipeptides by α-chymotrypsin in reversed micelles
  232. Continuous production of isovaleraldehyde through extractive bioconversion in a hollow-fiber membrane bioreactor
  233. A theoretical analogy between multistage ultrafiltration and size-exclusion chromatography
  234. Effect of the immobilization support on the hydrolytic activity of a cutinase from Fusarium solani pisi
  235. Optimal design of a number of membrane reactors in series using Michaelis-Menten one-substrate reversible kinetics
  236. Zeolites as supports for enzymatic hydrolysis reactions. Comparative study of several zeolites
  237. Phospholipase A2‐catalyzed hydrolysis of lecithin in a continuous reversed‐micellar membrane bioreactor
  238. Solubility Studies and Synthesis of Acpheleunh2in Reversed Micellar Systems
  239. Reversed Micellar Membrane Bioreactor
  240. Modeling of Membrane Bioreactors
  241. Optimal design ofnmembrane reactors in series using michaelis-menten kinetics
  242. Hydrolysis of lecithin by phospholipase A2 in mixed reversed micelles of lecithin and sodium dioctyl sulphosuccinate
  243. Concentration of BSA using a superabsorbent polymer: process evaluation
  244. Adsorption studies for the separation ofl-tryptophan froml-serine and indole in a bioconversion medium
  245. Adsorption studies for the separation of
  246. Optimal Design Of N Cstrs in Series Using Michaelis-Menten One-Substrate Reversible Reaction
  247. Dipeptide synthesis and separation in a reversed micellar membrane reactor
  248. Enzymatic membrane bioreactors and their applications
  249. Synthesis of AcPheLeuNH2 by α‐chymotrypsin in TTAB reversed micelles: Application of response surface methodology to the optimization of the system
  250. Continuous lipolysis in a reversed micellar membrane bioreactor
  251. Modeling lipolysis in a reversed micellar system: Part II—membrane reactor
  252. Modeling lipolysis in a reversed micellar system: Part I. Conventional batch reactor
  253. Protein assay in reversed micelle solutions
  254. Temperature, pH and Media Influence on Lipase Stability
  255. Colorimetric assays for the determination of dioctyl sodium sulphosuccinate and glycerol in reversed micelles
  256. Batch and Continuous Lipolysis/Product Separation in a Reversed Micellar Membrane Bioreactor
  258. DNA Vaccines