All Stories

  1. Rejection-Focused Precision Medicine in Kidney Transplantation: Biology, Biomarkers, and Artificial Intelligence

    Article • Life, April 2026, MDPI AG

    Professor Cecília R.C. Calado

  2. Machine Learning-Assisted FTIR Spectroscopy Analysis of Kidney Preservation Fluids for Delayed Graft Function Risk Stratification

    Article • Journal of Clinical Medicine, April 2026, MDPI AG

    Professor Cecília R.C. Calado

  3. FT-MIR Spectroscopy Coupled with Machine Learning for the Identification of Antimicrobial Spectral Markers in Marine Drug Discovery

    Article • January 2026, Elsevier

    Professor Cecília R.C. Calado

  4. Comparison of Mid and Near-Infrared Spectroscopy to Predict Creatinine, Urea and Albumin in Serum Samples as Biomarkers of Renal Function

    Article • Biosensors, December 2025, MDPI AG

    Professor Cecília R.C. Calado

  5. Rapid FTIR Spectral Fingerprinting of Kidney Allograft Perfusion Fluids Distinguishes DCD from DBD Donors: A Pilot Machine Learning Study

    Article • Metabolites, October 2025, MDPI AG

    Professor Cecília R.C. Calado

  6. Multiplex Targeted Proteomic Analysis of Cytokine Ratios for ICU Mortality in Severe COVID-19

    Article • Proteomes, August 2025, MDPI AG

    Professor Cecília R.C. Calado

  7. Analyzing Heart Rate Variability for COVID-19 ICU Mortality Prediction Using Continuous Signal Processing Techniques

    Article • Journal of Clinical Medicine, July 2025, MDPI AG

    Professor Cecília R.C. Calado

  8. Predictive Models of Patient Severity in Intensive Care Units Based on Serum Cytokine Profiles: Advancing Rapid Analysis

    Article • Applied Sciences, April 2025, MDPI AG

    Professor Cecília R.C. Calado

  9. Cytokine-Based Insights into Bloodstream Infections and Bacterial Gram Typing in ICU COVID-19 Patients

    Article • Metabolites, March 2025, MDPI AG

    Professor Cecília R.C. Calado

  10. Integration of FTIR Spectroscopy and Machine Learning for Kidney Allograft Rejection: A Complementary Diagnostic Tool

    Article • Journal of Clinical Medicine, January 2025, MDPI AG

    Professor Cecília R.C. Calado

  11. Chitosan Nanoparticles for Enhanced Immune Response and Delivery of Multi-Epitope Helicobacter pylori Vaccines in a BALB/c Mouse Model

    Article • Pharmaceutics, January 2025, MDPI AG

    Professor Cecília R.C. Calado

  12. Comparison of two metabolomics-platforms to discover biomarkers in critically ill patients from serum analysis

    Article • Computers in Biology and Medicine, January 2025, Elsevier

    Professor Cecília R.C. Calado

  13. Early Mortality Prediction in Intensive Care Unit Patients Based on Serum Metabolomic Fingerprint

    Article • International Journal of Molecular Sciences, December 2024, MDPI AG

    Professor Cecília R.C. Calado

  14. Analysis of six consecutive waves of ICU-admitted COVID-19 patients: key findings and insights from a Portuguese population

    Article • GeroScience, November 2024, Springer Science + Business Media

    Professor Cecília R.C. Calado

  15. Impact of neutrophil‐activating protein conservation on diagnostic tests and vaccine design

    Article • Journal of Chemical Technology & Biotechnology, September 2024, Wiley

    Professor Cecília R.C. Calado

  16. Discovery of Delirium Biomarkers through Minimally Invasive Serum Molecular Fingerprinting

    Article • Metabolites, May 2024, MDPI AG

    Professor Cecília R.C. Calado

  17. A new methodology for a rapid and high‐throughput comparison of molecular profiles and biological activity of phytoextracts

    Article • Biotechnology and Bioengineering, May 2024, Wiley

    Professor Cecília R.C. Calado

  18. Bridging the gap between target-based and phenotypic-based drug discovery

    Article • Expert Opinion on Drug Discovery, May 2024, Taylor & Francis

    Professor Cecília R.C. Calado

  19. Enhancing Bioactive Compound Classification through the Synergy of Fourier-Transform Infrared Spectroscopy and Advanced Machine Learning Methods

    Article • Antibiotics, May 2024, MDPI AG

    Professor Cecília R.C. Calado

  20. Simplifying Data Analysis in Biomedical Research: An Automated, User-Friendly Tool

    Article • Methods and Protocols, April 2024, MDPI AG

    Professor Cecília R.C. Calado

  21. Enhancing Bioactive Compound Classification through the Synergy of Fourier-Transform Infrared Spectroscopy and Advanced Machine Learning Methods

    Article • April 2024, MDPI AG

    Professor Cecília R.C. Calado

  22. Predicting Cellular Rejection of Renal Allograft Based on the Serum Proteomic Fingerprint

    Article • International Journal of Molecular Sciences, March 2024, MDPI AG

    Professor Cecília R.C. Calado, Miguel Bigotte Vieira

  23. Exosomes and microvesicles in kidney transplantation: the long road from trash to gold

    Article • Pathology, February 2024, Elsevier

    Professor Cecília R.C. Calado

  24. The Characteristics and Laboratory Findings of SARS-CoV-2 Infected Patients during the First Three COVID-19 Waves in Portugal—A Retrospective Single-Center Study

    Article • Medicina, December 2023, MDPI AG

    Professor Cecília R.C. Calado

  25. Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs

    Article • Mutation Research/Genetic Toxicology and Environmental Mutagenesis, October 2023, Elsevier

    Professor Cecília R.C. Calado

  26. A new methodology for a rapid and high-throughput comparison of molecular profiles and biological activity of phytoextracts

    Article • August 2023, Authorea, Inc.

    Professor Cecília R.C. Calado

  27. Alternative Serum Biomarkers of Bacteraemia for Intensive Care Unit Patients

    Article • June 2023, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  28. Comparison of Analytical Methods Of Serum Untargeted Metabolomics

    Article • June 2023, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  29. The Impact of the Serum Extraction Protocol on Metabolomic Profiling Using UPLC-MS/MS and FTIR Spectroscopy

    Article • ACS Omega, June 2023, American Chemical Society (ACS)

    Professor Cecília R.C. Calado

  30. Label-free discrimination of T and B lymphocyte activation based on vibrational spectroscopy – A machine learning approach

    Article • Vibrational Spectroscopy, May 2023, Elsevier

    Professor Cecília R.C. Calado

  31. Quality Monitoring of Biodiesel and Diesel/Biodiesel Blends: A Comparison between Benchtop FT-NIR versus a Portable Miniaturized NIR Spectroscopic Analysis

    Article • Processes, April 2023, MDPI AG

    Professor Cecília R.C. Calado

  32. Epigenetic and Drug Response Modulation of Epigalocaten-In-3-Gallate in Staphylococcus aureus with Divergent Resistance Phenotypes

    Article • Antibiotics, March 2023, MDPI AG

    Professor Cecília R.C. Calado, Professor Miguel Brito

  33. Plasma versus Serum Analysis by FTIR Spectroscopy to Capture the Human Physiological State

    Article • BioTech, December 2022, MDPI AG

    Professor Cecília R.C. Calado

  34. Antimicrobial evaluation of the Cynara cardunculus extract in Helicobacter pylori cells using mid-infrared spectroscopy and chemometric methods

    Article • Journal of Applied Microbiology, September 2022, Oxford University Press (OUP)

    Professor Cecília R.C. Calado

  35. Proteomics for Biomarker Discovery for Diagnosis and Prognosis of Kidney Transplantation Rejection

    Article • Proteomes, July 2022, MDPI AG

    Professor Cecília R.C. Calado

  36. Ovine Model as a Temporomandibular Disc Substitute: Characterisation and the Outcomes of Freezing Storage

    Article • May 2022, MDPI AG

    Professor Cecília R.C. Calado

  37. Antigenic and conserved peptides from diverse Helicobacter pylori antigens

    Article • Biotechnology Letters, March 2022, Springer Science + Business Media

    Professor Cecília R.C. Calado

  38. Infection Biomarkers Based on Metabolomics

    Article • Metabolites, January 2022, MDPI AG

    Professor Cecília R.C. Calado

  39. Development of a biocompatible hydrogel based on native temporomandibular joint extracellular matrix powder

    Article • World Review of Science Technology and Sustainable Development, January 2022, Inderscience Publishers

    Professor Cecília R.C. Calado

  40. Fast identification of off‐target liabilities in early antibiotic discovery with Fourier‐transform infrared spectroscopy

    Article • Biotechnology and Bioengineering, August 2021, Wiley

    Professor Cecília R.C. Calado

  41. A new method to predict genotoxic effects based on serum molecular profile

    Article • Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, July 2021, Elsevier

    Professor Cecília R.C. Calado

  42. Technologies for High-Throughput Identification of Antibiotic Mechanism of Action

    Article • Antibiotics, May 2021, MDPI AG

    Professor Cecília R.C. Calado

  43. VARIABILITY OF NEUTROPHIL-ACTIVATING PROTEIN AMONG HELICOBACTER PYLORI STRAINS

    Article • Romanian Archives of Microbiology and Immunology, March 2021, Institutul National de Cercetare Dezvoltare Medico-Militara "Cantacuzino"

    Professor Cecília R.C. Calado

  44. Comparison of partial least squares‐discriminant analysis and soft independent modeling of class analogy methods for classification of Saccharomyces cerevisiae cells based on mid‐infrared spectroscopy

    Article • Journal of Chemometrics, March 2021, Wiley

    Professor Cecília R.C. Calado

  45. Simultaneous elucidation of antibiotic mechanism of action and potency with high-throughput Fourier-transform infrared (FTIR) spectroscopy and machine learning

    Article • Applied Microbiology and Biotechnology, January 2021, Springer Science + Business Media

    Professor Cecília R.C. Calado

  46. Potential of FTIR-Spectroscopy for Drugs Screening against Helicobacter pylori

    Article • Antibiotics, December 2020, MDPI AG

    Professor Cecília R.C. Calado

  47. Discriminating B and T-lymphocyte from its molecular profile acquired in a label-free and high-throughput method

    Article • Vibrational Spectroscopy, November 2020, Elsevier

    Professor Cecília R.C. Calado

  48. FTIR spectroscopy applications for biomedicine

    Article • August 2020, Bentham Science Publishers

    Cecília Calado, Professor Cecília R.C. Calado

  49. A Simple, Label-Free, and High-Throughput Method to Evaluate the Epigallocatechin-3-Gallate Impact in Plasma Molecular Profile

    Article • High-Throughput, April 2020, MDPI AG

    Professor Cecília R.C. Calado

  50. Metabolic Fingerprinting with Fourier-Transform Infrared (FTIR) Spectroscopy: Towards a High-Throughput Screening Assay for Antibiotic Discovery and Mechanism-of-Action Elucidation

    Article • Metabolites, April 2020, MDPI AG

    Professor Cecília R.C. Calado

  51. A phenotypic screening bioassay forEscherichia colistress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis

    Article • Journal of Applied Microbiology, October 2019, Oxford University Press (OUP)

    Professor Cecília R.C. Calado

  52. Antibiotic Discovery: Where Have We Come from, Where Do We Go?

    Article • Antibiotics, April 2019, MDPI AG

    Professor Cecília R.C. Calado

  53. Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy

    Article • Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, March 2019, Elsevier

    Professor Cecília R.C. Calado

  54. Assessing the molecular fingerprint of T lymphocyte activation

    Article • February 2019, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  55. Spectral Biomarkers of Genotoxicity from Methanol Extracts of Blood

    Article • February 2019, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  56. Impact of the solvent extraction method on the plasma metabolome profile

    Article • February 2019, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  57. Classification of recombinant Saccharomyces cerevisiae cells using PLS-DA modelling based on MIR spectroscopy

    Article • February 2019, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  58. Effect of culturing human embryos in group on the composition of spent culture media

    Article • February 2019, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  59. Towards an automated statistical workflow for biomarker screening in Fourier-transform infrared spectroscopy

    Article • February 2019, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  60. Impact of the human mesenchymal stem cells donor on conditional medium composition

    Article • February 2019, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  61. Effect of consumption of green tea extracts on the plasma molecular signature

    Article • February 2019, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  62. Helicobacter pylori Persistence Throughout the Host Life as a High Risk of Peptic Ulcers, Gastric Cancer and MALT Lymphoma

    Article • January 2019, Avid Science

    Professor Cecília R.C. Calado

  63. Determination of Cell Abundances and Paralytic Shellfish Toxins in Cultures of the Dinoflagellate Gymnodinium catenatum by Fourier Transform Near Infrared Spectroscopy

    Article • Journal of Marine Science and Engineering, December 2018, MDPI AG

    Professor Cecília R.C. Calado

  64. Assessing plasmid bioprocess reproducibility and C-source uptake stage through multivariate analysis of offline and online data

    Article • Journal of Chemical Technology & Biotechnology, May 2018, Wiley

    Professor Cecília R.C. Calado

  65. Comparative analysis of different transformed Saccharomyces cerevisiae strains based on high-throughput Fourier transform infrared spectroscopy

    Article • Journal of Biotechnology, October 2017, Elsevier

    Professor Cecília R.C. Calado

  66. High-throughput FTIR-based bioprocess analysis of recombinant cyprosin production

    Article • Journal of Industrial Microbiology & Biotechnology, January 2017, Oxford University Press (OUP)

    Professor Cecília R.C. Calado

  67. Optimization of a bioassay to evaluate Escherichia coli stress responses

    Article • January 2017, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  68. Biopharmaceuticals process monitoring based on infrared spectroscopy according to Quality by Design

    Article • January 2017, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  69. High-throughput bioassay for mechanism of action determination of antibacterial drugs

    Article • January 2017, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  70. Mid-infrared spectroscopy: A groundbreaking tool for monitoring mammalian cells processes

    Article • January 2017, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  71. Does Nonlinear Modeling Play a Role in Plasmid Bioprocess Monitoring Using Fourier Transform Infrared Spectra?

    Article • Applied Spectroscopy, November 2016, SAGE Publications

    Professor Cecília R.C. Calado

  72. Metabolic profiling of recombinantEscherichia colicultivations based on high-throughput FT-MIR spectroscopic analysis

    Article • Biotechnology Progress, October 2016, Wiley

    Professor Cecília R.C. Calado

  73. Monitoring theex-vivoexpansion of human mesenchymal stem/stromal cells in xeno-free microcarrier-based reactor systems by MIR spectroscopy

    Article • Biotechnology Progress, February 2016, Wiley

    Professor Cecília R.C. Calado

  74. A comprehensive high-throughput FTIR spectroscopy-based method for evaluating the transfection event: estimating the transfection efficiency and extracting associated metabolic responses

    Article • Analytical and Bioanalytical Chemistry, September 2015, Springer Science + Business Media

    Professor Cecília R.C. Calado

  75. In Situ Near-Infrared (NIR) versus High-Throughput Mid-Infrared (MIR) Spectroscopy to Monitor Biopharmaceutical Production

    Article • Applied Spectroscopy, June 2015, SAGE Publications

    Professor Cecília R.C. Calado

  76. Molecular fingerprint of human gastric cell line infected by Helicobacter pylori

    Article • February 2015, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  77. Development of a high-throughput monitoring technique of bacteria photodynamic inactivation

    Article • February 2015, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  78. Process Analytical Technologies in Biopharmaceutical Process Development

    Article • Journal of Chemical Technology & Biotechnology, January 2015, Wiley

    Dr Anurag Singh Rathore, Professor Cecília R.C. Calado, Professor Krist V. Gernaey

  79. In situ near infrared spectroscopy monitoring of cyprosin production by recombinant Saccharomyces cerevisiae strains

    Article • Journal of Biotechnology, October 2014, Elsevier

    Professor Cecília R.C. Calado

  80. Kinetic modeling of plasmid bioproduction in Escherichia coli DH5α cultures over different carbon-source compositions

    Article • Journal of Biotechnology, September 2014, Elsevier

    Professor Cecília R.C. Calado

  81. In situNIR spectroscopy monitoring of plasmid production processes: effect of producing strain, medium composition and the cultivation strategy

    Article • Journal of Chemical Technology & Biotechnology, June 2014, Wiley

    Professor Duarte Miguel Prazeres, Professor Cecília R.C. Calado

  82. Real-time plasmid monitoring of batch and fed-batch Escherichia coli cultures by NIR spectroscopy

    Article • February 2013, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  83. High-throughput analysis of the plasmid bioproduction process in Escherichia coli by FTIR spectroscopy

    Article • Biotechnology and Bioengineering, April 2012, Wiley

    Professor Cecília R.C. Calado

  84. Development and characterization of a new plasmid delivery system based on chitosan–sodium deoxycholate nanoparticles

    Article • European Journal of Pharmaceutical Sciences, March 2012, Elsevier

    Professor Cecília R.C. Calado

  85. The importance of Helicobacter pylori's genetic variability for the construction of an efficient vaccine

    Article • February 2012, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  86. Modelling, monitoring and control of plasmid bioproduction in Escherichia coli cultures

    Article • February 2012, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  87. Protein and DNA nanoparticulate multiantigenic vaccines against H. pylori: In vivo evaluation

    Article • February 2012, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  88. A Nonionic Porphyrin as a Noninterfering DNA Antibacterial Agent

    Article • Photochemistry and Photobiology, September 2011, Wiley

    Antonio Rocha Gonsalves, Professor Cecília R.C. Calado

  89. Use of chemometrics in the selection of a Saccharomyces cerevisiae expression system for recombinant cyprosin B production

    Article • Biotechnology Letters, July 2011, Springer Science + Business Media

    Professor Cecília R.C. Calado

  90. Antigenic Diversity Among Portuguese Clinical Isolates of Helicobacter pylori

    Article • Helicobacter, March 2011, Wiley

    Professor Cecília R.C. Calado

  91. Biodegradable nanoparticles of alginate and chitosan as non-viral DNA oral delivery system

    Article • March 2011, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  92. Monitoring bacterial processes by Fourier transform infrared spectroscopy: Helicobacter pylori drug inactivation and plasmid bioproduction in recombinant Escherichia coli cultures

    Article • March 2011, Institute of Electrical & Electronics Engineers (IEEE)

    Professor Cecília R.C. Calado

  93. Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production

    Article • European Food Research and Technology, April 2010, Springer Science + Business Media

    Professor Cecília R.C. Calado

  94. A novel multifunctional DNA vaccine expresses conserved and immunogenic fragments of six Helicobacter pylori proteins

    Article • New Biotechnology, September 2009, Elsevier

    Professor Cecília R.C. Calado

  95. The carbon-source on the efficiency of plasmid production in high density cultures of Escherichia coli

    Article • New Biotechnology, September 2009, Elsevier

    Professor Cecília R.C. Calado

  96. Influence of tryptophan tags on the purification of cutinase, secreted by a recombinant Saccharomyces cerevisiae, using cationic expanded bed adsorption and hydrophobic interaction chromatography

    Article • Biotechnology Letters, March 2008, Springer Science + Business Media

    Professor Cecília R.C. Calado

  97. Prediction of retention time of cutinases tagged with hydrophobic peptides in hydrophobic interaction chromatography

    Article • Journal of Chromatography A, June 2007, Elsevier

    Professor Cecília R.C. Calado

  98. A flow injection analysis system for on‐line monitoring of cutinase activity at outlet of an expanded bed adsorption column almost in real time

    Article • Journal of Chemical Technology & Biotechnology, July 2006, Wiley

    Professor Cecília R.C. Calado

  99. Micro-analytical GO/HRP bioreactor for glucose determination and bioprocess monitoring

    Article • Biosensors and Bioelectronics, April 2005, Elsevier

    Professor Cecília R.C. Calado

  100. Integration of the production and the purification processes of cutinase secreted by a recombinant Saccharomyces cerevisiae SU50 strain

    Article • Journal of Biotechnology, April 2004, Elsevier

    Professor Cecília R.C. Calado

  101. Recombinant Saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways

    Article • Journal of Biotechnology, April 2004, Elsevier

    Professor Cecília R.C. Calado

  102. Pressure-tuning FTIR Spectroscopy: Applications to Biomedical Research and Diagnosis

    Article • January 2004, American Institute of Physics

    Professor Cecília R.C. Calado

  103. Towards a cost effective strategy for cutinase production by a recombinant Saccharomyces cerevisiae: strain physiological aspects

    Article • Applied Microbiology and Biotechnology, January 2003, Springer Science + Business Media

    Professor Cecília R.C. Calado

  104. Integration of production and aqueous two-phase systems extraction of extracellular cutinase fusion proteins

    Article • Journal of Biotechnology, January 2003, Elsevier

    Professor Cecília R.C. Calado

  105. Development of a Fed-Batch Cultivation Strategy for the Enhanced production and Secretion of Cutinase by a Recombinant Saccharomyces cerevisiae SU50 Strain

    Article • Journal of Bioscience and Bioengineering, January 2003, Elsevier

    Professor Cecília R.C. Calado

  106. Development of a Fed-Batch Cultivation Strategy for the Enhanced Production and Secretion of Cutinase by a Recombinant Saccharomyces cerevisiae SU50 Strain

    Article • Journal of Bioscience and Bioengineering, January 2003, Elsevier

    Professor Cecília R.C. Calado

  107. Effect of Saccharomyces cerevisiae fermentation conditions on expanded bed adsorption of heterologous cutinase

    Article • Journal of Chemical Technology & Biotechnology, October 2002, Wiley

    Professor Cecília R.C. Calado

  108. Optimisation of culture conditions and characterisation of cutinase produced by recombinant Saccharomyces cerevisiae

    Article • Enzyme and Microbial Technology, July 2002, Elsevier

    Professor Cecília R.C. Calado

  109. Production of wild-type and peptide fusion cutinases by recombinantSaccharomyces cerevisiae MM01 strains

    Article • Biotechnology and Bioengineering, April 2002, Wiley

    Professor Cecília R.C. Calado

  110. Effect of pre-fermentation on the production of cutinase by a recombinant Saccharomyces cerevisiae

    Article • Journal of Bioscience and Bioengineering, April 2002, Elsevier

    Professor Cecília R.C. Calado

  111. Article • Bioseparation, January 2001, Springer Science + Business Media

    Professor Cecília R.C. Calado