What is it about?
The paper describes the relevance of an engineered E. coli strain in the production of plasmid DNA using bench scale-bioreactors.
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Why is it important?
The new strain, which yielded 2100-2200 mg plasmid DNA per L in 14 L bioreactors, maybe usefull for the large scale production of DNA vaccines and plasmid-based gene therapy vectors.
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This page is a summary of: Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: Fermentation strategies and impact on downstream processing, Journal of Biotechnology, September 2014, Elsevier,
DOI: 10.1016/j.jbiotec.2014.06.008.
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