All Stories

  1. Differences in intrinsic cellular O-GlcNAcylation impact response to metabolic stress
  2. Differential substrate preferences IN ACTINOBACTERIAL protein O-MANNOSYLTRANSFERASES and alteration of protein-O-MANNOSYLATION by choice of secretion pathway
  3. PROTEIN-O-MANNOSYLATION BY NON-SEC/TAT SECRETION TRANSLOCONS IN ACTINOBACTERIA
  4. A high-throughput screening platform for enzymes active on mucin-type O-glycoproteins
  5. O-GlcNAc transferase modulates the cellular endocytosis machinery by controlling the formation of clathrin-coated pits
  6. Attenuation of Polysialic Acid Biosynthesis in Cells by the Small Molecule Inhibitor 8-Keto-sialic acid
  7. O-glycosylation and its role in therapeutic proteins
  8. A previously uncharacterized O-glycopeptidase from Akkermansia muciniphila requires the Tn-antigen for cleavage of the peptide bond
  9. Chitin-Active Lytic Polysaccharide Monooxygenases Are Rare in Cellulomonas Species
  10. O-GlcNAc transferase modulates formation of clathrin-coated pits
  11. Mammalian sialyltransferases allow efficient E. coli-based production of mucin-type O-glycoproteins but can also transfer Kdo.
  12. Quantifying Carbohydrate-Active Enzyme Activity with Glycoprotein Substrates Using Electrospray Ionization Mass Spectrometry and Center-of-Mass Monitoring
  13. Investigation of sequon engineering for improved O-glycosylation by the human polypeptide N-acetylgalactosaminyl transferase T2 isozyme and two orthologues
  14. Architecturally complex O -glycopeptidases are customized for mucin recognition and hydrolysis
  15. Engineering polysialic acid on Schwann cells using polysialyltransferase gene transfer or purified enzyme exposure for spinal cord injury transplantation
  16. Development of BODIPY labelled sialic acids as sialyltransferase substrates for direct detection of terminal galactose on N- and O-linked glycans
  17. Energetic adaptations: Metabolic control of endocytic membrane traffic
  18. Comparison of α2,6-Sialyltransferases for sialylation of therapeutic proteins
  19. Directed evolution of bacterial polysialyltransferases
  20. Genetics behind the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides in Campylobacter coli
  21. A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans
  22. Genetics behind the biosynthesis of nonulosonic acid containing lipooligosaccharides in Campylobacter coli:
  23. X-ray crystallographic structure of a bacterial polysialyltransferase provides insight into the biosynthesis of capsular polysialic acid
  24. Recognition of protein-linked glycans as a determinant of peptidase activity
  25. Proteomic Analysis of the Secretome of Cellulomonas fimi ATCC 484 and Cellulomonas flavigena ATCC 482
  26. Construction of a hybrid β-hexosaminidase subunit capable of forming stable homodimers that hydrolyze GM2 ganglioside in vivo
  27. Structure of human ST8SiaIII sialyltransferase provides insight into cell-surface polysialylation
  28. Sialyltransferases with enhanced legionaminic acid transferase activity for the preparation of analogs of sialoglycoconjugates
  29. Looking at beta-glucosidases in the genome of C. fimi
  30. A plate-based high-throughput activity assay for polysialyltransferase from Neisseria meningitidis
  31. Review of phosphocholine substituents on bacterial pathogen glycans: Synthesis, structures and interactions with host proteins
  32. Biochemical Characterization of a Polysialyltransferase from Mannheimia haemolytica A2 and Comparison to Other Bacterial Polysialyltransferases
  33. In Cellulo Examination of a Beta-Alpha Hybrid Construct of Beta-Hexosaminidase A Subunits, Reported to Interact with the GM2 Activator Protein and Hydrolyze GM2 Ganglioside
  34. Structure-based mutagenic analysis of mechanism and substrate specificity in mammalian glycosyltransferases: Porcine ST3Gal-I
  35. A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis
  36. Domain Organization of the Polymerizing Mannosyltransferases Involved in Synthesis of theEscherichia coliO8 and O9a Lipopolysaccharide O-antigens
  37. Biosynthesis of the Polymannose Lipopolysaccharide O-antigens fromEscherichia coliSerotypes O8 and O9a Requires a Unique Combination of Single- and Multiple-active Site Mannosyltransferases
  38. Enzymatic Engineering of Polysialic Acid on Cellsin Vitroandin VivoUsing a Purified Bacterial Polysialyltransferase
  39. Structures of Merkel Cell Polyomavirus VP1 Complexes Define a Sialic Acid Binding Site Required for Infection
  40. Helicobacter pylori β1,3-N-acetylglucosaminyltransferase for versatile synthesis of type 1 and type 2 poly-LacNAcs on N-linked, O-linked and I-antigen glycans
  41. Thiogalactopyranosides are Resistant to Hydrolysis by α-Galactosidases
  42. Small molecules containing hetero-bicyclic ring systems compete with UDP-Glc for binding to WaaG glycosyltransferase
  43. Characterization of α2,3- and α2,6-sialyltransferases from Helicobacter acinonychis
  44. Recognition of Sialylated Poly‐N‐acetyllactosamine Chains on N‐ and O‐Linked Glycans by Human and Avian Influenza A Virus Hemagglutinins
  45. Recognition of Sialylated Poly-N-acetyllactosamine Chains on N- and O-Linked Glycans by Human and Avian Influenza A Virus Hemagglutinins
  46. Identification and Characterization of a Lipopolysaccharide  ,2,3-Sialyltransferase from the Human Pathogen Helicobacter bizzozeronii
  47. Inhibition of Galactosyltransferases by a Novel Class of Donor Analogues
  48. Transition State Analysis of Vibrio cholerae Sialidase-Catalyzed Hydrolyses of Natural Substrate Analogues
  49. Genetics and molecular specificity of sialylation of Histophilus somni lipooligosaccharide (LOS) and the effect of LOS sialylation on Toll-like receptor-4 signaling
  50. Bacterial glycosyltransferases in molecular mimicry
  51. Structure and Mechanism of the Lipooligosaccharide Sialyltransferase fromNeisseria meningitidis
  52. Structural and Kinetic Analysis of Substrate Binding to the Sialyltransferase Cst-II fromCampylobacter jejuni
  53. Site-specific enzymatic polysialylation of therapeutic proteins using bacterial enzymes
  54. Enzymatic synthesis and properties of glycoconjugates with legionaminic acid as a replacement for neuraminic acid
  55. Fluorescence Activated Cell Sorting as a General Ultra-High-Throughput Screening Method for Directed Evolution of Glycosyltransferases
  56. 89. Demonstration of the in cellulo efficacy of pyrimethamine as a pharmacological chaperone for late onset Tay-Sachs Disease using a fluorescent GM2 ganglioside analogue
  57. A sensitive fluorescence-based assay for monitoring GM2 ganglioside hydrolysis in live patient cells and their lysates
  58. NMR Spectroscopic Characterization of the Sialyltransferase CstII from Campylobacter jejuni: Histidine 188 Is the General Base
  59. Structural insight into mammalian sialyltransferases
  60. Mechanistic Investigation of the Endo-α-N-acetylgalactosaminidase from Streptococcus pneumoniae R6
  61. Structural and Kinetic Characterizations of the Polysialic AcidO-Acetyltransferase OatWY fromNeisseria meningitidis
  62. A New Sialidase Mechanism
  63. Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni and Pasteurella multocida
  64. The Structural Basis for T-antigen Hydrolysis byStreptococcus pneumoniae
  65. Chemo-enzymatic synthesis of poly-N-acetyllactosamine (poly-LacNAc) structures and their characterization for CGL2-galectin-mediated binding of ECM glycoproteins to biomaterial surfaces
  66. A β‐1,4‐Galactosyltransferase from Helicobacter pylori is an Efficient and Versatile Biocatalyst Displaying a Novel Activity for Thioglycoside Synthesis
  67. Characterization of the  -2,8-polysialyltransferase from Neisseria meningitidis with synthetic acceptors, and the development of a self-priming polysialyltransferase fusion enzyme
  68. Variants of the β1,3-Galactosyltransferase CgtB from the Bacterium Campylobacter Jejuni have Distinct Acceptor Specificities
  69. Distinct Endocytic Mechanisms of CD22 (Siglec-2) and Siglec-F Reflect Roles in Cell Signaling and Innate Immunity
  70. Structural Analysis of the α-2,3-Sialyltransferase Cst-I from Campylobacter jejuni in Apo and Substrate-Analogue Bound Forms,
  71. Combination of UDP‐Glc(NAc) 4′‐Epimerase and Galactose Oxidase in a One‐Pot Synthesis of Biotinylated Nucleotide Sugars
  72. Mechanism of stabilization of Bacillus circulans xylanase upon the introduction of disulfide bonds
  73. Alternative donor substrates for inverting and retaining glycosyltransferases
  74. Identification of a Bifunctional Lipopolysaccharide Sialyltransferase inHaemophilus influenzae
  75. Using substrate engineering to harness enzymatic promiscuity and expand biological catalysis
  76. High-throughput screening methodology for the directed evolution of glycosyltransferases
  77. Chemical and Chemoenzymatic Synthesis of S‐Linked Ganglioside Analogues and Their Protein Conjugates for Use as Immunogens.
  78. Identification of a SialateO-Acetyltransferase fromCampylobacter jejuni
  79. Structural and Functional Characterization of PseC, an Aminotransferase Involved in the Biosynthesis of Pseudaminic Acid, an Essential Flagellar Modification in Helicobacter pylori
  80. Chemical and Chemoenzymatic Synthesis of S‐Linked Ganglioside Analogues and Their Protein Conjugates for Use as Immunogens
  81. Functional Characterization of Dehydratase/Aminotransferase Pairs fromHelicobacterandCampylobacter
  82. Erratum
  83. Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a
  84. Identification and Characterization of NeuB3 fromCampylobacter jejunias a Pseudaminic Acid Synthase
  85. Novel biosynthetic functions of lipopolysaccharide rfaJ homologs from Helicobacter pylori
  86. Structural and Mechanistic Analysis of Sialic Acid Synthase NeuB fromNeisseria meningitidisin Complex with Mn2+, Phosphoenolpyruvate, andN-Acetylmannosaminitol
  87. Analysis of Campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses
  88. A Single Bifunctional UDP-GlcNAc/Glc 4-Epimerase Supports the Synthesis of Three Cell Surface Glycoconjugates inCampylobacter jejuni
  89. Identification and Mechanism of a Bacterial Hydrolyzing UDP-N-Acetylglucosamine 2-Epimerase
  90. Intermediate Trapping on a Mutant Retaining α-Galactosyltransferase Identifies an Unexpected Aspartate Residue
  91. Evidence for Acquisition of the Lipooligosaccharide Biosynthesis Locus in Campylobacter jejuni GB11, a Strain Isolated from a Patient with Guillain-Barré Syndrome, by Horizontal Exchange
  92. Characterization of the  -1,2-N-acetylglucosaminyltransferase of Neisseria gonorrhoeae, a key control point in lipooligosaccharride biosynthesis
  93. Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog
  94. Simplifying Oligosaccharide Synthesis: Efficient Synthesis of Lactosamine and Siaylated Lactosamine Oligosaccharide Donors.
  95. Large‐Scale In Vivo Synthesis of the Carbohydrate Moieties of Gangliosides GM1 and GM2 by Metabolically Engineered Escherichia coli
  96. Simplifying Oligosaccharide Synthesis:  Efficient Synthesis of Lactosamine and Siaylated Lactosamine Oligosaccharide Donors
  97. Preparative-Scale Chemoenzymatic Synthesis of Large Carbohydrate Assemblies Using α(1→4)-Galactosyltransferase⧸UDP-4′-Gal-Epimerase Fusion Protein
  98. One Step Closer to a Sweet Conclusion
  99. Incorporation of N-Acetylneuraminic Acid into Haemophilus somnus Lipooligosaccharide (LOS): Enhancement of Resistance to Serum and Reduction of LOS Antibody Binding
  100. The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168
  101. Incorporation of N-Acetylneuraminic Acid into Haemophilus somnus Lipooligosaccharide (LOS): Enhancement of Resistance to Serum and Reduction of LOS Antibody Binding
  102. High-level expression of recombinant Neisseria CMP-sialic acid synthetase in Escherichia coli
  103. Mechanistic Studies of a Retaining α-Galactosyltransferase from Neisseria meningitidis,
  104. Mechanistic Studies of a Retaining α-Galactosyltransferase from Neisseria meningitidis
  105. The Genetic Bases for the Variation in the Lipo-oligosaccharide of the Mucosal Pathogen,Campylobacter jejuni
  106. Chemoenzymatic Iterative Synthesis of Difficult Linkages of Oligosaccharides on Soluble Polymeric Supports
  107. A Campylobacter jejuni gene associated with immune-mediated neuropathy
  108. Efficient Preparation of Natural and Synthetic Galactosides with a Recombinant β-1,4-Galactosyltransferase-/UDP-4‘-Gal Epimerase Fusion Protein
  109. Dependence of the Bi-functional Nature of a Sialyltransferase fromNeisseria meningitidison a Single Amino Acid Substitution
  110. Identification of a lipopolysaccharide α‐2,3‐sialyltransferase from Haemophilus influenzae
  111. Structure of a Sialic Acid-activating Synthetase, CMP-acylneuraminate Synthetase in the Presence and Absence of CDP
  112. FlaA1, a New Bifunctional UDP-GlcNAc C6Dehydratase/ C4Reductase fromHelicobacter pylori
  113. Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison
  114. Phase variation of a β‐1,3 galactosyltransferase involved in generation of the ganglioside GM1‐like lipo‐oligosaccharide of Campylobacter jejuni
  115. ChemInform Abstract: Enzymatic Synthesis of Oligosaccharide Analogues: Evaluation of UDP‐Gal Analogues as Donors for Three Retaining α‐Galactosyltransferases.
  116. Expression, Purification, and Biochemical Characterization of WbpP, a New UDP-GlcNAc C4 Epimerase fromPseudomonas aeruginosaSerotype O6
  117. Ready Access to Sialylated Oligosaccharide Donors
  118. Functional genomics of Helicobacter pylori: identification of a beta-1,4 galactosyltransferase and generation of mutants with altered lipopolysaccharide
  119. Biosynthesis of Ganglioside Mimics inCampylobacter jejuniOH4384
  120. Enzymatic Synthesis of Oligosaccharide Analogues:  Evaluation of UDP-Gal Analogues as Donors for Three Retaining α-Galactosyltransferases
  121. Glycosyl Fluorides Can Function as Substrates for Nucleotide Phosphosugar-dependent Glycosyltransferases
  122. The synthesis of sialylated oligosaccharides using a CMP-Neu5Ac synthetase/sialyltransferase fusion
  123. Development of an on-line preconcentration method for the analysis of pathogenic lipopolysaccharides using capillary electrophoresis–electrospray mass spectrometry
  124. Scan‐rate dependence in protein calorimetry: The reversible transitions of Bacillus circulans xylanase and a disulfide‐bridge mutant
  125. Structure of an α‐2,6‐sialylated lipooligosaccharide from Neisseria meningitidis immunotype L1
  126. Capsular polysaccharide synthesis in Streptococcus pneumoniae serotype 14: molecular analysis of the complete cps locus and identification of genes encoding glycosyltransferases required for the biosynthesis of the tetrasaccharide subunit
  127. Characterization of a Recombinant Neisseria Meningitidesα‐2,3‐Sialyltransferase and its Acceptor Specificity
  128. Positioning the Acid/Base Catalyst in a Glycosidase:  Studies with Bacillus circulans Xylanase
  129. Cloning of the Lipooligosaccharide α-2,3-Sialyltransferase from the Bacterial PathogensNeisseria meningitidisandNeisseria gonorrhoeae
  130. Characterization of a buried neutral histidine residue inBacillus circulansxylanase: Nmr assignments, pH titration, and hydrogen exchange
  131. Functional Relationships of the Genetic Locus Encoding the Glycosyltransferase Enzymes Involved in Expression of the Lacto-N-neotetraose Terminal Lipopolysaccharide Structure inNeisseria meningitidis
  132. Secondary structure and NMR assignments ofbacillus circulansxylanase
  133. Effects of both Shortening and Lengthening the Active Site Nucleophile of Bacillus circulans Xylanase on Catalytic Activity
  134. The pKaof the General Acid/Base Carboxyl Group of a Glycosidase Cycles during Catalysis:  A13C-NMR Study ofBacillus circulansXylanase
  135. Abnormally High pKa of an Active‐Site Glutamic Acid Residue in Bacillus Circulans Xylanase
  136. Expression of Trichoderma reesei and Trichoderma viride xylanases in Escherichia coli
  137. Mutational and crystallographic analyses of the active site residues of the bacillus circulans xylanase
  138. Thermostabilization of the Bacillus circulansxylanase by the introduction of disulfide bonds
  139. Amino acid sequence and thermostability of xylanase A from schizophyllum commune
  140. Overexpression of the Bacillus subtilis and circulans Xylanases in Escherichia coli
  141. Two GC-rich DNA elements ofChlamydomonas reinhardtii with complex arrangements of directly repeated sequence motifs
  142. Gametic differentiation in Chlamydomonas reinhardtii: light dependence and gene expression patterns
  143. The molecular cloning and expression of a cellobiase gene from anAgrobacterium inEscherichia coli
  144. α-Putrescinylthymine and the sensitivity of bacteriophageφW-14 DNA to restriction endonucleases
  145. The Preliminary Characterization of the  -Glucosidases of Cellulomonas fimi
  146. The Cellulase System of Cellulomonas fimi
  147. Isolation and Characterization of Escherichia coli Clones Expressing Cellulase Genes from Cellulomonas fimi
  148. Capillary Electrophoresis as an Assay Method for Monitoring Glycosyltransferase Activity
  149. Analysis of Bacterial Glycolipids by Capillary Electrophoresis-Electrospray Mass Spectrometry: Haemophilus influenzae and Neisseria meningitidis Lipopolysaccharides