What is it about?

Cancer is currently regarded as one of the primary contributors to mortality and is projected to be the second most common cause of death across the globe, following cardiovascular disease. Natural substances have been administered for hundreds of years, specifically in the field of medicine, and are capable of influencing various cellular signalling pathways, including apoptosis and the cell cycle. For instance, Ganoderma lucidum, commonly referred to as the perennial mushroom, has been utilised in traditional medicine for more than two millennia as an inherent source of active compounds. The objective of this research was to ascertain the fungal extract's impact on colorectal cancer cell cells (HT-29 and HCT-116) regarding in vitro cytotoxicity, genotoxicity, apoptosis, and redox homeostasis outcomes

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Why is it important?

The extract from G. lucidum resulted in an increase in cellular oxidants by decreasing the NRF-2 level. Additionally, a fluctuation in OSI level during oxidative stress analysis indicates a substantial cytotoxic effect. As a result, the findings of the current study support the general suggestion that G. lucidum extract possesses antiproliferative and cytotoxic effects. It is possible that the reduction of NRF-2 by G. lucidum alters redox homeostasis, resulting in heightened cancer cell susceptibility to traditional chemotherapeutics.


The study found that G. lucidum and cisplatin notably reduced the NRF-2 level in HCT-116 and HT-29 CRC cell lines compared to the control group. As a result, these agents could disrupt NRF-2 adaptation in cancer cells, considering the lower NRF-2 level observed in the cisplatin and G. lucidum treated cells, as reported in this study. These findings regarding the decline in the adaptability of NRF-2, the key regulator of redox homeostasis, align with the results of the cytotoxicity test that induced the demise of CRC cells exposed to G. lucidum.

Van Yüzüncü Yıl University

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This page is a summary of: Assessment of redox homeostasis via genotoxicity, cytotoxicity, apoptosis and NRF-2 in colorectal cancer cell lines after treatment with Ganoderma lucidum extract, Drug and Chemical Toxicology, September 2023, Taylor & Francis,
DOI: 10.1080/01480545.2023.2257403.
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