Gamma ray-mediated functionalization of monolithic cryogels for macro-biomolecule purification

Naveenkumar Singh, Roy N. Dsouza, Mariano Grasselli, Marcelo Fernández-Lahore
  • New Biotechnology, July 2014, Elsevier
  • DOI: 10.1016/j.nbt.2014.05.1924

Functionalization of monolithic cryogels for biotherapeutic purification

What is it about?

Recently introduced megaporous cryogels are relatively new and have not been fully exploited for the downstream processing of macro-biomolecules. Weak anion-exchange functionality was incorporated by gamma irradiation-induced grafting. The total ionic capacity obtained for DEAE-functionalized cryogel was 0.59 meq/g. Scanning electron microscopy confirmed that the porous nature of the cryogels remained unaffected by the irradiation-grafting procedure.

Why is it important?

The resulting weak anion-exchange cryogels showed a pore size distribution of up to 100 μm with dynamic binding capacities of ca. 27 ± 3 mg/mL. The dynamic binding capacities of monolithic cryogels were found to be independent of the applied flow rate. Due to the large pores, high permeability, disposability, and cost-efficient nature, megaporous cryogels are an attractive alternative for the purification of macro-biomolecules like mAbs, mRNA, pDNA and viruses.

Perspectives

Dr Naveen Kumar Singh
University of Notre Dame

The adsorbents presented in here showed better column efficiency when compared to packed-bed adsorbents and also had better protein binding capacities.

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http://dx.doi.org/10.1016/j.nbt.2014.05.1924

The following have contributed to this page: Professor Mariano GRASSELLI and Dr Naveen Kumar Singh