Functionalization of monolithic cryogels for biotherapeutic purification
What is it about?
Recently introduced megaporous cryogels are relatively new and have not been fully exploited for the downstream processing of macro-biomolecules. Weak anion-exchange functionality was incorporated by gamma irradiation-induced grafting. The total ionic capacity obtained for DEAE-functionalized cryogel was 0.59 meq/g. Scanning electron microscopy confirmed that the porous nature of the cryogels remained unaffected by the irradiation-grafting procedure.
Why is it important?
The resulting weak anion-exchange cryogels showed a pore size distribution of up to 100 μm with dynamic binding capacities of ca. 27 ± 3 mg/mL. The dynamic binding capacities of monolithic cryogels were found to be independent of the applied flow rate. Due to the large pores, high permeability, disposability, and cost-efficient nature, megaporous cryogels are an attractive alternative for the purification of macro-biomolecules like mAbs, mRNA, pDNA and viruses.
The following have contributed to this page: Professor Mariano GRASSELLI and Dr Naveen Kumar Singh