All Stories

  1. SpyTag-Enabled Assembly of Bacterial Microcompartment Trimers into Macroscopic Layered Protein Materials

    Article • April 2026, openRxiv

    Robert Hausinger

  2. Quantifying selective metabolite transport for the bacterial microcompartment from Haliangium ochraceum with molecular dynamics simulations

    Article • Nanoscale, January 2026, Royal Society of Chemistry

    Robert Hausinger

  3. Quantifying Selective Metabolite Transport for the Bacterial Microcompartment from Haliangium ochraceum with Molecular Dynamics Simulations

    Article • July 2025, American Chemical Society (ACS)

    Robert Hausinger

  4. Ancestral Sequence Reconstruction of the Ethylene-Forming Enzyme

    Article • Biochemistry, July 2025, American Chemical Society (ACS)

    Robert Hausinger

  5. Sumner’s legacy: a century of urease crystals and recent structural advances

    Article • Critical Reviews in Biochemistry and Molecular Biology, May 2025, Taylor & Francis

    Robert Hausinger

  6. Biochemical, Structural, and Conformational Characterization of a Fungal Ethylene-Forming Enzyme

    Article • Biochemistry, March 2025, American Chemical Society (ACS)

    Robert Hausinger

  7. Overcoming barriers for investigating nickel-pincer nucleotide cofactor-related enzymes

    Article • mBio, February 2025, ASM Journals

    Robert Hausinger

  8. Structural Basis for the Catalysis and Substrate Specificity of a LarA Racemase with a Broad Substrate Spectrum

    Article • ACS Catalysis, February 2025, American Chemical Society (ACS)

    Robert Hausinger

  9. A structural view of nickel-pincer nucleotide cofactor-related biochemistry

    Article • Critical Reviews in Biochemistry and Molecular Biology, November 2024, Taylor & Francis

    Robert Hausinger

  10. EvoSeq-ML: Advancing Data-Centric Machine Learning with Evolutionary-Informed Protein Sequence Representation and Generation

    Article • October 2024, openRxiv

    Robert Hausinger

  11. Structural, Spectroscopic, and Computational Insights from Canavanine-Bound and Two Catalytically Compromised Variants of the Ethylene-Forming Enzyme

    Article • Biochemistry, April 2024, American Chemical Society (ACS)

    Robert Hausinger

  12. Bile salt hydrolase acyltransferase activity expands bile acid diversity

    Article • Nature, February 2024, Springer Science + Business Media

    Robert Hausinger

  13. Biosynthesis and Functions of the Nickel-Pincer Nucleotide (NPN) Cofactor

    Article • January 2024, Cassyni

    Robert Hausinger

  14. Structure of the LarB–Substrate Complex and Identification of a Reaction Intermediate during Nickel-Pincer Nucleotide Cofactor Biosynthesis

    Article • Biochemistry, October 2023, American Chemical Society (ACS)

    Robert Hausinger

  15. Dioxygen Binding Is Controlled by the Protein Environment in Non‐heme FeII and 2‐Oxoglutarate Oxygenases: A Study on Histone Demethylase PHF8 and an Ethylene‐Forming Enzyme

    Article • Chemistry - A European Journal, February 2023, Wiley

    Robert Hausinger

  16. Irreversible Inactivation of Lactate Racemase by Sodium Borohydride Reveals Reactivity of the Nickel–Pincer Nucleotide Cofactor

    Article • ACS Catalysis, January 2023, American Chemical Society (ACS)

    Robert Hausinger

  17. Biological formation of ethylene

    Article • RSC Chemical Biology, January 2023, Royal Society of Chemistry

    Robert Hausinger

  18. Can an external electric field switch between ethylene formation and l-arginine hydroxylation in the ethylene forming enzyme?

    Article • Physical Chemistry Chemical Physics, January 2023, Royal Society of Chemistry

    Robert Hausinger

  19. The nickel-pincer coenzyme of lactate racemase: A case study of uncovering cofactor structure and biosynthesis

    Article • January 2023, Elsevier

    Robert Hausinger

  20. Five decades of metalloenzymology

    Article • January 2023, Elsevier

    Robert Hausinger

  21. Sulfur incorporation into biomolecules: recent advances

    Article • Critical Reviews in Biochemistry and Molecular Biology, November 2022, Taylor & Francis

    Robert Hausinger

  22. Bile salt hydrolase/aminoacyltransferase shapes the microbiome

    Article • October 2022, Research Square

    Robert Hausinger

  23. Unveiling the mechanisms and biosynthesis of a novel nickel-pincer enzyme

    Article • Biochemical Society Transactions, August 2022, Portland Press Ltd.

    Robert Hausinger

  24. Characterization of a [4Fe-4S]-dependent LarE sulfur insertase that facilitates nickel-pincer nucleotide cofactor biosynthesis in Thermotoga maritima

    Article • Journal of Biological Chemistry, July 2022, Elsevier

    Robert Hausinger

  25. Characterization of the nickel-inserting cyclometallase LarC from Moorella thermoacetica and identification of a cytidinylylated reaction intermediate

    Article • Metallomics, February 2022, Oxford University Press (OUP)

    Robert Hausinger

  26. Structural and mutational characterization of a malate racemase from the LarA superfamily

    Article • BioMetals, February 2022, Springer Science + Business Media

    Robert Hausinger

  27. Iron-containing ureases

    Article • Coordination Chemistry Reviews, December 2021, Elsevier

    Robert Hausinger

  28. The LarB carboxylase/hydrolase forms a transient cysteinyl-pyridine intermediate during nickel-pincer nucleotide cofactor biosynthesis

    Article • Proceedings of the National Academy of Sciences, September 2021, Proceedings of the National Academy of Sciences

    Robert Hausinger

  29. Atomic and Electronic Structure Determinants Distinguish between Ethylene Formation and l-Arginine Hydroxylation Reaction Mechanisms in the Ethylene-Forming Enzyme

    Article • ACS Catalysis, January 2021, American Chemical Society (ACS)

    Robert Hausinger

  30. 1H-HYSCORE Reveals Structural Details at the Fe(II) Active Site of Taurine:2-Oxoglutarate Dioxygenase

    Article • Applied Magnetic Resonance, October 2020, Springer Science + Business Media

    Robert Hausinger

  31. Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases

    Article • Scientific Reports, October 2020, Springer Science + Business Media

    Robert Hausinger

  32. Biological Pincer Complexes

    Article • ChemCatChem, June 2020, Wiley

    Robert Hausinger

  33. Lanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function

    Article • Journal of Biological Chemistry, June 2020, Elsevier

    Robert Hausinger

  34. Crystallographic characterization of a tri-Asp metal-binding site at the three-fold symmetry axis of LarE

    Article • Scientific Reports, April 2020, Springer Science + Business Media

    Robert Hausinger

  35. Nickel-Pincer Nucleotide Cofactor-Containing Enzymes

    Article • January 2020, Elsevier

    Robert Hausinger

  36. New metal cofactors and recent metallocofactor insights

    Article • Current Opinion in Structural Biology, December 2019, Elsevier

    Robert Hausinger

  37. Structural Origin of the Large Redox-Linked Reorganization in the 2-Oxoglutarate Dependent Oxygenase, TauD

    Article • Journal of the American Chemical Society, September 2019, American Chemical Society (ACS)

    Robert Hausinger

  38. Strongly Coupled Redox-Linked Conformational Switching at the Active Site of the Non-Heme Iron-Dependent Dioxygenase, TauD

    Article • The Journal of Physical Chemistry B, August 2019, American Chemical Society (ACS)

    Robert Hausinger

  39. A structural perspective on the PP-loop ATP pyrophosphatase family

    Article • Critical Reviews in Biochemistry and Molecular Biology, October 2018, Taylor & Francis

    Jian Hu, Robert Hausinger

  40. Thermodynamics of Iron(II) and Substrate Binding to the Ethylene-Forming Enzyme

    Article • Biochemistry, September 2018, American Chemical Society (ACS)

    Robert Hausinger

  41. Analysis of the Active Site Cysteine Residue of the Sacrificial Sulfur Insertase LarE from Lactobacillus plantarum

    Article • Biochemistry, August 2018, American Chemical Society (ACS)

    Robert Hausinger

  42. Biosynthesis of the nickel-pincer nucleotide cofactor of lactate racemase requires a CTP-dependent cyclometallase

    Article • Journal of Biological Chemistry, August 2018, Elsevier

    Robert Hausinger

  43. Lactate Racemase Nickel-Pincer Cofactor Operates by a Proton-Coupled Hydride Transfer Mechanism

    Article • Biochemistry, February 2018, American Chemical Society (ACS)

    Robert Hausinger

  44. Characterization of human AlkB homolog 1 produced in mammalian cells and demonstration of mitochondrial dysfunction in ALKBH1-deficient cells

    Article • Biochemical and Biophysical Research Communications, January 2018, Elsevier

    Robert Hausinger

  45. Structures and Mechanisms of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme: Substrate Binding Creates a Twist

    Article • Journal of the American Chemical Society, August 2017, American Chemical Society (ACS)

    Robert Hausinger

  46. Structural insights into the catalytic mechanism of a sacrificial sulfur insertase of the N-type ATP pyrophosphatase family, LarE

    Article • Proceedings of the National Academy of Sciences, August 2017, Proceedings of the National Academy of Sciences

    Robert Hausinger

  47. Correction to Biochemical and Spectroscopic Characterization of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme from Pseudomonas syringae pv. phaseolicola PK2

    Article • Biochemistry, June 2017, American Chemical Society (ACS)

    Robert Hausinger

  48. Biochemical Characterization of AP Lyase and m6A Demethylase Activities of Human AlkB Homologue 1 (ALKBH1)

    Article • Biochemistry, March 2017, American Chemical Society (ACS)

    Robert Hausinger

  49. ALKBH7 Variant Related to Prostate Cancer Exhibits Altered Substrate Binding

    Article • PLoS Computational Biology, February 2017, PLOS

    Robert Hausinger

  50. Nickel-pincer cofactor biosynthesis involves LarB-catalyzed pyridinium carboxylation and LarE-dependent sacrificial sulfur insertion

    Article • Proceedings of the National Academy of Sciences, April 2016, Proceedings of the National Academy of Sciences

    Robert Hausinger

  51. Mutational and Computational Evidence That a Nickel-Transfer Tunnel in UreD Is Used for Activation of Klebsiella aerogenes Urease

    Article • Biochemistry, October 2015, American Chemical Society (ACS)

    Robert Hausinger

  52. Catalytic Mechanisms of Fe(II)- and 2-Oxoglutarate-dependent Oxygenases

    Article • Journal of Biological Chemistry, August 2015, Elsevier

    Robert Hausinger

  53. A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase

    Article • Science, July 2015, American Association for the Advancement of Science

    Robert Hausinger

  54. Calorimetric Assessment of Fe2+ Binding to α-Ketoglutarate/Taurine Dioxygenase: Ironing Out the Energetics of Metal Coordination by the 2-His-1-Carboxylate Facial Triad

    Article • Inorganic Chemistry, February 2015, American Chemical Society (ACS)

    Robert Hausinger

  55. Reduction of Urease Activity by Interaction with the Flap Covering the Active Site

    Article • Journal of Chemical Information and Modeling, January 2015, American Chemical Society (ACS)

    Robert Hausinger

  56. Homology modeling, molecular dynamics, and site-directed mutagenesis study of AlkB human homolog 1 (ALKBH1)

    Article • Journal of Molecular Graphics and Modelling, November 2014, Elsevier

    Robert Hausinger

  57. Sustained photobiological hydrogen production in the presence of N2 by nitrogenase mutants of the heterocyst-forming cyanobacterium Anabaena

    Article • International Journal of Hydrogen Energy, November 2014, Elsevier

    Robert Hausinger

  58. Mechanism of the 6-Hydroxy-3-succinoyl-pyridine 3-Monooxygenase Flavoprotein from Pseudomonas putida S16

    Article • Journal of Biological Chemistry, October 2014, Elsevier

    Robert Hausinger

  59. Nickel-dependent metalloenzymes

    Article • Archives of Biochemistry and Biophysics, February 2014, Elsevier

    Robert Hausinger

  60. Measuring the Orientation of Taurine in the Active Site of the Non-Heme Fe(II)/α-Ketoglutarate-Dependent Taurine Hydroxylase (TauD) Using Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy

    Article • The Journal of Physical Chemistry B, August 2013, American Chemical Society (ACS)

    Robert Hausinger

  61. Analysis of a Soluble (UreD:UreF:UreG)2 Accessory Protein Complex and Its Interactions with Klebsiella aerogenes Urease by Mass Spectrometry

    Article • Journal of the American Society for Mass Spectrometry, June 2013, American Chemical Society (ACS)

    Robert Hausinger

  62. ALKBH1 Is Dispensable for Abasic Site Cleavage during Base Excision Repair and Class Switch Recombination

    Article • PLOS One, June 2013, PLOS

    Robert Hausinger

  63. A covalent protein–DNA 5′-product adduct is generated following AP lyase activity of human ALKBH1 (AlkB homologue 1)

    Article • Biochemical Journal, May 2013, Portland Press Ltd.

    Robert Hausinger

  64. Biosynthesis of the Urease Metallocenter

    Article • Journal of Biological Chemistry, May 2013, Elsevier

    Robert Hausinger

  65. Nickel-Binding Sites in Proteins

    Article • January 2013, Springer Science + Business Media

    Robert Hausinger

  66. Apoprotein isolation and activation, and vibrational structure of the Helicobacter mustelae iron urease

    Article • Journal of Inorganic Biochemistry, June 2012, Elsevier

    Robert Hausinger

  67. Characterization of a Trypanosoma brucei Alkb homolog capable of repairing alkylated DNA

    Article • Experimental Parasitology, May 2012, Elsevier

    Robert Hausinger

  68. Klebsiella aerogenes UreF: Identification of the UreG Binding Site and Role in Enhancing the Fidelity of Urease Activation

    Article • Biochemistry, March 2012, American Chemical Society (ACS)

    Robert Hausinger

  69. Genetic Engineering of Cyanobacteria to Enhance Biohydrogen Production from Sunlight and Water

    Article • AMBIO, March 2012, Springer Science + Business Media

    Robert Hausinger

  70. Fructose-1,6-bisphosphate aldolase (class II) is the primary site of nickel toxicity in Escherichia coli

    Article • Molecular Microbiology, November 2011, Wiley

    Robert Hausinger

  71. Function of UreB in Klebsiella aerogenes Urease

    Article • Biochemistry, October 2011, American Chemical Society (ACS)

    Robert Hausinger

  72. The Escherichia coli alkylation response protein AidB is a redox partner of flavodoxin and binds RNA and acyl carrier protein

    Article • Archives of Biochemistry and Biophysics, September 2011, Elsevier

    Robert Hausinger

  73. Iron-containing urease in a pathogenic bacterium

    Article • Proceedings of the National Academy of Sciences, July 2011, Proceedings of the National Academy of Sciences

    Robert Hausinger

  74. Mechanisms of nickel toxicity in microorganisms

    Article • Metallomics, January 2011, Oxford University Press (OUP)

    Robert Hausinger

  75. Crystal structure of a truncated urease accessory protein UreF from Helicobacter pylori

    Article • Proteins Structure Function and Bioinformatics, August 2010, Wiley

    Jennifer Guthrie, Robert Hausinger

  76. Site-Directed Mutagenesis of the Anabaena sp. Strain PCC 7120 Nitrogenase Active Site To Increase Photobiological Hydrogen Production

    Article • Applied and Environmental Microbiology, August 2010, ASM Journals

    Robert Hausinger

  77. Mutagenesis of Klebsiella aerogenes UreG To Probe Nickel Binding and Interactions with Other Urease-Related Proteins

    Article • Biochemistry, June 2010, American Chemical Society (ACS)

    Robert Hausinger

  78. Metal and substrate binding to an Fe(II) dioxygenase resolved by UV spectroscopy with global regression analysis

    Article • Analytical Biochemistry, April 2010, Elsevier

    Robert Hausinger

  79. Trypanosoma brucei brucei: Thymine 7-hydroxylase-like proteins

    Article • Experimental Parasitology, April 2010, Elsevier

    Robert Hausinger

  80. Characterization of the Klebsiella aerogenes Urease Accessory Protein UreD in Fusion with the Maltose Binding Protein

    Article • Journal of Bacteriology, March 2010, ASM Journals

    Robert Hausinger

  81. Insight into the mechanism of an iron dioxygenase by resolution of steps following the Fe IV ═O species

    Article • Proceedings of the National Academy of Sciences, February 2010, Proceedings of the National Academy of Sciences

    Robert Hausinger

  82. Human AlkB homologue 1 (ABH1) exhibits DNA lyase activity at abasic sites

    Article • DNA Repair, January 2010, Elsevier

    Robert Hausinger

  83. Interplay of metal ions and urease

    Article • Metallomics, January 2009, Oxford University Press (OUP)

    Robert Hausinger

  84. The structure of urease activation complexes examined by flexibility analysis, mutagenesis, and small-angle X-ray scattering

    Article • Archives of Biochemistry and Biophysics, December 2008, Elsevier

    Robert Hausinger

  85. Identification of Escherichia coli YgaF as an l-2-Hydroxyglutarate Oxidase

    Article • Journal of Bacteriology, April 2008, ASM Journals

    Robert Hausinger

  86. Characterization of active site variants of xanthine hydroxylase from Aspergillus nidulans

    Article • Archives of Biochemistry and Biophysics, February 2008, Elsevier

    Robert Hausinger

  87. FeII/α-ketoglutarate hydroxylases involved in nucleobase, nucleoside, nucleotide, and chromatin metabolism

    Article • Dalton Transactions, January 2008, Royal Society of Chemistry

    Robert Hausinger

  88. CrII Reactivity of Taurine/α-Ketoglutarate Dioxygenase

    Article • Inorganic Chemistry, November 2007, American Chemical Society (ACS)

    Robert Hausinger

  89. Thermodynamics of Ni2+, Cu2+, and Zn2+ Binding to the Urease Metallochaperone UreE

    Article • Biochemistry, August 2007, American Chemical Society (ACS)

    Robert Hausinger

  90. Metal ligand substitution and evidence for quinone formation in taurine/α-ketoglutarate dioxygenase

    Article • Journal of Inorganic Biochemistry, May 2007, Elsevier

    Robert Hausinger

  91. Probing the Iron−Substrate Orientation for Taurine/α-Ketoglutarate Dioxygenase Using Deuterium Electron Spin Echo Envelope Modulation Spectroscopy

    Article • Biochemistry, May 2007, American Chemical Society (ACS)

    Robert Hausinger

  92. Purification and Characterization of the FeII- and α-Ketoglutarate-Dependent Xanthine Hydroxylase from Aspergillus nidulans

    Article • Biochemistry, April 2007, American Chemical Society (ACS)

    Robert Hausinger

  93. The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase

    Article • Nucleic Acids Research, March 2007, Oxford University Press (OUP)

    Dr Anastassis Perrakis, Robert Hausinger

  94. New Insights into Acetone Metabolism▿

    Article • Journal of Bacteriology, December 2006, ASM Journals

    Robert Hausinger

  95. The UreEF Fusion Protein Provides a Soluble and Functional Form of the UreF Urease Accessory Protein

    Article • Journal of Bacteriology, October 2006, ASM Journals

    Robert Hausinger

  96. Inhibition of urease by bismuth(III): Implications for the mechanism of action of bismuth drugs

    Article • BioMetals, October 2006, Springer Science + Business Media

    Chair Professor of Chemistry Hongzhe Sun, Robert Hausinger

  97. An assay for Fe(II)/2-oxoglutarate-dependent dioxygenases by enzyme-coupled detection of succinate formation

    Article • Analytical Biochemistry, June 2006, Elsevier

    Robert Hausinger

  98. Structural basis for the enantiospecificities ofR- andS-specific phenoxypropionate/α-ketoglutarate dioxygenases

    Article • Protein Science, June 2006, Wiley

    Robert Hausinger

  99. The AidB Component of the Escherichia coli Adaptive Response to Alkylating Agents Is a Flavin-Containing, DNA-Binding Protein

    Article • Journal of Bacteriology, January 2006, ASM Journals

    Robert Hausinger

  100. Self-hydroxylation of taurine/α-ketoglutarate dioxygenase: evidence for more than one oxygen activation mechanism

    Article • JBIC Journal of Biological Inorganic Chemistry, December 2005, Springer Science + Business Media

    Robert Hausinger

  101. Kinetic and spectroscopic investigation of CoII, NiII, and N-oxalylglycine inhibition of the FeII/α-ketoglutarate dioxygenase, TauD

    Article • Biochemical and Biophysical Research Communications, December 2005, Elsevier

    Robert Hausinger

  102. Biosynthesis of Active Bacillus subtilis Urease in the Absence of Known Urease Accessory Proteins

    Article • Journal of Bacteriology, October 2005, ASM Journals

    Robert Hausinger

  103. Purification and Properties of the Klebsiella aerogenes UreE Metal-Binding Domain, a Functional Metallochaperone of Urease

    Article • Journal of Bacteriology, May 2005, ASM Journals

    Robert Hausinger

  104. Steady-State and Transient Kinetic Analyses of Taurine/α-Ketoglutarate Dioxygenase:  Effects of Oxygen Concentration, Alternative Sulfonates, and Active-Site Variants on the FeIV-oxo Intermediate

    Article • Biochemistry, February 2005, American Chemical Society (ACS)

    Robert Hausinger

  105. Biosynthesis of Metal Sites

    Article • ChemInform, May 2004, Wiley

    Robert Hausinger

  106. Metabolic Versatility of Prokaryotes for Urea Decomposition

    Article • Journal of Bacteriology, May 2004, ASM Journals

    Robert Hausinger

  107. Aberrant activity of the DNA repair enzyme AlkB

    Article • Journal of Inorganic Biochemistry, May 2004, Elsevier

    Robert Hausinger

  108. Chemical Cross-linking and Mass Spectrometric Identification of Sites of Interaction for UreD, UreF, and Urease

    Article • Journal of Biological Chemistry, April 2004, Elsevier

    Robert Hausinger

  109. Direct Detection of Oxygen Intermediates in the Non-Heme Fe Enzyme Taurine/α-Ketoglutarate Dioxygenase

    Article • Journal of the American Chemical Society, January 2004, American Chemical Society (ACS)

    Robert Hausinger

  110. Fe(II)/α-Ketoglutarate-Dependent Hydroxylases and Related Enzymes

    Article • Critical Reviews in Biochemistry and Molecular Biology, January 2004, Taylor & Francis

    Robert Hausinger

  111. Biosynthesis of Metal Sites

    Article • Chemical Reviews, December 2003, American Chemical Society (ACS)

    Robert Hausinger

  112. Nickel uptake and utilization by microorganisms

    Article • FEMS Microbiology Reviews, June 2003, Oxford University Press (OUP)

    Robert Hausinger

  113. Ni and CO: more surprises

    Article • Nature Structural & Molecular Biology, April 2003, Springer Science + Business Media

    Robert Hausinger

  114. Interconversion of two oxidized forms of taurine/α-ketoglutarate dioxygenase, a non-heme iron hydroxylase: Evidence for bicarbonate binding

    Article • Proceedings of the National Academy of Sciences, March 2003, Proceedings of the National Academy of Sciences

    Robert Hausinger

  115. O2- and α-Ketoglutarate-Dependent Tyrosyl Radical Formation in TauD, an α-Keto Acid-Dependent Non-Heme Iron Dioxygenase

    Article • Biochemistry, January 2003, American Chemical Society (ACS)

    Robert Hausinger

  116. Metal Ion Dependence of Recombinant Escherichia coli Allantoinase

    Article • Journal of Bacteriology, January 2003, ASM Journals

    Robert Hausinger

  117. Intrinsic tryptophan fluorescence as a probe of metal and α-ketoglutarate binding to TfdA, a mononuclear non-heme iron dioxygenase

    Article • Journal of Inorganic Biochemistry, January 2003, Elsevier

    Deborah A Hogan, Robert Hausinger

  118. Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage

    Article • Nature, September 2002, Springer Science + Business Media

    Robert Hausinger

  119. Probing the 2,4-Dichlorophenoxyacetate/α-Ketoglutarate Dioxygenase Substrate-Binding Site by Site-Directed Mutagenesis and Mechanism-Based Inactivation

    Article • Biochemistry, July 2002, American Chemical Society (ACS)

    Robert Hausinger

  120. tfdA-Like Genes in 2,4-Dichlorophenoxyacetic Acid-Degrading Bacteria Belonging to the Bradyrhizobium-Agromonas-Nitrobacter-Afipia Cluster in α-Proteobacteria

    Article • Applied and Environmental Microbiology, July 2002, ASM Journals

    Robert Hausinger

  121. Non-heme iron oxygenases

    Article • Current Opinion in Chemical Biology, April 2002, Elsevier

    Robert Hausinger

  122. X-ray Crystal Structure of Escherichia coli Taurine/α-Ketoglutarate Dioxygenase Complexed to Ferrous Iron and Substrates,

    Article • Biochemistry, March 2002, American Chemical Society (ACS)

    Robert Hausinger

  123. Crystal Structure of Klebsiella aerogenesUreE, a Nickel-binding Metallochaperone for Urease Activation

    Article • Journal of Biological Chemistry, October 2001, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor Hyun Kyu Song, Robert Hausinger

  124. Dual Effects of Ionic Strength on Klebsiella aerogenes Urease: pH-Dependent Activation and Inhibition

    Article • Archives of Biochemistry and Biophysics, October 2001, Elsevier

    Robert Hausinger

  125. Alternative Reactivity of an α-Ketoglutarate-Dependent Iron(II) Oxygenase:  Enzyme Self-Hydroxylation

    Article • Journal of the American Chemical Society, May 2001, American Chemical Society (ACS)

    Robert Hausinger

  126. Resonance Raman Studies of the Iron(II)−α-Keto Acid Chromophore in Model and Enzyme Complexes

    Article • Journal of the American Chemical Society, May 2001, American Chemical Society (ACS)

    Robert Hausinger

  127. UreE Stimulation of GTP-Dependent Urease Activation in the UreD-UreF-UreG-urease Apoprotein Complex

    Article • Biochemistry, September 2000, American Chemical Society (ACS)

    Robert Hausinger

  128. Kinetic and Structural Characterization of Urease Active Site Variants,

    Article • Biochemistry, June 2000, American Chemical Society (ACS)

    Robert Hausinger

  129. Fluoride Inhibition of Klebsiella aerogenes Urease:  Mechanistic Implications of a Pseudo-uncompetitive, Slow-Binding Inhibitor

    Article • Biochemistry, April 2000, American Chemical Society (ACS)

    Robert Hausinger

  130. In Vivo and in Vitro Kinetics of Metal Transfer by the Klebsiella aerogenes Urease Nickel Metallochaperone, UreE

    Article • Journal of Biological Chemistry, April 2000, Elsevier

    Robert Hausinger

  131. Site-directed Mutagenesis of 2,4-Dichlorophenoxyacetic Acid/α-Ketoglutarate Dioxygenase

    Article • Journal of Biological Chemistry, April 2000, Elsevier

    Robert Hausinger

  132. Herbicide-Degrading α-Keto Acid-Dependent Enzyme TfdA:  Metal Coordination Environment and Mechanistic Insights

    Article • Biochemistry, November 1999, American Chemical Society (ACS)

    Robert Hausinger

  133. Stopped-Flow Kinetic Analysis ofEscherichia coliTaurine/α-Ketoglutarate Dioxygenase:  Interactions with α-Ketoglutarate, Taurine, and Oxygen

    Article • Biochemistry, October 1999, American Chemical Society (ACS)

    Robert Hausinger

  134. GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins

    Article • Proceedings of the National Academy of Sciences, September 1999, Proceedings of the National Academy of Sciences

    Robert Hausinger

  135. Characterization of metal-substituted Klebsiella aerogenes urease

    Article • JBIC Journal of Biological Inorganic Chemistry, August 1999, Springer Science + Business Media

    Robert Hausinger

  136. Assays for Allantoinase

    Article • Analytical Biochemistry, March 1999, Elsevier

    Robert Hausinger

  137. Identification of Metal-Binding Residues in the Klebsiella aerogenes Urease Nickel Metallochaperone, UreE

    Article • Biochemistry, March 1999, American Chemical Society (ACS)

    Robert Hausinger

  138. X-ray absorption spectroscopic analysis of Fe(II) and Cu(II) forms of a herbicide-degrading α-ketoglutarate dioxygenase

    Article • JBIC Journal of Biological Inorganic Chemistry, February 1999, Springer Science + Business Media

    Robert Hausinger

  139. Chemical Rescue of Klebsiella aerogenes Urease Variants Lacking the Carbamylated-Lysine Nickel Ligand,

    Article • Biochemistry, April 1998, American Chemical Society (ACS)

    Robert Hausinger

  140. Ascorbic Acid-Dependent Turnover and Reactivation of 2,4-Dichlorophenoxyacetic Acid/α-Ketoglutarate Dioxygenase Using Thiophenoxyacetic Acid

    Article • Biochemistry, February 1998, American Chemical Society (ACS)

    Robert Hausinger

  141. Substitution of the Urease Active Site Carbamate by Dithiocarbamate and Vanadate

    Article • Biochemistry, December 1997, American Chemical Society (ACS)

    Robert Hausinger

  142. Distribution of the tfdA Gene in Soil Bacteria That Do Not Degrade 2,4-Dichlorophenoxyacetic Acid (2,4-D)

    Article • Microbial Ecology, September 1997, Springer Science + Business Media

    Robert Hausinger, Professor Thomas M Schmidt

  143. Structures of Cys319 Variants and Acetohydroxamate-Inhibited Klebsiella aerogenes Urease,

    Article • Biochemistry, July 1997, American Chemical Society (ACS)

    Robert Hausinger

  144. Characterization of the Mononickel Metallocenter in H134A Mutant Urease

    Article • Journal of Biological Chemistry, August 1996, Elsevier

    Robert Hausinger

  145. Metal Ion Interactions with Urease and UreD-Urease Apoproteins

    Article • Biochemistry, January 1996, American Chemical Society (ACS)

    Robert Hausinger

  146. Urease activity in the crystalline state

    Article • Protein Science, October 1995, Wiley

    Robert Hausinger

  147. Characterization of the first enzyme in 2,4-dichlorophenoxyacetic acid metabolism.

    Article • Environmental Health Perspectives, June 1995, Environmental Health Perspectives

    Robert Hausinger

  148. The crystal structure of urease from Klebsiella aerogenes

    Article • Science, May 1995, American Association for the Advancement of Science

    Robert Hausinger

  149. Requirement of Carbon Dioxide for in Vitro Assembly of the Urease Nickel Metallocenter

    Article • Science, February 1995, American Association for the Advancement of Science

    Robert Hausinger

  150. Nickel enzymes in microbes

    Article • The Science of The Total Environment, June 1994, Elsevier

    Robert Hausinger

  151. In vitro activation of urease apoprotein and role of UreD as a chaperone required for nickel metallocenter assembly.

    Article • Proceedings of the National Academy of Sciences, April 1994, Proceedings of the National Academy of Sciences

    Robert Hausinger

  152. Purification and characterization ofKlebsiella aerogenesUreE protein: A nickel-binding protein that functions in urease metallocenter assembly

    Article • Protein Science, June 1993, Wiley

    Robert Hausinger

  153. Site-directed mutagenesis ofKlebsiella aerogenesurease: Identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis

    Article • Protein Science, June 1993, Wiley

    Robert Hausinger

  154. Diethylpyrocarbonate reactivity ofKlebsiella aerogenes urease: Effect ofpH and active site ligands on the rate of inactivation

    Article • The Protein Journal, February 1993, Springer Science + Business Media

    Robert Hausinger

  155. Biochemistry of Nickel

    Article • January 1993, Springer Science + Business Media

    Robert Hausinger

  156. Hydrogenase

    Article • January 1993, Springer Science + Business Media

    Robert Hausinger

  157. Methyl Coenzyme M Reductase

    Article • January 1993, Springer Science + Business Media

    Robert Hausinger

  158. Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes

    Article • Journal of Molecular Biology, October 1992, Elsevier

    Robert Hausinger

  159. Characterization of urease from Sporosarcina ureae

    Article • Archives of Microbiology, May 1992, Springer Science + Business Media

    Robert Hausinger

  160. [33] Separation of flavins and flavin analogs by high-performance liquid chromatography

    Article • January 1986, Elsevier

    Robert Hausinger

  161. Factor 390 chromophores: phosphodiester between AMP or GMP and methanogenic factor 420

    Article • Biochemistry, March 1985, American Chemical Society (ACS)

    Robert Hausinger

  162. Paramagnetic centers in the nickel-containing, deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum

    Article • Proceedings of the National Academy of Sciences, January 1983, Proceedings of the National Academy of Sciences

    Robert Hausinger

  163. Comparison of the iron proteins from the nitrogen fixation complexes of Azotobacter vinelandii, Clostridium pasteurianum , and Klebsiella pneumoniae

    Article • Proceedings of the National Academy of Sciences, July 1980, Proceedings of the National Academy of Sciences

    Robert Hausinger