All Stories

  1. Quantifying Selective Metabolite Transport for the Bacterial Microcompartment from Haliangium ochraceum with Molecular Dynamics Simulations
  2. Ancestral Sequence Reconstruction of the Ethylene-Forming Enzyme
  3. Sumner’s legacy: a century of urease crystals and recent structural advances
  4. Biochemical, Structural, and Conformational Characterization of a Fungal Ethylene-Forming Enzyme
  5. Overcoming barriers for investigating nickel-pincer nucleotide cofactor-related enzymes
  6. Structural Basis for the Catalysis and Substrate Specificity of a LarA Racemase with a Broad Substrate Spectrum
  7. A structural view of nickel-pincer nucleotide cofactor-related biochemistry
  8. Structural, Spectroscopic, and Computational Insights from Canavanine-Bound and Two Catalytically Compromised Variants of the Ethylene-Forming Enzyme
  9. Bile salt hydrolase acyltransferase activity expands bile acid diversity
  10. Biosynthesis and Functions of the Nickel-Pincer Nucleotide (NPN) Cofactor
  11. Structure of the LarB–Substrate Complex and Identification of a Reaction Intermediate during Nickel-Pincer Nucleotide Cofactor Biosynthesis
  12. Dioxygen Binding Is Controlled by the Protein Environment in Non‐heme FeII and 2‐Oxoglutarate Oxygenases: A Study on Histone Demethylase PHF8 and an Ethylene‐Forming Enzyme
  13. Irreversible Inactivation of Lactate Racemase by Sodium Borohydride Reveals Reactivity of the Nickel–Pincer Nucleotide Cofactor
  14. Biological formation of ethylene
  15. Can an external electric field switch between ethylene formation and l-arginine hydroxylation in the ethylene forming enzyme?
  16. The nickel-pincer coenzyme of lactate racemase: A case study of uncovering cofactor structure and biosynthesis
  17. Five decades of metalloenzymology
  18. Sulfur incorporation into biomolecules: recent advances
  19. Bile salt hydrolase/aminoacyltransferase shapes the microbiome
  20. Unveiling the mechanisms and biosynthesis of a novel nickel-pincer enzyme
  21. Characterization of a [4Fe-4S]-dependent LarE sulfur insertase that facilitates nickel-pincer nucleotide cofactor biosynthesis in Thermotoga maritima
  22. Characterization of the nickel-inserting cyclometallase LarC from Moorella thermoacetica and identification of a cytidinylylated reaction intermediate
  23. Structural and mutational characterization of a malate racemase from the LarA superfamily
  24. Iron-containing ureases
  25. The LarB carboxylase/hydrolase forms a transient cysteinyl-pyridine intermediate during nickel-pincer nucleotide cofactor biosynthesis
  26. Atomic and Electronic Structure Determinants Distinguish between Ethylene Formation and l-Arginine Hydroxylation Reaction Mechanisms in the Ethylene-Forming Enzyme
  27. 1H-HYSCORE Reveals Structural Details at the Fe(II) Active Site of Taurine:2-Oxoglutarate Dioxygenase
  28. Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases
  29. Biological Pincer Complexes
  30. Lanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function
  31. Crystallographic characterization of a tri-Asp metal-binding site at the three-fold symmetry axis of LarE
  32. Nickel-Pincer Nucleotide Cofactor-Containing Enzymes
  33. New metal cofactors and recent metallocofactor insights
  34. Structural Origin of the Large Redox-Linked Reorganization in the 2-Oxoglutarate Dependent Oxygenase, TauD
  35. Strongly Coupled Redox-Linked Conformational Switching at the Active Site of the Non-Heme Iron-Dependent Dioxygenase, TauD
  36. A structural perspective on the PP-loop ATP pyrophosphatase family
  37. Thermodynamics of Iron(II) and Substrate Binding to the Ethylene-Forming Enzyme
  38. Analysis of the Active Site Cysteine Residue of the Sacrificial Sulfur Insertase LarE from Lactobacillus plantarum
  39. Biosynthesis of the nickel-pincer nucleotide cofactor of lactate racemase requires a CTP-dependent cyclometallase
  40. Lactate Racemase Nickel-Pincer Cofactor Operates by a Proton-Coupled Hydride Transfer Mechanism
  41. Characterization of human AlkB homolog 1 produced in mammalian cells and demonstration of mitochondrial dysfunction in ALKBH1-deficient cells
  42. Structures and Mechanisms of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme: Substrate Binding Creates a Twist
  43. Structural insights into the catalytic mechanism of a sacrificial sulfur insertase of the N-type ATP pyrophosphatase family, LarE
  44. Correction to Biochemical and Spectroscopic Characterization of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme from Pseudomonas syringae pv. phaseolicola PK2
  45. Biochemical Characterization of AP Lyase and m6A Demethylase Activities of Human AlkB Homologue 1 (ALKBH1)
  46. ALKBH7 Variant Related to Prostate Cancer Exhibits Altered Substrate Binding
  47. Nickel-pincer cofactor biosynthesis involves LarB-catalyzed pyridinium carboxylation and LarE-dependent sacrificial sulfur insertion
  48. Mutational and Computational Evidence That a Nickel-Transfer Tunnel in UreD Is Used for Activation of Klebsiella aerogenes Urease
  49. Catalytic Mechanisms of Fe(II)- and 2-Oxoglutarate-dependent Oxygenases
  50. A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase
  51. Calorimetric Assessment of Fe2+ Binding to α-Ketoglutarate/Taurine Dioxygenase: Ironing Out the Energetics of Metal Coordination by the 2-His-1-Carboxylate Facial Triad
  52. Reduction of Urease Activity by Interaction with the Flap Covering the Active Site
  53. Homology modeling, molecular dynamics, and site-directed mutagenesis study of AlkB human homolog 1 (ALKBH1)
  54. Sustained photobiological hydrogen production in the presence of N2 by nitrogenase mutants of the heterocyst-forming cyanobacterium Anabaena
  55. Mechanism of the 6-Hydroxy-3-succinoyl-pyridine 3-Monooxygenase Flavoprotein from Pseudomonas putida S16
  56. Nickel-dependent metalloenzymes
  57. Measuring the Orientation of Taurine in the Active Site of the Non-Heme Fe(II)/α-Ketoglutarate-Dependent Taurine Hydroxylase (TauD) Using Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy
  58. Analysis of a Soluble (UreD:UreF:UreG)2 Accessory Protein Complex and Its Interactions with Klebsiella aerogenes Urease by Mass Spectrometry
  59. ALKBH1 Is Dispensable for Abasic Site Cleavage during Base Excision Repair and Class Switch Recombination
  60. A covalent protein–DNA 5′-product adduct is generated following AP lyase activity of human ALKBH1 (AlkB homologue 1)
  61. Biosynthesis of the Urease Metallocenter
  62. Nickel-Binding Sites in Proteins
  63. Apoprotein isolation and activation, and vibrational structure of the Helicobacter mustelae iron urease
  64. Characterization of a Trypanosoma brucei Alkb homolog capable of repairing alkylated DNA
  65. Klebsiella aerogenes UreF: Identification of the UreG Binding Site and Role in Enhancing the Fidelity of Urease Activation
  66. Genetic Engineering of Cyanobacteria to Enhance Biohydrogen Production from Sunlight and Water
  67. Fructose-1,6-bisphosphate aldolase (class II) is the primary site of nickel toxicity in Escherichia coli
  68. Function of UreB in Klebsiella aerogenes Urease
  69. The Escherichia coli alkylation response protein AidB is a redox partner of flavodoxin and binds RNA and acyl carrier protein
  70. Iron-containing urease in a pathogenic bacterium
  71. Mechanisms of nickel toxicity in microorganisms
  72. Crystal structure of a truncated urease accessory protein UreF from Helicobacter pylori
  73. Site-Directed Mutagenesis of the Anabaena sp. Strain PCC 7120 Nitrogenase Active Site To Increase Photobiological Hydrogen Production
  74. Mutagenesis of Klebsiella aerogenes UreG To Probe Nickel Binding and Interactions with Other Urease-Related Proteins
  75. Metal and substrate binding to an Fe(II) dioxygenase resolved by UV spectroscopy with global regression analysis
  76. Trypanosoma brucei brucei: Thymine 7-hydroxylase-like proteins
  77. Characterization of the Klebsiella aerogenes Urease Accessory Protein UreD in Fusion with the Maltose Binding Protein
  78. Insight into the mechanism of an iron dioxygenase by resolution of steps following the Fe IV ═O species
  79. Human AlkB homologue 1 (ABH1) exhibits DNA lyase activity at abasic sites
  80. Interplay of metal ions and urease
  81. The structure of urease activation complexes examined by flexibility analysis, mutagenesis, and small-angle X-ray scattering
  82. Identification of Escherichia coli YgaF as an l-2-Hydroxyglutarate Oxidase
  83. Characterization of active site variants of xanthine hydroxylase from Aspergillus nidulans
  84. FeII/α-ketoglutarate hydroxylases involved in nucleobase, nucleoside, nucleotide, and chromatin metabolism
  85. CrII Reactivity of Taurine/α-Ketoglutarate Dioxygenase
  86. Thermodynamics of Ni2+, Cu2+, and Zn2+ Binding to the Urease Metallochaperone UreE
  87. Metal ligand substitution and evidence for quinone formation in taurine/α-ketoglutarate dioxygenase
  88. Probing the Iron−Substrate Orientation for Taurine/α-Ketoglutarate Dioxygenase Using Deuterium Electron Spin Echo Envelope Modulation Spectroscopy
  89. Purification and Characterization of the FeII- and α-Ketoglutarate-Dependent Xanthine Hydroxylase from Aspergillus nidulans
  90. The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase
  91. New Insights into Acetone Metabolism▿
  92. The UreEF Fusion Protein Provides a Soluble and Functional Form of the UreF Urease Accessory Protein
  93. Inhibition of urease by bismuth(III): Implications for the mechanism of action of bismuth drugs
  94. An assay for Fe(II)/2-oxoglutarate-dependent dioxygenases by enzyme-coupled detection of succinate formation
  95. Structural basis for the enantiospecificities ofR- andS-specific phenoxypropionate/α-ketoglutarate dioxygenases
  96. The AidB Component of the Escherichia coli Adaptive Response to Alkylating Agents Is a Flavin-Containing, DNA-Binding Protein
  97. Self-hydroxylation of taurine/α-ketoglutarate dioxygenase: evidence for more than one oxygen activation mechanism
  98. Kinetic and spectroscopic investigation of CoII, NiII, and N-oxalylglycine inhibition of the FeII/α-ketoglutarate dioxygenase, TauD
  99. Biosynthesis of Active Bacillus subtilis Urease in the Absence of Known Urease Accessory Proteins
  100. Purification and Properties of the Klebsiella aerogenes UreE Metal-Binding Domain, a Functional Metallochaperone of Urease
  101. Steady-State and Transient Kinetic Analyses of Taurine/α-Ketoglutarate Dioxygenase:  Effects of Oxygen Concentration, Alternative Sulfonates, and Active-Site Variants on the FeIV-oxo Intermediate
  102. Biosynthesis of Metal Sites
  103. Metabolic Versatility of Prokaryotes for Urea Decomposition
  104. Aberrant activity of the DNA repair enzyme AlkB
  105. Chemical Cross-linking and Mass Spectrometric Identification of Sites of Interaction for UreD, UreF, and Urease
  106. Direct Detection of Oxygen Intermediates in the Non-Heme Fe Enzyme Taurine/α-Ketoglutarate Dioxygenase
  107. Fe(II)/α-Ketoglutarate-Dependent Hydroxylases and Related Enzymes
  108. Biosynthesis of Metal Sites
  109. Nickel uptake and utilization by microorganisms
  110. Ni and CO: more surprises
  111. Interconversion of two oxidized forms of taurine/α-ketoglutarate dioxygenase, a non-heme iron hydroxylase: Evidence for bicarbonate binding
  112. O2- and α-Ketoglutarate-Dependent Tyrosyl Radical Formation in TauD, an α-Keto Acid-Dependent Non-Heme Iron Dioxygenase
  113. Metal Ion Dependence of Recombinant Escherichia coli Allantoinase
  114. Intrinsic tryptophan fluorescence as a probe of metal and α-ketoglutarate binding to TfdA, a mononuclear non-heme iron dioxygenase
  115. Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage
  116. Probing the 2,4-Dichlorophenoxyacetate/α-Ketoglutarate Dioxygenase Substrate-Binding Site by Site-Directed Mutagenesis and Mechanism-Based Inactivation
  117. tfdA-Like Genes in 2,4-Dichlorophenoxyacetic Acid-Degrading Bacteria Belonging to the Bradyrhizobium-Agromonas-Nitrobacter-Afipia Cluster in α-Proteobacteria
  118. Non-heme iron oxygenases
  119. X-ray Crystal Structure of Escherichia coli Taurine/α-Ketoglutarate Dioxygenase Complexed to Ferrous Iron and Substrates,
  120. Crystal Structure of Klebsiella aerogenesUreE, a Nickel-binding Metallochaperone for Urease Activation
  121. Dual Effects of Ionic Strength on Klebsiella aerogenes Urease: pH-Dependent Activation and Inhibition
  122. Alternative Reactivity of an α-Ketoglutarate-Dependent Iron(II) Oxygenase:  Enzyme Self-Hydroxylation
  123. Resonance Raman Studies of the Iron(II)−α-Keto Acid Chromophore in Model and Enzyme Complexes
  124. UreE Stimulation of GTP-Dependent Urease Activation in the UreD-UreF-UreG-urease Apoprotein Complex
  125. Kinetic and Structural Characterization of Urease Active Site Variants,
  126. Fluoride Inhibition of Klebsiella aerogenes Urease:  Mechanistic Implications of a Pseudo-uncompetitive, Slow-Binding Inhibitor
  127. In Vivo and in Vitro Kinetics of Metal Transfer by the Klebsiella aerogenes Urease Nickel Metallochaperone, UreE
  128. Site-directed Mutagenesis of 2,4-Dichlorophenoxyacetic Acid/α-Ketoglutarate Dioxygenase
  129. Herbicide-Degrading α-Keto Acid-Dependent Enzyme TfdA:  Metal Coordination Environment and Mechanistic Insights
  130. Stopped-Flow Kinetic Analysis ofEscherichia coliTaurine/α-Ketoglutarate Dioxygenase:  Interactions with α-Ketoglutarate, Taurine, and Oxygen
  131. GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins
  132. Characterization of metal-substituted Klebsiella aerogenes urease
  133. Assays for Allantoinase
  134. Identification of Metal-Binding Residues in the Klebsiella aerogenes Urease Nickel Metallochaperone, UreE
  135. X-ray absorption spectroscopic analysis of Fe(II) and Cu(II) forms of a herbicide-degrading α-ketoglutarate dioxygenase
  136. Chemical Rescue of Klebsiella aerogenes Urease Variants Lacking the Carbamylated-Lysine Nickel Ligand,
  137. Ascorbic Acid-Dependent Turnover and Reactivation of 2,4-Dichlorophenoxyacetic Acid/α-Ketoglutarate Dioxygenase Using Thiophenoxyacetic Acid
  138. Substitution of the Urease Active Site Carbamate by Dithiocarbamate and Vanadate
  139. Distribution of the tfdA Gene in Soil Bacteria That Do Not Degrade 2,4-Dichlorophenoxyacetic Acid (2,4-D)
  140. Structures of Cys319 Variants and Acetohydroxamate-Inhibited Klebsiella aerogenes Urease,
  141. Characterization of the Mononickel Metallocenter in H134A Mutant Urease
  142. Metal Ion Interactions with Urease and UreD-Urease Apoproteins
  143. Urease activity in the crystalline state
  144. Characterization of the first enzyme in 2,4-dichlorophenoxyacetic acid metabolism.
  145. The crystal structure of urease from Klebsiella aerogenes
  146. Requirement of Carbon Dioxide for in Vitro Assembly of the Urease Nickel Metallocenter
  147. Nickel enzymes in microbes
  148. In vitro activation of urease apoprotein and role of UreD as a chaperone required for nickel metallocenter assembly.
  149. Purification and characterization ofKlebsiella aerogenesUreE protein: A nickel-binding protein that functions in urease metallocenter assembly
  150. Site-directed mutagenesis ofKlebsiella aerogenesurease: Identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis
  151. Diethylpyrocarbonate reactivity ofKlebsiella aerogenes urease: Effect ofpH and active site ligands on the rate of inactivation
  152. Biochemistry of Nickel
  153. Hydrogenase
  154. Methyl Coenzyme M Reductase
  155. Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes
  156. Characterization of urease from Sporosarcina ureae
  157. [33] Separation of flavins and flavin analogs by high-performance liquid chromatography
  158. Factor 390 chromophores: phosphodiester between AMP or GMP and methanogenic factor 420
  159. Paramagnetic centers in the nickel-containing, deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum
  160. Comparison of the iron proteins from the nitrogen fixation complexes of Azotobacter vinelandii, Clostridium pasteurianum , and Klebsiella pneumoniae