All Stories

  1. SpyTag-Enabled Assembly of Bacterial Microcompartment Trimers into Macroscopic Layered Protein Materials
  2. Quantifying selective metabolite transport for the bacterial microcompartment from Haliangium ochraceum with molecular dynamics simulations
  3. Quantifying Selective Metabolite Transport for the Bacterial Microcompartment from Haliangium ochraceum with Molecular Dynamics Simulations
  4. Ancestral Sequence Reconstruction of the Ethylene-Forming Enzyme
  5. Sumner’s legacy: a century of urease crystals and recent structural advances
  6. Biochemical, Structural, and Conformational Characterization of a Fungal Ethylene-Forming Enzyme
  7. Overcoming barriers for investigating nickel-pincer nucleotide cofactor-related enzymes
  8. Structural Basis for the Catalysis and Substrate Specificity of a LarA Racemase with a Broad Substrate Spectrum
  9. A structural view of nickel-pincer nucleotide cofactor-related biochemistry
  10. Structural, Spectroscopic, and Computational Insights from Canavanine-Bound and Two Catalytically Compromised Variants of the Ethylene-Forming Enzyme
  11. Bile salt hydrolase acyltransferase activity expands bile acid diversity
  12. Biosynthesis and Functions of the Nickel-Pincer Nucleotide (NPN) Cofactor
  13. Structure of the LarB–Substrate Complex and Identification of a Reaction Intermediate during Nickel-Pincer Nucleotide Cofactor Biosynthesis
  14. Dioxygen Binding Is Controlled by the Protein Environment in Non‐heme FeII and 2‐Oxoglutarate Oxygenases: A Study on Histone Demethylase PHF8 and an Ethylene‐Forming Enzyme
  15. Irreversible Inactivation of Lactate Racemase by Sodium Borohydride Reveals Reactivity of the Nickel–Pincer Nucleotide Cofactor
  16. Biological formation of ethylene
  17. Can an external electric field switch between ethylene formation and l-arginine hydroxylation in the ethylene forming enzyme?
  18. The nickel-pincer coenzyme of lactate racemase: A case study of uncovering cofactor structure and biosynthesis
  19. Five decades of metalloenzymology
  20. Sulfur incorporation into biomolecules: recent advances
  21. Bile salt hydrolase/aminoacyltransferase shapes the microbiome
  22. Unveiling the mechanisms and biosynthesis of a novel nickel-pincer enzyme
  23. Characterization of a [4Fe-4S]-dependent LarE sulfur insertase that facilitates nickel-pincer nucleotide cofactor biosynthesis in Thermotoga maritima
  24. Characterization of the nickel-inserting cyclometallase LarC from Moorella thermoacetica and identification of a cytidinylylated reaction intermediate
  25. Structural and mutational characterization of a malate racemase from the LarA superfamily
  26. Iron-containing ureases
  27. The LarB carboxylase/hydrolase forms a transient cysteinyl-pyridine intermediate during nickel-pincer nucleotide cofactor biosynthesis
  28. Atomic and Electronic Structure Determinants Distinguish between Ethylene Formation and l-Arginine Hydroxylation Reaction Mechanisms in the Ethylene-Forming Enzyme
  29. 1H-HYSCORE Reveals Structural Details at the Fe(II) Active Site of Taurine:2-Oxoglutarate Dioxygenase
  30. Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases
  31. Biological Pincer Complexes
  32. Lanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function
  33. Crystallographic characterization of a tri-Asp metal-binding site at the three-fold symmetry axis of LarE
  34. Nickel-Pincer Nucleotide Cofactor-Containing Enzymes
  35. New metal cofactors and recent metallocofactor insights
  36. Structural Origin of the Large Redox-Linked Reorganization in the 2-Oxoglutarate Dependent Oxygenase, TauD
  37. Strongly Coupled Redox-Linked Conformational Switching at the Active Site of the Non-Heme Iron-Dependent Dioxygenase, TauD
  38. A structural perspective on the PP-loop ATP pyrophosphatase family
  39. Thermodynamics of Iron(II) and Substrate Binding to the Ethylene-Forming Enzyme
  40. Analysis of the Active Site Cysteine Residue of the Sacrificial Sulfur Insertase LarE from Lactobacillus plantarum
  41. Biosynthesis of the nickel-pincer nucleotide cofactor of lactate racemase requires a CTP-dependent cyclometallase
  42. Lactate Racemase Nickel-Pincer Cofactor Operates by a Proton-Coupled Hydride Transfer Mechanism
  43. Characterization of human AlkB homolog 1 produced in mammalian cells and demonstration of mitochondrial dysfunction in ALKBH1-deficient cells
  44. Structures and Mechanisms of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme: Substrate Binding Creates a Twist
  45. Structural insights into the catalytic mechanism of a sacrificial sulfur insertase of the N-type ATP pyrophosphatase family, LarE
  46. Correction to Biochemical and Spectroscopic Characterization of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme from Pseudomonas syringae pv. phaseolicola PK2
  47. Biochemical Characterization of AP Lyase and m6A Demethylase Activities of Human AlkB Homologue 1 (ALKBH1)
  48. ALKBH7 Variant Related to Prostate Cancer Exhibits Altered Substrate Binding
  49. Nickel-pincer cofactor biosynthesis involves LarB-catalyzed pyridinium carboxylation and LarE-dependent sacrificial sulfur insertion
  50. Mutational and Computational Evidence That a Nickel-Transfer Tunnel in UreD Is Used for Activation of Klebsiella aerogenes Urease
  51. Catalytic Mechanisms of Fe(II)- and 2-Oxoglutarate-dependent Oxygenases
  52. A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase
  53. Calorimetric Assessment of Fe2+ Binding to α-Ketoglutarate/Taurine Dioxygenase: Ironing Out the Energetics of Metal Coordination by the 2-His-1-Carboxylate Facial Triad
  54. Reduction of Urease Activity by Interaction with the Flap Covering the Active Site
  55. Homology modeling, molecular dynamics, and site-directed mutagenesis study of AlkB human homolog 1 (ALKBH1)
  56. Sustained photobiological hydrogen production in the presence of N2 by nitrogenase mutants of the heterocyst-forming cyanobacterium Anabaena
  57. Mechanism of the 6-Hydroxy-3-succinoyl-pyridine 3-Monooxygenase Flavoprotein from Pseudomonas putida S16
  58. Nickel-dependent metalloenzymes
  59. Measuring the Orientation of Taurine in the Active Site of the Non-Heme Fe(II)/α-Ketoglutarate-Dependent Taurine Hydroxylase (TauD) Using Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy
  60. Analysis of a Soluble (UreD:UreF:UreG)2 Accessory Protein Complex and Its Interactions with Klebsiella aerogenes Urease by Mass Spectrometry
  61. ALKBH1 Is Dispensable for Abasic Site Cleavage during Base Excision Repair and Class Switch Recombination
  62. A covalent protein–DNA 5′-product adduct is generated following AP lyase activity of human ALKBH1 (AlkB homologue 1)
  63. Biosynthesis of the Urease Metallocenter
  64. Nickel-Binding Sites in Proteins
  65. Apoprotein isolation and activation, and vibrational structure of the Helicobacter mustelae iron urease
  66. Characterization of a Trypanosoma brucei Alkb homolog capable of repairing alkylated DNA
  67. Klebsiella aerogenes UreF: Identification of the UreG Binding Site and Role in Enhancing the Fidelity of Urease Activation
  68. Genetic Engineering of Cyanobacteria to Enhance Biohydrogen Production from Sunlight and Water
  69. Fructose-1,6-bisphosphate aldolase (class II) is the primary site of nickel toxicity in Escherichia coli
  70. Function of UreB in Klebsiella aerogenes Urease
  71. The Escherichia coli alkylation response protein AidB is a redox partner of flavodoxin and binds RNA and acyl carrier protein
  72. Iron-containing urease in a pathogenic bacterium
  73. Mechanisms of nickel toxicity in microorganisms
  74. Crystal structure of a truncated urease accessory protein UreF from Helicobacter pylori
  75. Site-Directed Mutagenesis of the Anabaena sp. Strain PCC 7120 Nitrogenase Active Site To Increase Photobiological Hydrogen Production
  76. Mutagenesis of Klebsiella aerogenes UreG To Probe Nickel Binding and Interactions with Other Urease-Related Proteins
  77. Metal and substrate binding to an Fe(II) dioxygenase resolved by UV spectroscopy with global regression analysis
  78. Trypanosoma brucei brucei: Thymine 7-hydroxylase-like proteins
  79. Characterization of the Klebsiella aerogenes Urease Accessory Protein UreD in Fusion with the Maltose Binding Protein
  80. Insight into the mechanism of an iron dioxygenase by resolution of steps following the Fe IV ═O species
  81. Human AlkB homologue 1 (ABH1) exhibits DNA lyase activity at abasic sites
  82. Interplay of metal ions and urease
  83. The structure of urease activation complexes examined by flexibility analysis, mutagenesis, and small-angle X-ray scattering
  84. Identification of Escherichia coli YgaF as an l-2-Hydroxyglutarate Oxidase
  85. Characterization of active site variants of xanthine hydroxylase from Aspergillus nidulans
  86. FeII/α-ketoglutarate hydroxylases involved in nucleobase, nucleoside, nucleotide, and chromatin metabolism
  87. CrII Reactivity of Taurine/α-Ketoglutarate Dioxygenase
  88. Thermodynamics of Ni2+, Cu2+, and Zn2+ Binding to the Urease Metallochaperone UreE
  89. Metal ligand substitution and evidence for quinone formation in taurine/α-ketoglutarate dioxygenase
  90. Probing the Iron−Substrate Orientation for Taurine/α-Ketoglutarate Dioxygenase Using Deuterium Electron Spin Echo Envelope Modulation Spectroscopy
  91. Purification and Characterization of the FeII- and α-Ketoglutarate-Dependent Xanthine Hydroxylase from Aspergillus nidulans
  92. The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase
  93. New Insights into Acetone Metabolism▿
  94. The UreEF Fusion Protein Provides a Soluble and Functional Form of the UreF Urease Accessory Protein
  95. Inhibition of urease by bismuth(III): Implications for the mechanism of action of bismuth drugs
  96. An assay for Fe(II)/2-oxoglutarate-dependent dioxygenases by enzyme-coupled detection of succinate formation
  97. Structural basis for the enantiospecificities ofR- andS-specific phenoxypropionate/α-ketoglutarate dioxygenases
  98. The AidB Component of the Escherichia coli Adaptive Response to Alkylating Agents Is a Flavin-Containing, DNA-Binding Protein
  99. Self-hydroxylation of taurine/α-ketoglutarate dioxygenase: evidence for more than one oxygen activation mechanism
  100. Kinetic and spectroscopic investigation of CoII, NiII, and N-oxalylglycine inhibition of the FeII/α-ketoglutarate dioxygenase, TauD
  101. Biosynthesis of Active Bacillus subtilis Urease in the Absence of Known Urease Accessory Proteins
  102. Purification and Properties of the Klebsiella aerogenes UreE Metal-Binding Domain, a Functional Metallochaperone of Urease
  103. Steady-State and Transient Kinetic Analyses of Taurine/α-Ketoglutarate Dioxygenase:  Effects of Oxygen Concentration, Alternative Sulfonates, and Active-Site Variants on the FeIV-oxo Intermediate
  104. Biosynthesis of Metal Sites
  105. Metabolic Versatility of Prokaryotes for Urea Decomposition
  106. Aberrant activity of the DNA repair enzyme AlkB
  107. Chemical Cross-linking and Mass Spectrometric Identification of Sites of Interaction for UreD, UreF, and Urease
  108. Direct Detection of Oxygen Intermediates in the Non-Heme Fe Enzyme Taurine/α-Ketoglutarate Dioxygenase
  109. Fe(II)/α-Ketoglutarate-Dependent Hydroxylases and Related Enzymes
  110. Biosynthesis of Metal Sites
  111. Nickel uptake and utilization by microorganisms
  112. Ni and CO: more surprises
  113. Interconversion of two oxidized forms of taurine/α-ketoglutarate dioxygenase, a non-heme iron hydroxylase: Evidence for bicarbonate binding
  114. O2- and α-Ketoglutarate-Dependent Tyrosyl Radical Formation in TauD, an α-Keto Acid-Dependent Non-Heme Iron Dioxygenase
  115. Metal Ion Dependence of Recombinant Escherichia coli Allantoinase
  116. Intrinsic tryptophan fluorescence as a probe of metal and α-ketoglutarate binding to TfdA, a mononuclear non-heme iron dioxygenase
  117. Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage
  118. Probing the 2,4-Dichlorophenoxyacetate/α-Ketoglutarate Dioxygenase Substrate-Binding Site by Site-Directed Mutagenesis and Mechanism-Based Inactivation
  119. tfdA-Like Genes in 2,4-Dichlorophenoxyacetic Acid-Degrading Bacteria Belonging to the Bradyrhizobium-Agromonas-Nitrobacter-Afipia Cluster in α-Proteobacteria
  120. Non-heme iron oxygenases
  121. X-ray Crystal Structure of Escherichia coli Taurine/α-Ketoglutarate Dioxygenase Complexed to Ferrous Iron and Substrates,
  122. Crystal Structure of Klebsiella aerogenesUreE, a Nickel-binding Metallochaperone for Urease Activation
  123. Dual Effects of Ionic Strength on Klebsiella aerogenes Urease: pH-Dependent Activation and Inhibition
  124. Alternative Reactivity of an α-Ketoglutarate-Dependent Iron(II) Oxygenase:  Enzyme Self-Hydroxylation
  125. Resonance Raman Studies of the Iron(II)−α-Keto Acid Chromophore in Model and Enzyme Complexes
  126. UreE Stimulation of GTP-Dependent Urease Activation in the UreD-UreF-UreG-urease Apoprotein Complex
  127. Kinetic and Structural Characterization of Urease Active Site Variants,
  128. Fluoride Inhibition of Klebsiella aerogenes Urease:  Mechanistic Implications of a Pseudo-uncompetitive, Slow-Binding Inhibitor
  129. In Vivo and in Vitro Kinetics of Metal Transfer by the Klebsiella aerogenes Urease Nickel Metallochaperone, UreE
  130. Site-directed Mutagenesis of 2,4-Dichlorophenoxyacetic Acid/α-Ketoglutarate Dioxygenase
  131. Herbicide-Degrading α-Keto Acid-Dependent Enzyme TfdA:  Metal Coordination Environment and Mechanistic Insights
  132. Stopped-Flow Kinetic Analysis ofEscherichia coliTaurine/α-Ketoglutarate Dioxygenase:  Interactions with α-Ketoglutarate, Taurine, and Oxygen
  133. GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins
  134. Characterization of metal-substituted Klebsiella aerogenes urease
  135. Assays for Allantoinase
  136. Identification of Metal-Binding Residues in the Klebsiella aerogenes Urease Nickel Metallochaperone, UreE
  137. X-ray absorption spectroscopic analysis of Fe(II) and Cu(II) forms of a herbicide-degrading α-ketoglutarate dioxygenase
  138. Chemical Rescue of Klebsiella aerogenes Urease Variants Lacking the Carbamylated-Lysine Nickel Ligand,
  139. Ascorbic Acid-Dependent Turnover and Reactivation of 2,4-Dichlorophenoxyacetic Acid/α-Ketoglutarate Dioxygenase Using Thiophenoxyacetic Acid
  140. Substitution of the Urease Active Site Carbamate by Dithiocarbamate and Vanadate
  141. Distribution of the tfdA Gene in Soil Bacteria That Do Not Degrade 2,4-Dichlorophenoxyacetic Acid (2,4-D)
  142. Structures of Cys319 Variants and Acetohydroxamate-Inhibited Klebsiella aerogenes Urease,
  143. Characterization of the Mononickel Metallocenter in H134A Mutant Urease
  144. Metal Ion Interactions with Urease and UreD-Urease Apoproteins
  145. Urease activity in the crystalline state
  146. Characterization of the first enzyme in 2,4-dichlorophenoxyacetic acid metabolism.
  147. The crystal structure of urease from Klebsiella aerogenes
  148. Requirement of Carbon Dioxide for in Vitro Assembly of the Urease Nickel Metallocenter
  149. Nickel enzymes in microbes
  150. In vitro activation of urease apoprotein and role of UreD as a chaperone required for nickel metallocenter assembly.
  151. Purification and characterization ofKlebsiella aerogenesUreE protein: A nickel-binding protein that functions in urease metallocenter assembly
  152. Site-directed mutagenesis ofKlebsiella aerogenesurease: Identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis
  153. Diethylpyrocarbonate reactivity ofKlebsiella aerogenes urease: Effect ofpH and active site ligands on the rate of inactivation
  154. Biochemistry of Nickel
  155. Hydrogenase
  156. Methyl Coenzyme M Reductase
  157. Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes
  158. Characterization of urease from Sporosarcina ureae
  159. [33] Separation of flavins and flavin analogs by high-performance liquid chromatography
  160. Factor 390 chromophores: phosphodiester between AMP or GMP and methanogenic factor 420
  161. Paramagnetic centers in the nickel-containing, deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum
  162. Comparison of the iron proteins from the nitrogen fixation complexes of Azotobacter vinelandii, Clostridium pasteurianum , and Klebsiella pneumoniae