All Stories

  1. Quantifying selective metabolite transport for the bacterial microcompartment from Haliangium ochraceum with molecular dynamics simulations
  2. Quantifying Selective Metabolite Transport for the Bacterial Microcompartment from Haliangium ochraceum with Molecular Dynamics Simulations
  3. Ancestral Sequence Reconstruction of the Ethylene-Forming Enzyme
  4. Sumner’s legacy: a century of urease crystals and recent structural advances
  5. Biochemical, Structural, and Conformational Characterization of a Fungal Ethylene-Forming Enzyme
  6. Overcoming barriers for investigating nickel-pincer nucleotide cofactor-related enzymes
  7. Structural Basis for the Catalysis and Substrate Specificity of a LarA Racemase with a Broad Substrate Spectrum
  8. A structural view of nickel-pincer nucleotide cofactor-related biochemistry
  9. Structural, Spectroscopic, and Computational Insights from Canavanine-Bound and Two Catalytically Compromised Variants of the Ethylene-Forming Enzyme
  10. Bile salt hydrolase acyltransferase activity expands bile acid diversity
  11. Biosynthesis and Functions of the Nickel-Pincer Nucleotide (NPN) Cofactor
  12. Structure of the LarB–Substrate Complex and Identification of a Reaction Intermediate during Nickel-Pincer Nucleotide Cofactor Biosynthesis
  13. Dioxygen Binding Is Controlled by the Protein Environment in Non‐heme FeII and 2‐Oxoglutarate Oxygenases: A Study on Histone Demethylase PHF8 and an Ethylene‐Forming Enzyme
  14. Irreversible Inactivation of Lactate Racemase by Sodium Borohydride Reveals Reactivity of the Nickel–Pincer Nucleotide Cofactor
  15. Biological formation of ethylene
  16. Can an external electric field switch between ethylene formation and l-arginine hydroxylation in the ethylene forming enzyme?
  17. The nickel-pincer coenzyme of lactate racemase: A case study of uncovering cofactor structure and biosynthesis
  18. Five decades of metalloenzymology
  19. Sulfur incorporation into biomolecules: recent advances
  20. Bile salt hydrolase/aminoacyltransferase shapes the microbiome
  21. Unveiling the mechanisms and biosynthesis of a novel nickel-pincer enzyme
  22. Characterization of a [4Fe-4S]-dependent LarE sulfur insertase that facilitates nickel-pincer nucleotide cofactor biosynthesis in Thermotoga maritima
  23. Characterization of the nickel-inserting cyclometallase LarC from Moorella thermoacetica and identification of a cytidinylylated reaction intermediate
  24. Structural and mutational characterization of a malate racemase from the LarA superfamily
  25. Iron-containing ureases
  26. The LarB carboxylase/hydrolase forms a transient cysteinyl-pyridine intermediate during nickel-pincer nucleotide cofactor biosynthesis
  27. Atomic and Electronic Structure Determinants Distinguish between Ethylene Formation and l-Arginine Hydroxylation Reaction Mechanisms in the Ethylene-Forming Enzyme
  28. 1H-HYSCORE Reveals Structural Details at the Fe(II) Active Site of Taurine:2-Oxoglutarate Dioxygenase
  29. Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases
  30. Biological Pincer Complexes
  31. Lanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function
  32. Crystallographic characterization of a tri-Asp metal-binding site at the three-fold symmetry axis of LarE
  33. Nickel-Pincer Nucleotide Cofactor-Containing Enzymes
  34. New metal cofactors and recent metallocofactor insights
  35. Structural Origin of the Large Redox-Linked Reorganization in the 2-Oxoglutarate Dependent Oxygenase, TauD
  36. Strongly Coupled Redox-Linked Conformational Switching at the Active Site of the Non-Heme Iron-Dependent Dioxygenase, TauD
  37. A structural perspective on the PP-loop ATP pyrophosphatase family
  38. Thermodynamics of Iron(II) and Substrate Binding to the Ethylene-Forming Enzyme
  39. Analysis of the Active Site Cysteine Residue of the Sacrificial Sulfur Insertase LarE from Lactobacillus plantarum
  40. Biosynthesis of the nickel-pincer nucleotide cofactor of lactate racemase requires a CTP-dependent cyclometallase
  41. Lactate Racemase Nickel-Pincer Cofactor Operates by a Proton-Coupled Hydride Transfer Mechanism
  42. Characterization of human AlkB homolog 1 produced in mammalian cells and demonstration of mitochondrial dysfunction in ALKBH1-deficient cells
  43. Structures and Mechanisms of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme: Substrate Binding Creates a Twist
  44. Structural insights into the catalytic mechanism of a sacrificial sulfur insertase of the N-type ATP pyrophosphatase family, LarE
  45. Correction to Biochemical and Spectroscopic Characterization of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme from Pseudomonas syringae pv. phaseolicola PK2
  46. Biochemical Characterization of AP Lyase and m6A Demethylase Activities of Human AlkB Homologue 1 (ALKBH1)
  47. ALKBH7 Variant Related to Prostate Cancer Exhibits Altered Substrate Binding
  48. Nickel-pincer cofactor biosynthesis involves LarB-catalyzed pyridinium carboxylation and LarE-dependent sacrificial sulfur insertion
  49. Mutational and Computational Evidence That a Nickel-Transfer Tunnel in UreD Is Used for Activation of Klebsiella aerogenes Urease
  50. Catalytic Mechanisms of Fe(II)- and 2-Oxoglutarate-dependent Oxygenases
  51. A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase
  52. Calorimetric Assessment of Fe2+ Binding to α-Ketoglutarate/Taurine Dioxygenase: Ironing Out the Energetics of Metal Coordination by the 2-His-1-Carboxylate Facial Triad
  53. Reduction of Urease Activity by Interaction with the Flap Covering the Active Site
  54. Homology modeling, molecular dynamics, and site-directed mutagenesis study of AlkB human homolog 1 (ALKBH1)
  55. Sustained photobiological hydrogen production in the presence of N2 by nitrogenase mutants of the heterocyst-forming cyanobacterium Anabaena
  56. Mechanism of the 6-Hydroxy-3-succinoyl-pyridine 3-Monooxygenase Flavoprotein from Pseudomonas putida S16
  57. Nickel-dependent metalloenzymes
  58. Measuring the Orientation of Taurine in the Active Site of the Non-Heme Fe(II)/α-Ketoglutarate-Dependent Taurine Hydroxylase (TauD) Using Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy
  59. Analysis of a Soluble (UreD:UreF:UreG)2 Accessory Protein Complex and Its Interactions with Klebsiella aerogenes Urease by Mass Spectrometry
  60. ALKBH1 Is Dispensable for Abasic Site Cleavage during Base Excision Repair and Class Switch Recombination
  61. A covalent protein–DNA 5′-product adduct is generated following AP lyase activity of human ALKBH1 (AlkB homologue 1)
  62. Biosynthesis of the Urease Metallocenter
  63. Nickel-Binding Sites in Proteins
  64. Apoprotein isolation and activation, and vibrational structure of the Helicobacter mustelae iron urease
  65. Characterization of a Trypanosoma brucei Alkb homolog capable of repairing alkylated DNA
  66. Klebsiella aerogenes UreF: Identification of the UreG Binding Site and Role in Enhancing the Fidelity of Urease Activation
  67. Genetic Engineering of Cyanobacteria to Enhance Biohydrogen Production from Sunlight and Water
  68. Fructose-1,6-bisphosphate aldolase (class II) is the primary site of nickel toxicity in Escherichia coli
  69. Function of UreB in Klebsiella aerogenes Urease
  70. The Escherichia coli alkylation response protein AidB is a redox partner of flavodoxin and binds RNA and acyl carrier protein
  71. Iron-containing urease in a pathogenic bacterium
  72. Mechanisms of nickel toxicity in microorganisms
  73. Crystal structure of a truncated urease accessory protein UreF from Helicobacter pylori
  74. Site-Directed Mutagenesis of the Anabaena sp. Strain PCC 7120 Nitrogenase Active Site To Increase Photobiological Hydrogen Production
  75. Mutagenesis of Klebsiella aerogenes UreG To Probe Nickel Binding and Interactions with Other Urease-Related Proteins
  76. Metal and substrate binding to an Fe(II) dioxygenase resolved by UV spectroscopy with global regression analysis
  77. Trypanosoma brucei brucei: Thymine 7-hydroxylase-like proteins
  78. Characterization of the Klebsiella aerogenes Urease Accessory Protein UreD in Fusion with the Maltose Binding Protein
  79. Insight into the mechanism of an iron dioxygenase by resolution of steps following the Fe IV ═O species
  80. Human AlkB homologue 1 (ABH1) exhibits DNA lyase activity at abasic sites
  81. Interplay of metal ions and urease
  82. The structure of urease activation complexes examined by flexibility analysis, mutagenesis, and small-angle X-ray scattering
  83. Identification of Escherichia coli YgaF as an l-2-Hydroxyglutarate Oxidase
  84. Characterization of active site variants of xanthine hydroxylase from Aspergillus nidulans
  85. FeII/α-ketoglutarate hydroxylases involved in nucleobase, nucleoside, nucleotide, and chromatin metabolism
  86. CrII Reactivity of Taurine/α-Ketoglutarate Dioxygenase
  87. Thermodynamics of Ni2+, Cu2+, and Zn2+ Binding to the Urease Metallochaperone UreE
  88. Metal ligand substitution and evidence for quinone formation in taurine/α-ketoglutarate dioxygenase
  89. Probing the Iron−Substrate Orientation for Taurine/α-Ketoglutarate Dioxygenase Using Deuterium Electron Spin Echo Envelope Modulation Spectroscopy
  90. Purification and Characterization of the FeII- and α-Ketoglutarate-Dependent Xanthine Hydroxylase from Aspergillus nidulans
  91. The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase
  92. New Insights into Acetone Metabolism▿
  93. The UreEF Fusion Protein Provides a Soluble and Functional Form of the UreF Urease Accessory Protein
  94. Inhibition of urease by bismuth(III): Implications for the mechanism of action of bismuth drugs
  95. An assay for Fe(II)/2-oxoglutarate-dependent dioxygenases by enzyme-coupled detection of succinate formation
  96. Structural basis for the enantiospecificities ofR- andS-specific phenoxypropionate/α-ketoglutarate dioxygenases
  97. The AidB Component of the Escherichia coli Adaptive Response to Alkylating Agents Is a Flavin-Containing, DNA-Binding Protein
  98. Self-hydroxylation of taurine/α-ketoglutarate dioxygenase: evidence for more than one oxygen activation mechanism
  99. Kinetic and spectroscopic investigation of CoII, NiII, and N-oxalylglycine inhibition of the FeII/α-ketoglutarate dioxygenase, TauD
  100. Biosynthesis of Active Bacillus subtilis Urease in the Absence of Known Urease Accessory Proteins
  101. Purification and Properties of the Klebsiella aerogenes UreE Metal-Binding Domain, a Functional Metallochaperone of Urease
  102. Steady-State and Transient Kinetic Analyses of Taurine/α-Ketoglutarate Dioxygenase:  Effects of Oxygen Concentration, Alternative Sulfonates, and Active-Site Variants on the FeIV-oxo Intermediate
  103. Biosynthesis of Metal Sites
  104. Metabolic Versatility of Prokaryotes for Urea Decomposition
  105. Aberrant activity of the DNA repair enzyme AlkB
  106. Chemical Cross-linking and Mass Spectrometric Identification of Sites of Interaction for UreD, UreF, and Urease
  107. Direct Detection of Oxygen Intermediates in the Non-Heme Fe Enzyme Taurine/α-Ketoglutarate Dioxygenase
  108. Fe(II)/α-Ketoglutarate-Dependent Hydroxylases and Related Enzymes
  109. Biosynthesis of Metal Sites
  110. Nickel uptake and utilization by microorganisms
  111. Ni and CO: more surprises
  112. Interconversion of two oxidized forms of taurine/α-ketoglutarate dioxygenase, a non-heme iron hydroxylase: Evidence for bicarbonate binding
  113. O2- and α-Ketoglutarate-Dependent Tyrosyl Radical Formation in TauD, an α-Keto Acid-Dependent Non-Heme Iron Dioxygenase
  114. Metal Ion Dependence of Recombinant Escherichia coli Allantoinase
  115. Intrinsic tryptophan fluorescence as a probe of metal and α-ketoglutarate binding to TfdA, a mononuclear non-heme iron dioxygenase
  116. Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage
  117. Probing the 2,4-Dichlorophenoxyacetate/α-Ketoglutarate Dioxygenase Substrate-Binding Site by Site-Directed Mutagenesis and Mechanism-Based Inactivation
  118. tfdA-Like Genes in 2,4-Dichlorophenoxyacetic Acid-Degrading Bacteria Belonging to the Bradyrhizobium-Agromonas-Nitrobacter-Afipia Cluster in α-Proteobacteria
  119. Non-heme iron oxygenases
  120. X-ray Crystal Structure of Escherichia coli Taurine/α-Ketoglutarate Dioxygenase Complexed to Ferrous Iron and Substrates,
  121. Crystal Structure of Klebsiella aerogenesUreE, a Nickel-binding Metallochaperone for Urease Activation
  122. Dual Effects of Ionic Strength on Klebsiella aerogenes Urease: pH-Dependent Activation and Inhibition
  123. Alternative Reactivity of an α-Ketoglutarate-Dependent Iron(II) Oxygenase:  Enzyme Self-Hydroxylation
  124. Resonance Raman Studies of the Iron(II)−α-Keto Acid Chromophore in Model and Enzyme Complexes
  125. UreE Stimulation of GTP-Dependent Urease Activation in the UreD-UreF-UreG-urease Apoprotein Complex
  126. Kinetic and Structural Characterization of Urease Active Site Variants,
  127. Fluoride Inhibition of Klebsiella aerogenes Urease:  Mechanistic Implications of a Pseudo-uncompetitive, Slow-Binding Inhibitor
  128. In Vivo and in Vitro Kinetics of Metal Transfer by the Klebsiella aerogenes Urease Nickel Metallochaperone, UreE
  129. Site-directed Mutagenesis of 2,4-Dichlorophenoxyacetic Acid/α-Ketoglutarate Dioxygenase
  130. Herbicide-Degrading α-Keto Acid-Dependent Enzyme TfdA:  Metal Coordination Environment and Mechanistic Insights
  131. Stopped-Flow Kinetic Analysis ofEscherichia coliTaurine/α-Ketoglutarate Dioxygenase:  Interactions with α-Ketoglutarate, Taurine, and Oxygen
  132. GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins
  133. Characterization of metal-substituted Klebsiella aerogenes urease
  134. Assays for Allantoinase
  135. Identification of Metal-Binding Residues in the Klebsiella aerogenes Urease Nickel Metallochaperone, UreE
  136. X-ray absorption spectroscopic analysis of Fe(II) and Cu(II) forms of a herbicide-degrading α-ketoglutarate dioxygenase
  137. Chemical Rescue of Klebsiella aerogenes Urease Variants Lacking the Carbamylated-Lysine Nickel Ligand,
  138. Ascorbic Acid-Dependent Turnover and Reactivation of 2,4-Dichlorophenoxyacetic Acid/α-Ketoglutarate Dioxygenase Using Thiophenoxyacetic Acid
  139. Substitution of the Urease Active Site Carbamate by Dithiocarbamate and Vanadate
  140. Distribution of the tfdA Gene in Soil Bacteria That Do Not Degrade 2,4-Dichlorophenoxyacetic Acid (2,4-D)
  141. Structures of Cys319 Variants and Acetohydroxamate-Inhibited Klebsiella aerogenes Urease,
  142. Characterization of the Mononickel Metallocenter in H134A Mutant Urease
  143. Metal Ion Interactions with Urease and UreD-Urease Apoproteins
  144. Urease activity in the crystalline state
  145. Characterization of the first enzyme in 2,4-dichlorophenoxyacetic acid metabolism.
  146. The crystal structure of urease from Klebsiella aerogenes
  147. Requirement of Carbon Dioxide for in Vitro Assembly of the Urease Nickel Metallocenter
  148. Nickel enzymes in microbes
  149. In vitro activation of urease apoprotein and role of UreD as a chaperone required for nickel metallocenter assembly.
  150. Purification and characterization ofKlebsiella aerogenesUreE protein: A nickel-binding protein that functions in urease metallocenter assembly
  151. Site-directed mutagenesis ofKlebsiella aerogenesurease: Identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis
  152. Diethylpyrocarbonate reactivity ofKlebsiella aerogenes urease: Effect ofpH and active site ligands on the rate of inactivation
  153. Biochemistry of Nickel
  154. Hydrogenase
  155. Methyl Coenzyme M Reductase
  156. Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes
  157. Characterization of urease from Sporosarcina ureae
  158. [33] Separation of flavins and flavin analogs by high-performance liquid chromatography
  159. Factor 390 chromophores: phosphodiester between AMP or GMP and methanogenic factor 420
  160. Paramagnetic centers in the nickel-containing, deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum
  161. Comparison of the iron proteins from the nitrogen fixation complexes of Azotobacter vinelandii, Clostridium pasteurianum , and Klebsiella pneumoniae