What is it about?
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MSI) of tissue sections as well as the extraction of tissue homogenates, blood plasma and dried blood spots (DBS) coupled with LC–MS were employed to monitor the pharmacokinetics of metformin in mice.
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Why is it important?
MSI provided valuable information on the spatial distribution and relative concentration of metformin within tissue sections. The analysis of the extracts of tissue homogenates, blood plasma and blood spots provided quantitative data on metformin. The dried blood spot approach is a progressive method of sampling, especially in studies where the amount of available blood is limited.
Perspectives
MSI is a progressive analytical tool complementary to the conventional techniques used in pharmacokinetic studies. Due to its ability to provide unique information on the spatial distribution of the analyte within the given organ in time, a bright future can be envisaged for the application of MSI. Similarly, DBS sampling is progressive and provides more consistent data than the classical analysis of blood plasma via protein precipitation because repetitive blood collection from one animal is feasible with DBS sampling. Moreover, fewer animals are necessary for pharmacokinetic studies, thus rendering a significant additional benefit.
David Sykora
Vysoka skola chemicko-technologicka v Praze
Read the Original
This page is a summary of: Application of matrix-assisted laser desorption/ionization mass spectrometry imaging in combination with LC–MS in pharmacokinetic study of metformin, Bioanalysis, December 2017, Future Science,
DOI: 10.4155/bio-2017-0190.
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