What is it about?
The 'SINGLE-MOLECULE DEMON' (single-molecule ratchet) in IMAGING/MICROSCOPY/SUPER-RESOLUTION MICROSCOPY (NANOSCOPY) and SPECTROSCOPY: Dilute Liquids and Live Cells https://www.growkudos.com/publications/10.2174%252F138920111795470949/reader
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Why is it important?
Zeno Földes-Papp**: The FUNDAMENTAL NATURAL LAWS OF SINGLE-MOLECULE TIME RESOLUTION of freely diffusing molecules. ON THE MOVE TOWARDS NEW HORIZONS. A universal theory on single molecules was drawn upon experimental facts by forming the concept of "THE MEANINGFUL TIME Tm" acting AS FUNDAMENTAL NATURAL LAWS OF SINGLE-MOLECULE TIME RESOLUTION of freely diffusing molecules (i.e. with negligible external forces like a significant hydrodynamic flow or without firm binding or attachment (immobilization) to a solid phase like artificial surfaces and biological menbranes) in dilute liquid and live cells (3D-diffusional normal and anomalous processes as well as in or on artificial or biological membranes as (2D-diffusional normal and anomalous processes). The physical laws correspond to the MATHEMATICAL EQUATIONS which we use to describe every single molecule. This is the Theory of Single-Molecule Biophysics & Biochemistry Based On Individually Diffusing Molecules in Dilute Liquids and Live Cells. It must be made crystal clear that the underlying physics and molecular biochemistry can be modified easily but things hardly get worse when there is any trouble with the mathematics which rules the physical and biological formulations. WE PREVAILED AND YET OTHERS DID NOT. The advantages of the new 'Theory of Single-Molecule Biophysics & Biochemistry Based On Individually Diffusing Molecules in Dilute Liquids and Live Cells' are evident: The 'SINGLE-MOLECULE DEMON' (single-molecule ratchet) in IMAGING/MICROSCOPY/SUPER-RESOLUTION MICROSCOPY (NANOSCOPY) and SPECTROSCOPY: Dilute Liquids and Live Cells •https://www.growkudos.com/publications/10.2174%252F138920111795470949/reader •https://www.linkedin.com/pulse/how-get-high-single-molecule-biophysics-biochemistry-data-zeno?trk=public_profile_article_view
Perspectives
The thermodynamic Single-Molecule DEMON: How to avoid him in the measurements of dilute liquids and live cells without immobilization or flow: https://ajtm.journals.publicknowledgeproject.org/index.php/ajtm/article/view/1408 https://www.eurekaselect.com/article/124544
RETIRED - PRESERVE FROM BEING FORGOTTEN: Professor Zeno Földes-Papp [Biochemist, Gerontologist (Biochemiker, Geriater)]: Laying the Foundation of Single-Molecule Biophysics & Biochemistry Based On the Stochastic Nature of Diffusion: The Individual Molecule, from the Mathematical Core to the Physical Theory. -- I hope that my humble scientific work will be well received by the communities of single-molecule imaging and spectroscopy and by all users of these technologies as well as biotechnologies in the various and different disciplines:
Head of Geriatric Medicine (Medical Director of the Geriatric Service: Sektionsleitung Geriatrie) at Asklepios Klinikum Lindau (Bodensee), Bavaria, Germany
Read the Original
This page is a summary of: Individual Macromolecule Motion in a Crowded Living Cell, Current Pharmaceutical Biotechnology, January 2015, Bentham Science Publishers,
DOI: 10.2174/1389201016666141229103953.
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Resources
2014 Nobel Prize in Chemistry
Drs. Eric Betzig, Stefan W. Hell and William E. Moerner share the 2014 chemistry Nobel for the development of super-resolved fluorescence microscopy. “The Royal Swedish Academy of Sciences has decided to award the 2014 Nobel Prize in Chemistry to Dr. Eric Betzig at Howard Hughes Medical Institute, Ashburn, U.S.A.; Professor Stefan Hell at Max Planck Institute for Biophysical Chemistry, Göttingen, and the German Cancer Research Center, Heidelberg, Germany; and Professor William Moerner at Stanford University, Stanford, U.S.A., for the development of super-resolved fluorescence microscopy.” Much research effort was needed to realize that single-cell and single-molecule based approaches directly affect improvements in human health. However, many of these laboratory technologies are not available in a cheap and easy version. Single-molecule based fluorescence imaging and detection have the advantages of a small volume platform, digital analysis, elimination of processing steps, real-time measurements and automation of sample preparation. In 2015, Current Pharmaceutical Biotechnology will continue in this direction with quality papers that can be expected to trigger further investigations. Drs. Eric Betzig and William E. Moerner also developed methods and biotechniques which have enabled the study of single molecules in ongoing chemical reactions in living cells. Because now it is possible to see individual macromolecules moving about in a living cell, we can study chemistry at a single-molecule level and in real life. And this is very important to chemistry because chemistry has traditionally been about studying a large number of molecules and the effect that they have. The journal Current Pharmaceutical Biotechnology has published on this research and has been at the forefront for over more than one decade. Curr. Pharm. Biotechnol. has published thematic issues, original research and review articles about the entirely new possibilities for chemistry and for biochemistry by eminent scientists from around the world, some examples:
Original Research Article
"Single-molecule time resolution in dilute liquids and live cells at the molecular scale: Constraints on the measurement time" in American Journal of Translational Medicine 2021, 5 (3), 154-165.
Science news by Phys.org (USA): FEBRUARY 22, 2016
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Article
The thermodynamic signature of a single molecule or a single particle in dilute liquids and live cells: Single-Molecule Biophysics & Biochemistry based on the stochastic nature of diffusion: Instead of citing trendy and mediocre articles on studies "at the single-molecule level” in dilute liquids and live cells or on single molecules in diffusion processes, you may read insightful research articles on the quantiffication of thermodynamic jitter in the time domain (for example, the Brownian motion of molecules and particles) below: These dubious and far-fetched, i.e. non-serious and therefore pseudo-scientific, publications "at the single-molecule level” in dilute liquids and live cells without immobilization or significant hydrodynamic flow or on single molecules in diffusion processes will be replaced with that bright future. I think we can leave it at that. A head start is a proven theory, e.g. proven mathematically, that turns knowledge into strength, even at odds with the mediocre mainstream. What new insights can be gained from this? https://ajtm.journals.publicknowledgeproject.org/index.php/ajtm/article/view/2841/2537 COMPETENCE PAYS OFF!
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