Dispensing Processes Impact Apparent Biological Activity as Determined by Computational and Statistical Analyses

  • Sean Ekins, Joe Olechno, Antony J. Williams
  • PLoS ONE, May 2013, Public Library of Science (PLoS)
  • DOI: 10.1371/journal.pone.0062325

Dispensing processes profoundly influence estimates of biological activity of compounds

What is it about?

Dispensing processes profoundly influence estimates of biological activity of compounds. In this study using published inhibitor data for the tyrosine kinase EphB4, we show that IC50 values obtained via disposable tip-based serial dilution and dispensing versus acoustic dispensing differ by orders of magnitude with no correlation or ranking of datasets.

Why is it important?

Traditional dispensing processes are another important source of error in high-throughput screening that impacts computational and statistical analyses. These findings have far-reaching implications in biological research and calls into question the quality of HTS data available in the public databases and certainly emphasizes the need for appropriate meta data to be available to ensure that it is known WHAT dispensing method was used to deliver the material during the assay measurement


Dr Sean Ekins
Collaborations in Chemistry

When Joe first chatted with me about the patents he had found from AZ and what turned into this project, I had no idea what it would lead too. Its one of those cases were it not for the editorial / blog that brought us to his attention, this probably would never got done. Yes the dataset is limited, yes its only one target but still I think it proved a wake up call for everyone.

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The following have contributed to this page: Dr Antony John Williams, Dr Sean Ekins, and Dr Joe Olechno