What is it about?

Zinc is essential for skin health, immunological function, and cell development, and it may help prevent acne, inflammation, and other problems. A novel isocratic HPLC method for quantifying zinc in all types of supplements (tablets, capsules and injections) was developed using a carboxyl functional group packed column and a refractive index detector.

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Why is it important?

This study holds significant importance due to the limited availability of cost-effective published methodologies and insufficient ways for accurately detecting tiny amounts of zinc. The presented methodology is cost-effective and capable of quantifying trace levels of zinc.

Perspectives

Typically, the quantification of Zinc is accomplished by either EDTA titration or ion chromatography methods. The ion chromatography columns were transitioned to traditional high-performance liquid chromatography (HPLC) procedures with a refractive index detector. This article provides a comprehensive explanation of column chemistry as applied to the separation of zinc cations from anion salt peaks in a specific stationary phase within the column. To ascertain the stability-indicating characteristics, a comprehensive analysis involving forced degradation was conducted on all zinc supplements. The duration of the chromatographic run was 10 minutes, a timeframe that is deemed convenient for quality control laboratories. Moreover, it has met all the required regulatory requirements for utilization.

Mr. Kumarswamy Ummiti
Koneru Lakshmaiah Education Foundation

Read the Original

This page is a summary of: Development and Validation of a Method for Trace Level Zinc Quantification in Pharmaceutical Zinc Supplements using Carboxyl Functional Group Packed Column and Refractive Index Detector, Journal of AOAC International, September 2023, Oxford University Press (OUP),
DOI: 10.1093/jaoacint/qsad101.
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