CrystallineL-lysine with L-lysine enriched fermentation broth in broiler feed

What is it about?

L-lysine is a limiting amino acid in cereal grains and a certain amount is essentially required to supplement the feed for optimum growth of poultry and pigs. The study was conducted to test performance of commercial broiler chicks fed on a feed supplemented with indigenously produced crude form of L-lysine (fermentation broth). A mutant of local strain of Corynebacterium glutamicum was used as source bacterium for lysine production on cane molasses based production medium. A total of 2500 one-day-old broiler chicks (mixed sex) were allocated atrandom to five dietary treatments (500 chicks/treatment). Basal diet provided 8 g total lysine/kg feed (group A). Groups B and C (positive control) were supplemented with 2 and 3 g crystalline lysine-HCl/kg feed respectively, whereas groups D and E were supplemented with lysine enriched fermentation supernatant to provide similar amount of lysine to that of groups B and C. Least significant difference test revealed highly significant effect of two levels of fermentation product or crystalline lysine (HCl) on broiler performance. Significant improvement in feed conversion ratio (FCR) was observed in treatment groups.

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Why is it important?

Need of L-lysine (lysine) as an essential amino acid for mono-gastric species is well known. Commercial broiler chicks require 11 g lysine/kg feed from 0 to 3 weeks of age and 10 g/kg feed from 3 to 6 weeks of age (NRC 1994) to show optimum weight gain/growth. Poultry feed is formulated in such a way that available lysine from feed ingredients is calculated and formula requirement is then balanced through addition of crystalline lysine HCl. At least0.1% crystalline lysine (HCl) is included in broiler feed to meet formula requirements. This means a reasonable demand of liquid lysine in market can also be explored. The present study was designed to evaluate possibility of production of liquid lysine from indigenous mutant strain of C. glutamicum and comparative in-vivo efficacy of this liquid lysine on performance of commercial broiler chicks. Safety of the liquid product was confirmed through histological examination of viscera, levels of antibody titres and mortality ratio among treatment groups.

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