In vivo genotoxicity assessment of sunset yellow and sodium benzoate in female rats

Mohamed Yassin Ali, Gamal Mohamedin Hassan, Abdel Moniem Sadek Hassan, Zaher Ahmed Mohamed, Mohamed Fawzy Ramadan
  • Drug and Chemical Toxicology, September 2018, Taylor & Francis
  • DOI: 10.1080/01480545.2018.1510416

In vivo genotoxicity assessment of sunset yellow and sodium benzoate in female rats

What is it about?

The use of additives in different food products is growing up. It has attracted the attention towards the relation between the mutagenic potential of human diseases and food additives. Sunset yellow (SY) and sodium benzoate (NaB) are used as colorant and food additives worldwide. In the present study, genotoxic effects of different combinations of SY and NaB were assessed in vivo in female rats. Different combinations of SY and NaB were dissolved in water and administered daily to six animals groups for 12 weeks. Group 1 (control) received water, Group 2 received 5 mg/kg body weight (bw) SY plus 10 mg/kg bw NaB, group 3 received 5 mg/kg SY plus 100 mg/kg NaB, group 4 received 50 mg SY plus 100 mg/kg NaB, group 5 received 50 mg/kg SY plus 10 mg/kg NaB, group 6 received 200 mg/kg SY plus 750 mg/kg NaB, and group 7 received 20 mg/kg SY plus 75 mg/kg NaB.

Why is it important?

Genotoxicity investigations (Chromosomal aberration of bone marrow cells, Comet assay and DNA profile of liver cells) were carried out at the end of the experiment. Administration of 200 mg/kg SY plus 750 mg/kg NaB (group 6) induced the highest abnormalities percentage (1.5%) and showed structural abnormalities including end-to-end association, fragmentation, chromatid break, ring chromosome, and centric fusion break of chromosomes. Different combinations of SY and NaB induced an increase in the frequency of tailed nuclei (DNA damage) in liver cells. A concentration-dependent distinct DNA smear pattern was observed in the DNA isolated from liver cells of animals administered SY and NaB. In addition, administration of SY plus NaB resulted in an abnormal distribution of serum proteins. The results showed that the SY plus NaB could have genotoxic potential. With the increase applications of food additives, this study reported important data about screening the potential impacts.

Perspectives

Ph.D. candidate MOHAMED YASSIN YASSIN ALI
Shanghai Jiao Tong University

Although being approved for use in the food, combination of SY and NaB can pose considerable genotoxic effects on liver and bone marrow cells especially at higher doses. Controlling the consumption of these food additives is important for the health and limiting the use of SY and NaB, especially in foods used by children, is highly advisable

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http://dx.doi.org/10.1080/01480545.2018.1510416

The following have contributed to this page: Professor Mohamed Fawzy Ramadan Hassanien and Ph.D. candidate MOHAMED YASSIN YASSIN ALI