What is it about?

The temperature dynamics inside living cells play a critical role in maintaining the cells’ healthy status or driving the cells into unhealthy conditions. Here, we developed temperature-sensitive LysoDots and MitoDots to monitor the in situ temperature dynamics with specific organelles targetting strategies.

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Why is it important?

We demonstrated both LysoDots and MitoDots for simultaneously mapping the in situ lysosomal and mitochondrial thermal dynamics and thermal transitions in living cells with high sensitivity. Our observations indicate different metabolic pathways and thermal transitions between lysosomes and mitochondria.


The nano-thermometry probes provide a powerful tool for multi-modality functional imaging of subcellular organelles and interactions with high spatial, temporal and thermal dynamics resolutions.

Qian Peter Su
University of Technology Sydney

Read the Original

This page is a summary of: Spatiotemporally mapping temperature dynamics of lysosomes and mitochondria using cascade organelle-targeting upconversion nanoparticles, Proceedings of the National Academy of Sciences, November 2022, Proceedings of the National Academy of Sciences,
DOI: 10.1073/pnas.2207402119.
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