What is it about?

We showed that a consistent explanation of the experimental fluorescent spectra of Prodan and Laurdan in solution is obtained with the conclusive interpretation that the two bands observed are due to the emission from two independent states. Our results indicate that these are the n-π* state with small dipole moment at lower emission energy and the π-π* with large dipole moment at higher emission energy.

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Why is it important?

Prodan and Laurdan are fluorescent probes used to monitor biological systems such as lipid membrane, proteins and DNA. Understanding the origin of the dual uorescence and how the solvent or the biological system can affect the two independent emission bands can bring new insights about the molecular structure which the probe is monitoring.


We have clearly shown that the study of the first excited state alone is not enough to describe the complex dual fluorescence of Prodan, and Laurdan, in solution. This is even more complex in heterogeneous environment such as phospholipid membranes and other biological environments. However, we have not addressed the investigation of the internal conversions between the low-lying excited states of Prodan. I hope this article motivates new theoretical investigations of the corresponding internal conversions.

Kaline Coutinho
Universidade de Sao Paulo

Read the Original

This page is a summary of: A new interpretation of the absorption and the dual fluorescence of Prodan in solution, The Journal of Chemical Physics, December 2020, American Institute of Physics,
DOI: 10.1063/5.0025013.
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