Design of oligonucleotide-capped mesoporous silica nanoparticles for the detection of miRNA-145 by duplex and triplex formation

Àngela Ribes, Sara Santiago-Felipe, Anna Aviñó, Vicente Candela-Noguera, Ramón Eritja, Félix Sancenón, Ramón Martínez-Máñez, Elena Aznar
  • Sensors and Actuators B Chemical, December 2018, Elsevier
  • DOI: 10.1016/j.snb.2018.09.026

Detection of a microRNA involved in human disease

What is it about?

MicroRNAs regulates gene expression. Changes in the natural amounts of these regulators may lead to important diseases. We have used silica materials loaded with fluorescent compounds to visualize the presence of a biologically relevant microRNA and determine the amount of these microRNA in sera.

Why is it important?

The analysis of microRNA in human samples is of special interest for diagnosis. The relative large number of microRNA and the low amounts of these compounds difficult the precise analysis of microRNA. Methods based on PCR amplification are commonly used. The proposed method will provide a strong fluorescent signal that may avoid the use of PCR.

Perspectives

Ramon Eritja
CSIC

The manuscript provided an excellent opportunity to collaborate with materials scientists and confirm the utility of special triplex forming DNA probes developed by our group some time ago.

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http://dx.doi.org/10.1016/j.snb.2018.09.026

The following have contributed to this page: Ramon Eritja

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