What is it about?

We engineered the repDE ribosome-binding site of the pTRKH3 plasmid by site-directed mutagenesis to increase plasmid copy number in Lactococcus lactis LMG19460 cells. 215 copies of plasmid per chromosome were achieved, a 3.5-fold increase when compared to the nonmodified plasmid.

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Why is it important?

The generally recognized as safe Lactococcus lactis could constitute a safer alternative for plasmid production. A key requirement for the establishment of such a cost-effective manufacturing is the availability of high-copy number plasmids.

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This page is a summary of: Plasmid Copy Number of pTRKH3 in Lactococcus lactis is Increased by Modification of the repDE Ribosome‐Binding Site, Biotechnology Journal, May 2019, Wiley, DOI: 10.1002/biot.201800587.
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