All Stories

  1. Structure of the iminium reaction intermediate in an engineered aldolase explains the carboligation activity toward arylated ketones and aldehydes

    Article • Structure, September 2024, Elsevier

    Professor Mikael Widersten

  2. Structural enzymology studies with the substrate 3S‐hydroxybutanoyl‐CoA: bifunctional MFE1 is a less efficient dehydrogenase than monofunctional HAD

    Article • FEBS Open Bio, March 2024, Wiley

    rik wierenga, Professor Mikael Widersten

  3. High-throughput selection of (new) enzymes: phage display-mediated isolation of alkyl halide hydrolases from a library of active-site mutated epoxide hydrolases

    Article • Faraday Discussions, January 2024, Royal Society of Chemistry

    Professor Mikael Widersten

  4. Crystal structures and kinetic studies of a laboratory evolved aldehyde reductase explain the dramatic shift of its new substrate specificity

    Article • IUCrJ, June 2023, International Union of Crystallography

    rik wierenga, Professor Mikael Widersten

  5. Structures of lactaldehyde reductase, FucO, link enzyme activity to hydrogen bond networks and conformational dynamics

    Article • European Journal of Biochemistry, September 2022, Wiley

    Professor Mikael Widersten

  6. Facile synthesis of 2-hydroxyacetophenone from racemic styrene oxide catalyzed by engineered enzymes

    Article • Biotechnology Letters, June 2022, Springer Science + Business Media

    Professor Mikael Widersten

  7. Structure and Mechanism of a Cold-Adapted Bacterial Lipase

    Article • Biochemistry, May 2022, American Chemical Society (ACS)

    Professor Mikael Widersten

  8. The Role of Substrate-Coenzyme Crosstalk in Determining Turnover Rates in Rhodococcus ruber Alcohol Dehydrogenase

    Article • ACS Catalysis, July 2020, American Chemical Society (ACS)

    Shina Caroline Lynn Kamerlin, Professor Mikael Widersten

  9. Chemical and Biochemical Approaches for the Synthesis of Substituted Dihydroxybutanones and Di- and Tri-Hydroxypentanones

    Article • The Journal of Organic Chemistry, May 2019, American Chemical Society (ACS)

    Professor Mikael Widersten, Shina Caroline Lynn Kamerlin

  10. Chemical and Biochemical Approaches for the Synthesis of Substituted Dihydroxybutanones and Di-, and Tri-Hydroxypentanones

    Article • March 2019, American Chemical Society (ACS)

    Professor Mikael Widersten

  11. New Stereoselective Biocatalysts for Carboligation and Retro-Aldol Cleavage Reactions Derived from d-Fructose 6-Phosphate Aldolase

    Article • Biochemistry, September 2018, American Chemical Society (ACS)

    Professor Mikael Widersten

  12. Stereo- and Regioselectivity in Catalyzed Transformation of a 1,2-Disubstituted Vicinal Diol and the Corresponding Diketone by Wild Type and Laboratory Evolved Alcohol Dehydrogenases

    Article • ACS Catalysis, July 2018, American Chemical Society (ACS)

    Professor Mikael Widersten, Shina Caroline Lynn Kamerlin

  13. Epoxide hydrolysis as a model system for understanding flux through a branched reaction scheme

    Article • IUCrJ, March 2018, International Union of Crystallography

    Dr Shina Caroline Lynn Kamerlin, Professor Mikael Widersten, Shina Caroline Lynn Kamerlin

  14. Directed Evolution of Alcohol Dehydrogenase for Improved Stereoselective Redox Transformations of 1-Phenylethane-1,2-diol and Its Corresponding Acyloin

    Article • Biochemistry, February 2018, American Chemical Society (ACS)

    Professor Mikael Widersten

  15. Linking coupled motions and entropic effects to the catalytic activity of 2-deoxyribose-5-phosphate aldolase (DERA)

    Article • Chemical Science, January 2016, Royal Society of Chemistry

    Dr Shina Caroline Lynn Kamerlin, Professor Mikael Widersten, Shina Caroline Lynn Kamerlin

  16. Protein engineering for development of new hydrolytic biocatalysts

    Article • Current Opinion in Chemical Biology, August 2014, Elsevier

    Professor Mikael Widersten

  17. Substrate scope and selectivity in offspring to an enzyme subjected to directed evolution

    Article • European Journal of Biochemistry, April 2014, Wiley

    Professor Mikael Widersten

  18. Obtaining Optical Purity for Product Diols in Enzyme-Catalyzed Epoxide Hydrolysis: Contributions from Changes in both Enantio- and Regioselectivity

    Article • Biochemistry, September 2012, American Chemical Society (ACS)

    Professor Mikael Widersten

  19. One-step enzyme extraction and immobilization for biocatalysis applications

    Article • Biotechnology Journal, March 2011, Wiley

    Professor Mikael Widersten, Prof. Uwe T Bornscheuer

  20. Modification of Substrate Specificity Resulting in an Epoxide Hydrolase with Shifted Enantiopreference for (2,3-Epoxypropyl)benzene

    Article • ChemBioChem, June 2010, Wiley

    Professor Mikael Widersten

  21. Substrate-dependent hysteretic behavior in StEH1-catalyzed hydrolysis of styrene oxide derivatives

    Article • European Journal of Biochemistry, November 2008, Wiley

    Professor Mikael Widersten

  22. Removal of distal protein-water hydrogen bonds in a plant epoxide hydrolase increases catalytic turnover but decreases thermostability

    Article • Protein Science, July 2008, Wiley

    Professor Mikael Widersten

  23. Active Site of Epoxide Hydrolases Revisited:  A Noncanonical Residue in Potato StEH1 Promotes both Formation and Breakdown of the Alkylenzyme Intermediate †

    Article • Biochemistry, March 2007, American Chemical Society (ACS)

    Professor Mikael Widersten

  24. X-ray structure of potato epoxide hydrolase sheds light on substrate specificity in plant enzymes

    Article • Protein Science, July 2006, Wiley

    Professor Mikael Widersten

  25. Implications for an Ionized Alkyl-Enzyme Intermediate during StEH1-Catalyzed trans -Stilbene Oxide Hydrolysis †

    Article • Biochemistry, January 2006, American Chemical Society (ACS)

    Professor Mikael Widersten

  26. Catalysis of potato epoxide hydrolase, StEH1

    Article • Biochemical Journal, September 2005, Portland Press Ltd.

    Professor Mikael Widersten

  27. Repertoire Selection of Variant Single-Chain Cro:  Toward Directed DNA-Binding Specificity of Helix−Turn−Helix Proteins †

    Article • Biochemistry, September 2004, American Chemical Society (ACS)

    Professor Mikael Widersten

  28. Amino acid residue 247 in canine sulphotransferase SULT1D1: a new determinant of substrate selectivity

    Article • Biochemical Journal, March 2004, Portland Press Ltd.

    Professor Mikael Widersten

  29. Inhibition of glutathione S-transferases by antimalarial drugs possible implications for circumventing anticancer drug resistance

    Article • International Journal of Cancer, January 2002, Wiley

    Professor Mikael Widersten

  30. Preface

    Article • Chemico-Biological Interactions, February 2001, Elsevier

    Professor Mikael Widersten

  31. Proposed reductive metabolism of artemisinin by glutathione transferasesin vitro

    Article • Free Radical Research, January 2001, Taylor & Francis

    Professor Mikael Widersten

  32. Yeast Glyoxalase I Is a Monomeric Enzyme with Two Active Sites

    Article • Journal of Biological Chemistry, October 2000, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor Mikael Widersten

  33. Mechanism-Based Phage Display Selection of Active-Site Mutants of Human Glutathione Transferase A1-1 Catalyzing S N Ar Reactions †

    Article • Biochemistry, September 1997, American Chemical Society (ACS)

    Professor Mikael Widersten

  34. Optimized Heterologous Expression of the Polymorphic Human Glutathione Transferase M1-1 Based on Silent Mutations in the Corresponding cDNA

    Article • Protein Expression and Purification, June 1996, Elsevier

    Professor Mikael Widersten

  35. Glutathione Transferases with Novel Active Sites Isolated by Phage Display from a Library of Random Mutants

    Article • Journal of Molecular Biology, July 1995, Elsevier

    Professor Mikael Widersten

  36. Contribution of amino acid residue 208 in the hydrophobic binding site to the catalytic mechanism of human glutathione transferase A1-1

    Article • Biochemistry, October 1994, American Chemical Society (ACS)

    Professor Mikael Widersten

  37. GLUTATHIONE-LINKED ENZYMES IN DETOXICATION REACTIONS

    Article • January 1989, Elsevier

    Professor Mikael Widersten