All Stories

  1. Enantioselective Hydration of Non-CoA Enoyl-Thioesters by Enoyl-CoA Hydratase (ECH): Activation of the Active Site Oxyanion Hole with 3′,5′-Adenosine-Diphosphate Enables Competent Catalysis

    Article • JACS Au, March 2026, American Chemical Society (ACS)

    rik wierenga

  2. Targeting collagen biosynthesis by small molecules inhibiting the function of the peptide-substrate-binding domain of collagen prolyl 4-hydroxylases

    Article • February 2026, openRxiv

    rik wierenga

  3. Binding Differences of the Peptide‐Substrate–Binding Domain of Collagen Prolyl 4‐Hydroxylases I and II for Proline‐ and Hydroxyproline‐Rich Peptides

    Article • Proteins Structure Function and Bioinformatics, May 2025, Wiley

    rik wierenga

  4. Jan Drenth (1925–2025)

    Article • Acta Crystallographica Section D Structural Biology, April 2025, International Union of Crystallography

    Bauke Dijkstra, rik wierenga

  5. Crystallographic fragment-binding studies of the Mycobacterium tuberculosis trifunctional enzyme suggest binding pockets for the tails of the acyl-CoA substrates at its active sites and a potential substrate-channeling path between them

    Article • Acta Crystallographica Section D Structural Biology, July 2024, International Union of Crystallography

    rik wierenga

  6. Managing macromolecular crystallographic data with a laboratory information management system

    Article • Acta Crystallographica Section D Structural Biology, July 2024, International Union of Crystallography

    rik wierenga, Edward Daniel

  7. Structural enzymology studies with the substrate 3S‐hydroxybutanoyl‐CoA: bifunctional MFE1 is a less efficient dehydrogenase than monofunctional HAD

    Article • FEBS Open Bio, March 2024, Wiley

    rik wierenga, Professor Mikael Widersten

  8. Crystallographic fragment binding studies of theMycobacterium tuberculosistrifunctional enzyme suggest binding pockets for the tails of the acyl-CoA substrates at its active sites and a potential substrate channeling path between them

    Article • January 2024, Cold Spring Harbor Laboratory Press

    rik wierenga

  9. Enzymes of the crotonase superfamily: Diverse assembly and diverse function

    Article • Current Opinion in Structural Biology, October 2023, Elsevier

    rik wierenga

  10. Crystal structures and kinetic studies of a laboratory evolved aldehyde reductase explain the dramatic shift of its new substrate specificity

    Article • IUCrJ, June 2023, International Union of Crystallography

    rik wierenga, Professor Mikael Widersten

  11. An engineered variant of MECR reductase reveals indispensability of long-chain acyl-ACPs for mitochondrial respiration

    Article • Nature Communications, February 2023, Springer Science + Business Media

    rik wierenga

  12. Neutron structures of Leishmania mexicana triosephosphate isomerase in complex with reaction-intermediate mimics shed light on the proton-shuttling steps

    Article • IUCrJ, June 2021, International Union of Crystallography

    Dr Matthew P Blakeley, rik wierenga

  13. Structure of transmembrane prolyl 4-hydroxylase reveals unique organization of EF and dioxygenase domains

    Article • Journal of Biological Chemistry, January 2021, American Society for Biochemistry & Molecular Biology (ASBMB)

    rik wierenga

  14. Crystallographic binding studies of rat peroxisomal multifunctional enzyme type 1 with 3-ketodecanoyl-CoA: capturing active and inactive states of its hydratase and dehydrogenase catalytic sites

    Article • Acta Crystallographica Section D Structural Biology, November 2020, International Union of Crystallography

    rik wierenga

  15. Structure of transmembrane prolyl 4-hydroxylase reveals unique organization of EF and dioxygenase domains

    Article • October 2020, Cold Spring Harbor Laboratory Press

    rik wierenga

  16. A community proposal to integrate structural bioinformatics activities in ELIXIR (3D-Bioinfo Community)

    Article • F1000Research, April 2020, Faculty of 1000, Ltd.

    Dr. Alexandre M.J.J. Bonvin, Dr Anastassis Perrakis, rik wierenga, Prof. Bohdan Schneider, Dr John M Hancock

  17. Complementary substrate specificity and distinct quaternary assembly of the Escherichia coli aerobic and anaerobic β-oxidation trifunctional enzyme complexes

    Article • Biochemical Journal, July 2019, Portland Press Ltd.

    rik wierenga

  18. The peroxisomal zebrafish SCP2-thiolase (type-1) is a weak transient dimer as revealed by crystal structures and native mass spectrometry

    Article • Biochemical Journal, January 2019, Portland Press Ltd.

    rik wierenga

  19. Structural enzymology binding studies of the peptide-substrate-binding domain of human collagen prolyl 4-hydroxylase (type-II): High affinity peptides have a PxGP sequence motif

    Article • Protein Science, September 2018, Wiley

    rik wierenga

  20. Structural enzymology comparisons of multifunctional enzyme, type-1 (MFE1): the flexibility of its dehydrogenase part

    Article • FEBS Open Bio, November 2017, Wiley

    rik wierenga

  21. Crystal structures of two monomeric triosephosphate isomerase variants identifiedviaa directed-evolution protocol selecting forL-arabinose isomerase activity

    Article • Acta Crystallographica Section F Structural Biology Communications, May 2016, International Union of Crystallography

    rik wierenga

  22. The SCP2-thiolase-like protein (SLP) of Trypanosoma brucei is an enzyme involved in lipid metabolism

    Article • Proteins Structure Function and Bioinformatics, May 2016, Wiley

    rik wierenga

  23. Crystal structure of Trypanosoma brucei SCP2-thiolase like protein ( TbSLP) in complex with acetoacetyl-CoA.

    Article • May 2016, Protein Data Bank, Rutgers University

    rik wierenga

  24. Crystal structure of Trypanosoma brucei SCP2-thiolase like protein ( TbSLP) form-I.

    Article • May 2016, Protein Data Bank, Rutgers University

    rik wierenga

  25. Crystal structure of Trypanosoma brucei SCP2-thiolase like protein ( TbSLP) form-II.

    Article • May 2016, Protein Data Bank, Rutgers University

    rik wierenga

  26. The extended structure of the periplasmic region of CdsD, a structural protein of the type III secretion system of Chlamydia trachomatis

    Article • Protein Science, March 2016, Wiley

    rik wierenga

  27. Structures of yeast peroxisomal Δ3,Δ2-enoyl-CoA isomerase complexed with acyl-CoA substrate analogues: the importance of hydrogen-bond networks for the reactivity of the catalytic base and the oxyanion hole

    Article • Acta Crystallographica Section D Biological Crystallography, October 2015, International Union of Crystallography

    rik wierenga

  28. Human Δ3,Δ2-enoyl-CoA isomerase, type 2: a structural enzymology study on the catalytic role of its ACBP domain and helix-10

    Article • FEBS Journal, January 2015, Wiley

    rik wierenga

  29. The crystal structure of human mitochondrial 3-ketoacyl-CoA thiolase (T1): insight into the reaction mechanism of its thiolase and thioesterase activities

    Article • Acta Crystallographica Section D Biological Crystallography, November 2014, International Union of Crystallography

    rik wierenga

  30. The crystal structure of Δ3-Δ2-enoyl-CoA isomerase

    Article • Journal of Molecular Biology, June 2001, Elsevier

    rik wierenga

  31. Oxyanion Holes and Their Mimics

    Article • Wiley

    rik wierenga