All Stories

  1. BioProcessNexus: An open-source platform for surrogate techno economic models

    Article • Computers & Chemical Engineering, October 2025, Elsevier

    Gerald Striedner

  2. Scar-free tag removal by CASPON® enzyme with broad physicochemical stability in biomanufacturing – A case study of five proteins

    Article • Separation and Purification Technology, July 2025, Elsevier

    Gerald Striedner

  3. Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts: Quo Vadis?

    Article • Chemical Reviews, May 2025, American Chemical Society (ACS)

    Gerald Striedner

  4. Site-Directed Genome Integration via Recombinase-Mediated Cassette Exchange (RMCE) in Escherichia coli

    Article • ACS Synthetic Biology, April 2025, American Chemical Society (ACS)

    Gerald Striedner

  5. Aligning fermentation conditions with non-canonical amino acid addition strategy is essential for Nε-((2-azidoethoxy)carbonyl)-L-lysine uptake and incorporation into the target protein

    Article • Scientific Reports, October 2024, Springer Science + Business Media

    Gerald Striedner

  6. Manufacturing of the highly active thermophile PETases PHL7 and PHL7mut3 using Escherichia coli

    Article • Microbial Cell Factories, October 2024, Springer Science + Business Media

    Gerald Striedner

  7. Purification of recombinantly produced somatostatin-28 comparing hydrochloric acid and polyethyleneimine as E. coli extraction aids

    Article • Protein Expression and Purification, October 2024, Elsevier

    Gerald Striedner

  8. A production platform for disulfide-bonded peptides in the periplasm of Escherichia coli

    Article • Microbial Cell Factories, June 2024, Springer Science + Business Media

    Gerald Striedner

  9. Modifications of the 5’ region of the CASPONTM tag’s mRNA further enhance soluble recombinant protein production in Escherichia coli

    Article • Microbial Cell Factories, March 2024, Springer Science + Business Media

    Gerald Striedner

  10. Understanding the mechanism of polyethyleneimine-mediated cell disintegration and protein extraction in E. coli: The role of floc network formation and PEI molecular weight

    Article • Journal of Biotechnology, March 2024, Elsevier

    Gerald Striedner

  11. RNA-seq reveals multifaceted gene expression response to Fab production in Escherichia coli fed-batch processes with particular focus on ribosome stalling

    Article • Microbial Cell Factories, January 2024, Springer Science + Business Media

    Gerald Striedner

  12. Antibody fragments functionalized with non-canonical amino acids preserving structure and functionality - A door opener for new biological and therapeutic applications

    Article • Heliyon, December 2023, Elsevier

    Gerald Striedner

  13. Polyethyleneimine efficiently extracts recombinant cytoplasmatic green fluorescent protein produced in Escherichia coli with high purity

    Article • Journal of Biotechnology, July 2023, Elsevier

    Gerald Striedner

  14. Clickable Shiga Toxin B Subunit for Drug Delivery in Cancer Therapy

    Article • ACS Omega, April 2023, American Chemical Society (ACS)

    Gerald Striedner

  15. Scale‐related process heterogeneities change properties of high‐cell‐density fermentation broths demonstrated with Escherichia coli B and K‐12 strains

    Article • Journal of Chemical Technology & Biotechnology, March 2023, Wiley

    Gerald Striedner

  16. Recombinant Peptide Production Softens Escherichia coli Cells and Increases Their Size during C-Limited Fed-Batch Cultivation

    Article • International Journal of Molecular Sciences, January 2023, MDPI AG

    Gerald Striedner

  17. Development and Validation of an Artificial Neural-Network-Based Optical Density Soft Sensor for a High-Throughput Fermentation System

    Article • Processes, January 2023, MDPI AG

    Gerald Striedner

  18. Computational fluid dynamics simulation improves the design and characterization of a plug‐flow‐type scale‐down reactor for microbial cultivation processes

    Article • Biotechnology Journal, November 2022, Wiley

    Gerald Striedner

  19. CASPON platform technology: Ultrafast circularly permuted caspase-2 cleaves tagged fusion proteins before all 20 natural amino acids at the N-terminus

    Article • New Biotechnology, November 2022, Elsevier

    Gerald Striedner

  20. CRISPRactivation-SMS, a message for PAM sequence independent gene up-regulation in Escherichia coli

    Article • Nucleic Acids Research, September 2022, Oxford University Press (OUP)

    Gerald Striedner

  21. A general deep hybrid model for bioreactor systems: Combining first principles with deep neural networks

    Article • Computers & Chemical Engineering, September 2022, Elsevier

    Gerald Striedner

  22. Strain specific properties of Escherichia coli can prevent non-canonical amino acid misincorporation caused by scale-related process heterogeneities

    Article • Microbial Cell Factories, August 2022, Springer Science + Business Media

    Gerald Striedner

  23. Model predictive control for steady-state performance in integrated continuous bioprocesses

    Article • Bioprocess and Biosystems Engineering, August 2022, Springer Science + Business Media

    Gerald Striedner

  24. Fundamental insights in early‐stage inclusion body formation

    Article • Microbial Biotechnology, July 2022, Wiley

    Gerald Striedner

  25. Fusion Tag Design Influences Soluble Recombinant Protein Production in Escherichia coli

    Article • International Journal of Molecular Sciences, July 2022, MDPI AG

    Gerald Striedner

  26. Strain specific properties of Escherichia coli can prevent non-canonical amino acid misincorporation caused by scale-related process heterogeneities

    Article • June 2022, Research Square

    Gerald Striedner

  27. In-Depth Characterization of a Re-Engineered Cholera Toxin Manufacturing Process Using Growth-Decoupled Production in Escherichia coli

    Article • Toxins, June 2022, MDPI AG

    Gerald Striedner

  28. Bioprocess characterization of virus‐like particle production with the insect cell baculovirus expression system at nanoparticle level

    Article • Journal of Chemical Technology & Biotechnology, May 2022, Wiley

    Gerald Striedner

  29. Interaction of Periplasmic Fab Production and Intracellular Redox Balance in Escherichia coli Affects Product Yield

    Article • ACS Synthetic Biology, January 2022, American Chemical Society (ACS)

    Gerald Striedner

  30. Model Transferability and Reduced Experimental Burden in Cell Culture Process Development Facilitated by Hybrid Modeling and Intensified Design of Experiments

    Article • Frontiers in Bioengineering and Biotechnology, December 2021, Frontiers

    Gerald Striedner

  31. Production of full-length SARS-CoV-2 nucleocapsid protein from Escherichia coli optimized by native hydrophobic interaction chromatography hyphenated to multi-angle light scattering detection

    Article • Talanta, December 2021, Elsevier

    Gerald Striedner

  32. Proton-transfer-reaction mass spectrometry (PTR-MS) for online monitoring of glucose depletion and cell concentrations in HEK 293 gene therapy processes

    Article • Biotechnology Letters, November 2021, Springer Science + Business Media

    Gerald Striedner

  33. PROFICS: A bacterial selection system for directed evolution of proteases

    Article • Journal of Biological Chemistry, October 2021, Elsevier

    Gerald Striedner

  34. Stable Sf9 cell pools as a system for rapid HIV‐1 virus‐like particle production

    Article • Journal of Chemical Technology & Biotechnology, September 2021, Wiley

    Gerald Striedner

  35. Digital Twin Application for Model-Based DoE to Rapidly Identify Ideal Process Conditions for Space-Time Yield Optimization

    Article • Processes, June 2021, MDPI AG

    Gerald Striedner

  36. High shear resistance of insect cells: the basis for substantial improvements in cell culture process design

    Article • Scientific Reports, May 2021, Springer Science + Business Media

    Gerald Striedner

  37. A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests

    Article • EBioMedicine, May 2021, Elsevier

    Gerald Striedner

  38. Whole cell-based catalyst for enzymatic production of the osmolyte 2-O-α-glucosylglycerol

    Article • Microbial Cell Factories, April 2021, Springer Science + Business Media

    Gerald Striedner

  39. Native Hydrophobic Interaction Chromatography Hyphenated to Multi-Angle Light Scattering Detection for In-Process Control of SARS-CoV-2 Nucleocapsid Protein Produced in Escherichia Coli

    Article • March 2021, American Chemical Society (ACS)

    Gerald Striedner

  40. Holistic view on processes is essential for efficient production process development

    Article • Biotechnology Journal, March 2021, Wiley

    Mathias Fink, Gerald Striedner

  41. Tunable expression rate control of a growth-decoupled T7 expression system by l-arabinose only

    Article • Microbial Cell Factories, February 2021, Springer Science + Business Media

    Gerald Striedner

  42. Three-dimensional chromatography for purification and characterization of antibody fragments and related impurities from Escherichia coli crude extracts

    Article • Journal of Chromatography A, February 2021, Elsevier

    Gerald Striedner

  43. Advanced purification platform using circularly permuted caspase‐2 for affinity fusion‐tag removal to produce native fibroblast growth factor 2

    Article • Journal of Chemical Technology & Biotechnology, January 2021, Wiley

    Gerald Striedner

  44. Quantification of glycated IgG in CHO supernatants: A practical approach

    Article • Biotechnology Progress, January 2021, Wiley

    Gerald Striedner

  45. High-throughput microbioreactor provides a capable tool for early stage bioprocess development

    Article • Scientific Reports, January 2021, Springer Science + Business Media

    Gerald Striedner

  46. A comprehensive antigen production and characterization study for easy-to-implement, highly specific and quantitative SARS-CoV-2 antibody assays

    Article • January 2021, Cold Spring Harbor Laboratory Press

    Gerald Striedner

  47. Accelerating HIV‐1 VLP production using stable High Five insect cell pools

    Article • Biotechnology Journal, December 2020, Wiley

    Gerald Striedner

  48. Reconsider the shear paradigm - stirring and aeration strategies in cell culture processes

    Article • December 2020, Research Square

    Gerald Striedner

  49. Production of Circularly Permuted Caspase-2 for Affinity Fusion-Tag Removal: Cloning, Expression in Escherichia coli, Purification, and Characterization

    Article • Biomolecules, November 2020, MDPI AG

    Gerald Striedner

  50. Adaptive Evolution in Producing Microtiter Cultivations Generates Genetically Stable Escherichia coli Production Hosts for Continuous Bioprocessing

    Article • Biotechnology Journal, November 2020, Wiley

    Gerald Striedner

  51. Fast and antibiotic free genome integration into Escherichia coli chromosome

    Article • Scientific Reports, October 2020, Springer Science + Business Media

    Gerald Striedner

  52. PEI-Mediated Transient Transfection of High Five Cells at Bioreactor Scale for HIV-1 VLP Production

    Article • Nanomaterials, August 2020, MDPI AG

    Gerald Striedner

  53. Hybrid Modeling and Intensified DoE: An Approach to Accelerate Upstream Process Characterization

    Article • Biotechnology Journal, June 2020, Wiley

    Gerald Striedner

  54. Bacteriophage Inspired Growth-Decoupled Recombinant Protein Production in Escherichia coli

    Article • ACS Synthetic Biology, April 2020, American Chemical Society (ACS)

    Gerald Striedner

  55. Extraction of recombinant periplasmic proteins under industrially relevant process conditions: Selectivity and yield strongly depend on protein titer and methodology

    Article • Biotechnology Progress, April 2020, Wiley

    Gerald Striedner

  56. Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systems

    Article • Microbial Cell Factories, March 2020, Springer Science + Business Media

    Gerald Striedner

  57. Comparison of Modeling Methods for DoE‐Based Holistic Upstream Process Characterization

    Article • Biotechnology Journal, February 2020, Wiley

    Gerald Striedner

  58. Evaluation of screening platforms for virus-like particle production with the baculovirus expression vector system in insect cells

    Article • Scientific Reports, January 2020, Springer Science + Business Media

    Gerald Striedner

  59. Soft sensor based on 2D‐fluorescence and process data enabling real‐time estimation of biomass in Escherichia coli cultivations

    Article • Engineering in Life Sciences, November 2019, Wiley

    Gerald Striedner

  60. The shortcomings of accurate rate estimations in cultivation processes and a solution for precise and robust process modeling

    Article • Bioprocess and Biosystems Engineering, September 2019, Springer Science + Business Media

    Gerald Striedner

  61. Oxygen Uptake Rate Soft-Sensing via Dynamic kLa Computation: Cell Volume and Metabolic Transition Prediction in Mammalian Bioprocesses

    Article • Frontiers in Bioengineering and Biotechnology, August 2019, Frontiers

    Gerald Striedner

  62. Impact of mammalian cell culture conditions on monoclonal antibody charge heterogeneity: an accessory monitoring tool for process development

    Article • Journal of Industrial Microbiology & Biotechnology, August 2019, Oxford University Press (OUP)

    Gerald Striedner

  63. Microbioreactor Cultivations of Fab‐Producing Escherichia coli Reveal Genome‐Integrated Systems as Suitable for Prospective Studies on Direct Fab Expression Effects

    Article • Biotechnology Journal, July 2019, Wiley

    Mathias Fink, Gerald Striedner

  64. Implementation of a Fully Automated Microbial Cultivation Platform for Strain and Process Screening

    Article • Biotechnology Journal, June 2019, Wiley

    Gerald Striedner

  65. Lectin bio‐layer interferometry for assessing product quality of Fc‐ glycosylated immunoglobulin G

    Article • Biotechnology Progress, June 2019, Wiley

    Gerald Striedner

  66. Application of the Bradford Assay for Cell Lysis Quantification: Residual Protein Content in Cell Culture Supernatants

    Article • Biotechnology Journal, May 2019, Wiley

    Gerald Striedner

  67. Quality by control: Towards model predictive control of mammalian cell culture bioprocesses

    Article • Biotechnology Journal, March 2017, Wiley

    Wolfgang Sommeregger, Bernhard Sissolak, Gerald Striedner

  68. High-level biosynthesis of norleucine in E. coli for the economic labeling of proteins

    Article • Journal of Biotechnology, October 2016, Elsevier

    Gerald Striedner

  69. Boosted structured additive regression forEscherichia colifed-batch fermentation modeling

    Article • Biotechnology and Bioengineering, August 2016, Wiley

    Gerald Striedner

  70. Process limitations of a whole-cell P450 catalyzed reaction using a CYP153A-CPR fusion construct expressed in Escherichia coli

    Article • Applied Microbiology and Biotechnology, October 2015, Springer Science + Business Media

    Gerald Striedner

  71. Fed-batch like cultivation in a micro-bioreactor: screening conditions relevant for Escherichia coli based production processes

    Article • SpringerPlus, September 2015, Springer Science + Business Media

    Gerald Striedner

  72. The potential of random forest and neural networks for biomass and recombinant protein modeling inEscherichia colifed-batch fermentations

    Article • Biotechnology Journal, August 2015, Wiley

    Gerald Striedner

  73. Finished Genome Sequence of the Laboratory Strain Escherichia coli K-12 RV308 (ATCC 31608)

    Article • Genome Announcements, November 2014, ASM Journals

    Gerald Striedner

  74. Finished Genome Sequence of Escherichia coli K-12 Strain HMS174 (ATCC 47011)

    Article • Genome Announcements, November 2014, ASM Journals

    Gerald Striedner

  75. Online prediction of product titer and solubility of recombinant proteins in Escherichia colifed-batch cultivations

    Article • Journal of Chemical Technology & Biotechnology, July 2014, Wiley

    Gerald Striedner

  76. Preventing T7 RNA Polymerase Read-through Transcription—A Synthetic Termination Signal Capable of Improving Bioprocess Stability

    Article • ACS Synthetic Biology, May 2014, American Chemical Society (ACS)

    Gerald Striedner

  77. A Comparative Analysis of Industrial Escherichia coli K–12 and B Strains in High-Glucose Batch Cultivations on Process-, Transcriptome- and Proteome Level

    Article • PLOS One, August 2013, PLOS

    Gerald Striedner

  78. Evaluation of three industrial Escherichia coli strains in fed-batch cultivations during high-level SOD protein production

    Article • Microbial Cell Factories, June 2013, Springer Science + Business Media

    Gerald Striedner

  79. Comparative Transcription Profiling and In-Depth Characterization of Plasmid-Based and Plasmid-Free Escherichia coli Expression Systems under Production Conditions

    Article • Applied and Environmental Microbiology, April 2013, ASM Journals

    Gerald Striedner

  80. A sequence comparison and gene expression data integration add-on for the Pathway Tools software

    Article • Bioinformatics, July 2012, Oxford University Press (OUP)

    Gerald Striedner

  81. Implementation of proton transfer reaction-mass spectrometry (PTR-MS) for advanced bioprocess monitoring

    Article • Biotechnology and Bioengineering, July 2012, Wiley

    Gerald Striedner

  82. Soft sensors in bioprocessing: A status report and recommendations

    Article • Biotechnology Journal, April 2012, Wiley

    Professor Krist V. Gernaey, Gerald Striedner

  83. An Advanced Monitoring Platform for Rational Design of Recombinant Processes

    Article • January 2012, Springer Science + Business Media

    Gerald Striedner

  84. Process analytical technology (PAT) for biopharmaceuticals

    Article • Biotechnology Journal, March 2011, Wiley

    Professor Krist V. Gernaey, Gerald Striedner

  85. Marker-free plasmids for gene therapeutic applications—Lack of antibiotic resistance gene substantially improves the manufacturing process

    Article • Journal of Biotechnology, April 2010, Elsevier

    Gerald Striedner

  86. Plasmid-free T7-basedEscherichia coliexpression systems

    Article • Biotechnology and Bioengineering, January 2010, Wiley

    Gerald Striedner

  87. Interactive visualization of clusters in microarray data: an efficient tool for improved metabolic analysis of E. coli

    Article • Microbial Cell Factories, July 2009, Springer Science + Business Media

    Gerald Striedner

  88. Model-based probe set optimization for high-performance microarrays

    Article • Nucleic Acids Research, December 2008, Oxford University Press (OUP)

    Gerald Striedner

  89. Influence of process temperature on recombinant enzyme activity in Escherichia coli fed-batch cultures

    Article • Enzyme and Microbial Technology, December 2008, Elsevier

    Gerald Striedner

  90. Development of an antibiotic-free plasmid selection system based on glycine auxotrophy for recombinant protein overproduction in Escherichia coli

    Article • Journal of Biotechnology, March 2008, Elsevier

    Gerald Striedner

  91. Npro fusion technology to produce proteins with authentic N termini in E. coli

    Article • Nature Chemical Biology, November 2007, Springer Science + Business Media

    Gerald Striedner

  92. Using ColE1-derived RNA I for suppression of a bacterially encoded gene: implication for a novel plasmid addiction system

    Article • Biotechnology Journal, June 2006, Wiley

    Gerald Striedner

  93. Evaluation of the GFP signal and its aptitude for novel on-line monitoring strategies of recombinant fermentation processes

    Article • Journal of Biotechnology, March 2004, Elsevier

    Gerald Striedner

  94. Tuning the Transcription Rate of Recombinant Protein in Strong Escherichia coli Expression Systems through Repressor Titration

    Article • Biotechnology Progress, October 2003, Wiley

    Gerald Striedner

  95. Stabilizing plasmid copy number to improve recombinant protein production

    Article • Biotechnology and Bioengineering, December 2001, Wiley

    Gerald Striedner

  96. Non-invasive detection of the metabolic burden on recombinant microorganisms during fermentation processes

    Article • Journal of Chemical Technology & Biotechnology, July 2001, Wiley

    Gerald Striedner

  97. Recombinant Expression of Alliin Lyase from Garlic (Allium sativum) in Bacteria and Yeasts

    Article • Planta Medica, May 1998, Thieme Publishing Group

    Gerald Striedner