All Stories

  1. Illuminating High-Affinity ATP Binding to the Sodium-Potassium Pump Using Solid-State NMR Spectroscopy

    Article • Molecules, September 2025, MDPI AG

    Professor David Middleton

  2. Complete removal of toxic herbicides and structural insight by a heteromolecular aggregate

    Article • Journal of Hazardous Materials, September 2025, Elsevier

    Professor David Middleton

  3. Conformational Analysis of Uniformly 13C-Labeled Peptides by Rotationally Selected 13Cα-13CH3 Double-Quantum Solid-State NMR

    Article • Molecules, February 2025, MDPI AG

    Professor David Middleton

  4. Encapsulation of toxic liquid molecules and adsorption of water pollutants by a versatile pre-organized single crystalline coating material

    Article • Inorganic Chemistry Frontiers, January 2025, Royal Society of Chemistry

    Professor David Middleton

  5. NMR studies of amyloid interactions

    Article • Progress in Nuclear Magnetic Resonance Spectroscopy, November 2024, Elsevier

    Professor David Middleton

  6. Characterization of Olive Oil Phenolic Extracts and Their Effects on the Aggregation of the Alzheimer’s Amyloid-β Peptide and Tau

    Article • ACS Omega, July 2024, American Chemical Society (ACS)

    Professor David Middleton

  7. Solid-state NMR chemical shift analysis for determining the conformation of ATP bound to Na,K-ATPase in its native membrane

    Article • RSC Advances, January 2023, Royal Society of Chemistry

    Professor David Middleton

  8. Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization

    Article • Aging Cell, November 2022, Wiley

    Manuel Mayr, Professor David Middleton

  9. Analysis of the orientation of cholesterol in high-density lipoprotein nanodiscs using solid-state NMR

    Article • Physical Chemistry Chemical Physics, January 2022, Royal Society of Chemistry

    Professor David Middleton

  10. Aggregation Kinetics and Filament Structure of a Tau Fragment Are Influenced by the Sulfation Pattern of the Cofactor Heparin

    Article • Biochemistry, September 2020, American Chemical Society (ACS)

    Professor David Middleton

  11. Raman Spectroscopy with 2D Perturbation Correlation Moving Windows for the Characterization of Heparin–Amyloid Interactions

    Article • Analytical Chemistry, September 2020, American Chemical Society (ACS)

    Professor David Middleton

  12. Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using 31P NMR Spectroscopy

    Article • Angewandte Chemie, August 2020, Wiley

    Professor David Middleton

  13. Circular Dichroism Spectroscopy Identifies the β-Adrenoceptor Agonist Salbutamol As a Direct Inhibitor of Tau Filament Formation in Vitro

    Article • ACS Chemical Neuroscience, June 2020, American Chemical Society (ACS)

    Professor David Middleton

  14. Drug orientations within statin-loaded lipoprotein nanoparticles by 19F solid-state NMR

    Article • Chemical Communications, January 2019, Royal Society of Chemistry

    Professor David Middleton

  15. Orientation of a Diagnostic Ligand Bound to Macroscopically Aligned Amyloid-β Fibrils Determined by Solid-State NMR

    Article • The Journal of Physical Chemistry Letters, October 2018, American Chemical Society (ACS)

    Professor David Middleton

  16. Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin

    Article • Journal of Biological Chemistry, May 2018, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor David Middleton

  17. Average orientation of a fluoroaromatic molecule in lipid bilayers from DFT-informed NMR measurements of 1H–19F dipolar couplings

    Article • Physical Chemistry Chemical Physics, January 2018, Royal Society of Chemistry

    Professor David Middleton

  18. Molecular Origins of the Compatibility between Glycosaminoglycans and Aβ40 Amyloid Fibrils

    Article • Journal of Molecular Biology, August 2017, Elsevier

    Professor David Middleton

  19. Heparin and Methionine Oxidation Promote the Formation of Apolipoprotein A-I Amyloid Comprising α-Helical and β-Sheet Structures

    Article • Biochemistry, March 2017, American Chemical Society (ACS)

    Professor David Middleton

  20. Atomic Details of the Interactions of Glycosaminoglycans with Amyloid-β Fibrils

    Article • Journal of the American Chemical Society, July 2016, American Chemical Society (ACS)

    Professor David Middleton

  21. Novel stereoselective bufadienolides reveal new insights into the requirements for Na+, K+-ATPase inhibition by cardiotonic steroids

    Article • Scientific Reports, July 2016, Springer Science + Business Media

    Professor David Middleton

  22. Long-Range Effects of Na+ Binding in Na,K-ATPase Reported by ATP

    Article • Biochemistry, November 2015, American Chemical Society (ACS)

    Professor David Middleton

  23. Comparisons with Amyloid-β Reveal an Aspartate Residue That Stabilizes Fibrils of the Aortic Amyloid Peptide Medin

    Article • Journal of Biological Chemistry, January 2015, American Society for Biochemistry & Molecular Biology (ASBMB)

    Professor David Middleton

  24. Ligand orientation in a membrane-embedded receptor site revealed by solid-state NMR with paramagnetic relaxation enhancement

    Article • Organic & Biomolecular Chemistry, January 2015, Royal Society of Chemistry

    Professor David Middleton, Professor Simon G. Patching

  25. Comparison of the Structure and Function of Phospholamban and the Arginine-14 Deficient Mutant Associated with Dilated Cardiomyopathy

    Article • PLOS One, September 2014, PLOS

    Professor David Middleton

  26. Expression and purification of the aortic amyloid polypeptide medin

    Article • Protein Expression and Purification, June 2014, Elsevier

    Professor David Middleton

  27. Synthesis of bufalin derivatives with inhibitory activity against prostate cancer cells

    Article • Natural Product Research, January 2014, Taylor & Francis

    Professor David Middleton

  28. Structures, chemotaxonomic significance, cytotoxic and Na+,K+-ATPase inhibitory activities of new cardenolides from Asclepias curassavica

    Article • Organic & Biomolecular Chemistry, January 2014, Royal Society of Chemistry

    Professor Mikael Esmann, Professor David Middleton

  29. Heparin Promotes the Rapid Fibrillization of a Peptide with Low Intrinsic Amyloidogenicity

    Article • Biochemistry, December 2013, American Chemical Society (ACS)

    Professor David Middleton

  30. Insights into the Molecular Architecture of a Peptide Nanotube Using FTIR and Solid-State NMR Spectroscopic Measurements on an Aligned Sample

    Article • Angewandte Chemie, August 2013, Wiley

    Professor David Middleton

  31. Probing the contacts of a low-affinity substrate with a membrane-embedded transport protein using1H-13C cross-polarisation magic-angle spinning solid-state NMR

    Article • Molecular Membrane Biology, November 2012, Taylor & Francis

    Professor Simon G. Patching, Professor David Middleton

  32. Site-Specific Identification of an Aβ Fibril-Heparin Interaction Site by Using Solid-State NMR Spectroscopy

    Article • Angewandte Chemie, November 2012, Wiley

    Professor Alison Rodger, Professor David Middleton

  33. Amyloid-Derived Peptide Forms Self-Assembled Monolayers on Gold Nanoparticle with a Curvature-Dependent β-Sheet Structure

    Article • ACS Nano, January 2012, American Chemical Society (ACS)

    Professor David Middleton

  34. Fibrils and nanotubes assembled from a modified amyloid-β peptide fragment differ in the packing of the same β-sheet building blocks

    Article • Chemical Communications, January 2012, Royal Society of Chemistry

    Professor David Middleton

  35. Solid-state NMR reveals differences in the packing arrangements of peptide aggregates derived from the aortic amyloid polypeptide medin

    Article • Journal of Peptide Science, November 2011, Wiley

    Professor David Middleton

  36. The Conformation of ATP within the Na,K-ATPase Nucleotide Site: A Statistically Constrained Analysis of REDOR Solid-State NMR Data

    Article • Angewandte Chemie, June 2011, Wiley

    Professor David Middleton

  37. A study of the membrane association and regulatory effect of the phospholemman cytoplasmic domain

    Article • Biochimica et Biophysica Acta (BBA) - Biomembranes, April 2011, Elsevier

    Professor David Middleton

  38. Solid-state NMR detection of 14N13C dipolar couplings between amino acid side groups provides constraints on amyloid fibril architecture

    Article • Magnetic Resonance in Chemistry, January 2011, Wiley

    Professor David Middleton

  39. Heparin-derived oligosaccharides interact with the phospholamban cytoplasmic domain and stimulate SERCA function

    Article • Biochemical and Biophysical Research Communications, October 2010, Elsevier

    Professor David Middleton

  40. Comparison of aggregation enhancement and inhibition as strategies for reducing the cytotoxicity of the aortic amyloid polypeptide medin

    Article • European Biophysics Journal, February 2010, Springer Science + Business Media

    Professor David Middleton

  41. Measurement of multiple torsional angles from one-dimensional solid-state NMR spectra: application to the conformational analysis of a ligand in its biological receptor site

    Article • Physical Chemistry Chemical Physics, January 2010, Royal Society of Chemistry

    Professor David Middleton

  42. Evaluation of β-Alanine- and GABA-Substituted Peptides as Inhibitors of Disease-Linked Protein Aggregation

    Article • ChemBioChem, August 2009, Wiley

    Professor David Middleton

  43. Inhibitors of protein aggregation and toxicity

    Article • Biochemical Society Transactions, August 2009, Portland Press Ltd.

    Prof. Andrew Doig, Professor David Middleton

  44. Structural analysis of uniformly 13C-labelled solids from selective angle measurements at rotational resonance

    Article • Journal of Magnetic Resonance, August 2009, Elsevier

    Professor Simon G. Patching, Professor David Middleton

  45. Solid-State NMR Studies of Adenosine 5′-Triphosphate Freeze-Trapped in the Nucleotide Site of Na,K-ATPase

    Article • ChemBioChem, June 2009, Wiley

    Professor David Middleton

  46. Cross-β Spine Architecture of Fibrils Formed by the Amyloidogenic Segment NFGSVQFV of Medin from Solid-State NMR and X-ray Fiber Diffraction Measurements†

    Article • Biochemistry, April 2009, American Chemical Society (ACS)

    Professor David Middleton

  47. Cytoplasmic residues of phospholamban interact with membrane surfaces in the presence of SERCA: A new role for phospholipids in the regulation of cardiac calcium cycling?

    Article • Biochimica et Biophysica Acta (BBA) - Biomembranes, February 2009, Elsevier

    Professor David Middleton

  48. Exploiting a 13C-labelled heparin analogue for in situ solid-state NMR investigations of peptide-glycan interactions within amyloid fibrils

    Article • Organic & Biomolecular Chemistry, January 2009, Royal Society of Chemistry

    Professor David Middleton

  49. Structural Insights into the Polymorphism of Amyloid-Like Fibrils Formed by Region 20−29 of Amylin Revealed by Solid-State NMR and X-ray Fiber Diffraction

    Article • Journal of the American Chemical Society, November 2008, American Chemical Society (ACS)

    Professor David Middleton

  50. Design of an N-Methylated Peptide Inhibitor of α-Synuclein Aggregation Guided by Solid-State NMR§

    Article • Journal of the American Chemical Society, June 2008, American Chemical Society (ACS)

    Prof. Andrew Doig, Professor David Middleton

  51. The dynamics and orientation of a lipophilic drug within model membranes determined by 13C solid-state NMR

    Article • Physical Chemistry Chemical Physics, January 2008, Royal Society of Chemistry

    Professor David Middleton

  52. Relative substrate affinities of wild-type and mutant forms of theEscherichia colisugar transporter GalP determined by solid-state NMR

    Article • Molecular Membrane Biology, January 2008, Taylor & Francis

    George Psakis, Professor Simon G. Patching, Professor David Middleton

  53. The effects of α-synuclein on phospholipid vesicle integrity: a study using31P NMR and electron microscopy

    Article • Molecular Membrane Biology, January 2008, Taylor & Francis

    Prof. Andrew Doig, Professor David Middleton

  54. Solid-State NMR Spectroscopy Detects Interactions between Tryptophan Residues of theE. coliSugar Transporter GalP and the α-Anomer of thed-Glucose Substrate

    Article • Journal of the American Chemical Society, January 2008, American Chemical Society (ACS)

    Professor Simon G. Patching, Professor David Middleton

  55. Membrane interactions of peptides representing the polybasic regions of three Rho GTPases are sensitive to the distribution of arginine and lysine residues

    Article • Molecular Membrane Biology, January 2008, Taylor & Francis

    Professor David Middleton