All Stories

  1. Polarising Agents and Spin Tags Used in DNP-ssNMR Studies on Biological Samples: An Update

    Article • April 2024, Sciencedomain International

    Professor Simon G. Patching

  2. Microscale Thermophoresis Analysis of Membrane Proteins

    Article • Chemical Science International Journal, March 2024, Sciencedomain International

    Professor Simon G. Patching

  3. Microscale Thermophoresis Analysis of Membrane Proteins

    Article • January 2024, Center for Open Science

    Professor Simon G. Patching

  4. Polarising Agents and Spin Tags for Dynamic Nuclear Polarisation (DNP)-Enhanced Solid-State Nuclear Magnetic Resonance (ssNMR) Analysis of Biological Samples

    Article • Current Journal of Applied Science and Technology, September 2023, Sciencedomain International

    Professor Simon G. Patching

  5. Elevator Mechanism of Alternating Access in theEscherichia coliConcentrative Nucleoside Transporter NupC

    Article • February 2023, Cold Spring Harbor Laboratory Press

    Professor Simon G. Patching

  6. In Vitro and In Silico Studies for the Identification of Potent Metabolites of Some High-Altitude Medicinal Plants from Nepal Inhibiting SARS-CoV-2 Spike Protein

    Article • Molecules, December 2022, MDPI AG

    Professor Simon G. Patching

  7. Article • Pakistan Journal of Pharmaceutical Sciences, November 2022, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi

    Professor Simon G. Patching

  8. Polyamine binding to a bacterial efflux pump involved in antimicrobial resistance

    Article • Radiation, May 2022, MDPI AG

    Professor Simon G. Patching

  9. Development of Antibiofilm Therapeutics Strategies to Overcome Antimicrobial Drug Resistance

    Article • Microorganisms, January 2022, MDPI AG

    Professor Simon G. Patching

  10. Spermidine Binding to the Acetinobacter baumannii Efflux Protein AceI Observed by Near-UV Synchrotron Radiation Circular Dichroism Spectroscopy

    Article • January 2022, MDPI AG

    Professor Simon G. Patching

  11. Cloning, Amplified Expression and Bioinformatics Analysis of a Putative Nucleobase Cation Symporter-1 (NCS-1) Protein From Rhodococcus erythropolis

    Article • BioScientific Review, November 2021, University of Management and Technology

    Professor Simon G. Patching

  12. Prevalence and molecular characterization of multidrug‐resistant Escherichia coli O157 : H7 from dairy milk in the Peshawar region of Pakistan

    Article • Journal of Food Safety, October 2021, Wiley

    Professor Simon G. Patching

  13. Green synthesis of gold nanoparticles using Tribulus terrestris extract and antibacterial activity against Gram-negative bacteria

    Article • Biomedical Letters, September 2021, The Science Publishers

    Professor Simon G. Patching

  14. Nutritional and lifestyle changes required for minimizing the recovery period in home quarantined COVID‐19 patients of Punjab, Pakistan

    Article • Food Science & Nutrition, July 2021, Wiley

    Professor Simon G. Patching

  15. Screening for hepatitis b and c viral infections among pregnant women attending the Bolan Medical Complex Hospital and Sandeman Provincial Civil Hospital Quetta, Pakistan.

    Article • The Professional Medical Journal, July 2020, Independent Medical Trust

    Professor Simon G. Patching

  16. In silico identification and structure function analysis of a putative coclaurine N-methyltransferase from Aristolochia fimbriata

    Article • Computational Biology and Chemistry, April 2020, Elsevier

    Professor Simon G. Patching

  17. Predominance of Central Asian strain (ST 26) in Mycobacterium tuberculosis isolates from Balochistan by spoligotyping

    Article • The Journal of Infection in Developing Countries, July 2019, Journal of Infection in Developing Countries

    Professor Simon G. Patching

  18. Prevalence of Salmonella spp. in chicken meat from Quetta retail outlets and typing through multiplex PCR

    Article • ROMANIAN BIOTECHNOLOGICAL LETTERS, April 2019, Digital ProScholar Media

    Professor Simon G. Patching

  19. Multiplex polymerase chain reaction detection of Shiga toxin genes and antibiotic sensitivity of Escherichia coli O157:H7 isolated from beef meat in Quetta, Pakistan

    Article • Journal of Food Safety, September 2018, Wiley

    Professor Simon G. Patching

  20. Recent developments in nucleobase cation symporter-1 (NCS1) family transport proteins from bacteria, archaea, fungi and plants

    Article • Journal of Biosciences, July 2018, Springer Science + Business Media

    Professor Simon G. Patching

  21. Pumping of drug molecules out of bacterial cells is a major mechanism of antibiotic resistance

    Article • Current Drug Targets, April 2018, Bentham Science Publishers

    Massoud Saidijam, Professor Simon G. Patching, Fatemeh Karimi Dermani

  22. Overcoming challenges for amplified expression of recombinant proteins using Escherichia coli

    Article • Protein Expression and Purification, April 2018, Elsevier

    Professor Simon G. Patching

  23. Nucleoside transporters in PET imaging of proliferating cancer cells using 3ꞌ-deoxy-3ꞌ-[18F]fluoro-L-thymidine

    Article • Journal of Diagnostic Imaging in Therapy, February 2018, Open Medscience Limited

    Professor Simon G. Patching

  24. Retraction of “Characterization of the Escherichia coli Concentrative Nucleoside Transporter NupC Using Computational, Biochemical, and Biophysical Methods”

    Article • Biochemistry, July 2017, American Chemical Society (ACS)

    Professor Simon G. Patching

  25. Efflux proteins at the blood–brain barrier: review and bioinformatics analysis

    Article • Xenobiotica, May 2017, Taylor & Francis

    Massoud Saidijam, Professor Simon G. Patching

  26. Hydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin

    Article • Scientific Reports, April 2017, Springer Science + Business Media

    Professor Simon G. Patching

  27. Synthesis, NMR analysis and applications of isotope-labelled hydantoins

    Article • Journal of Diagnostic Imaging in Therapy, February 2017, Open Medscience Limited

    Professor Simon G. Patching

  28. Comprehensive analysis of the numbers, lengths and amino acid compositions of transmembrane helices in prokaryotic, eukaryotic and viral integral membrane proteins of high-resolution structure

    Article • Journal of Biomolecular Structure and Dynamics, February 2017, Taylor & Francis

    Professor Simon G. Patching

  29. Characterisation of the DAACS Family Escherichia coli Glutamate/Aspartate-Proton Symporter GltP Using Computational, Chemical, Biochemical and Biophysical Methods

    Article • The Journal of Membrane Biology, December 2016, Springer Science + Business Media

    Professor Simon G. Patching

  30. Amino acid composition analysis of human secondary transport proteins and implications for reliable membrane topology prediction

    Article • Journal of Biomolecular Structure and Dynamics, July 2016, Taylor & Francis

    Professor Simon G. Patching

  31. NMR-Active Nuclei for Biological and Biomedical Applications

    Article • Journal of Diagnostic Imaging in Therapy, June 2016, Open Medscience Limited

    Professor Simon G. Patching

  32. Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions

    Article • Biochemical Society Transactions, June 2016, Portland Press Ltd.

    Professor Simon G. Patching

  33. Allantoin transport protein, PucI, from Bacillus subtilis: evolutionary relationships, amplified expression, activity and specificity

    Article • Microbiology, May 2016, Society for General Microbiology

    Professor Simon G. Patching

  34. Glucose Transporters at the Blood-Brain Barrier: Function, Regulation and Gateways for Drug Delivery

    Article • Molecular Neurobiology, January 2016, Springer Science + Business Media

    Professor Simon G. Patching

  35. Deuterated detergents for structural and functional studies of membrane proteins: Properties, chemical synthesis and applications

    Article • Molecular Membrane Biology, November 2015, Taylor & Francis

    Professor Simon G. Patching

  36. Solid-state NMR structures of integral membrane proteins

    Article • Molecular Membrane Biology, November 2015, Taylor & Francis

    Professor Simon G. Patching

  37. Roles of facilitative glucose transporter GLUT1 in [18F]FDG positron emission tomography (PET) imaging of human diseases

    Article • Journal of Diagnostic Imaging in Therapy, March 2015, Open Medscience Limited

    Professor Simon G. Patching

  38. Amino acid composition analysis of secondary transport proteins fromEscherichia coliwith relation to functional classification, ligand specificity and structure

    Article • Journal of Biomolecular Structure and Dynamics, January 2015, Taylor & Francis

    Professor Simon G. Patching

  39. Ligand orientation in a membrane-embedded receptor site revealed by solid-state NMR with paramagnetic relaxation enhancement

    Article • Organic & Biomolecular Chemistry, January 2015, Royal Society of Chemistry

    Professor David Middleton, Professor Simon G. Patching

  40. Synthesis of uniformly deuteratedn-dodecyl-β-d-maltoside (d39-DDM) for solubilization of membrane proteins in TROSY NMR experiments

    Article • Journal of Labelled Compounds and Radiopharmaceuticals, December 2014, Wiley

    Professor Simon G. Patching

  41. Molecular mechanism of ligand recognition by membrane transport protein, Mhp1

    Article • The EMBO Journal, June 2014, EMBO

    Professor Simon G. Patching

  42. TROSY NMR with a 52 kDa sugar transport protein and the binding of a small-molecule inhibitor

    Article • Molecular Membrane Biology, May 2014, Taylor & Francis

    Professor Simon G. Patching

  43. Ligand- and drug-binding studies of membrane proteins revealed through circular dichroism spectroscopy

    Article • Biochimica et Biophysica Acta (BBA) - Biomembranes, January 2014, Elsevier

    Professor Simon G. Patching

  44. Surface plasmon resonance spectroscopy for characterisation of membrane protein–ligand interactions and its potential for drug discovery

    Article • Biochimica et Biophysica Acta (BBA) - Biomembranes, January 2014, Elsevier

    Professor Simon G. Patching

  45. Transcriptomic and biochemical analyses identify a family of chlorhexidine efflux proteins

    Article • Proceedings of the National Academy of Sciences, November 2013, Proceedings of the National Academy of Sciences

    Professor Simon G. Patching

  46. Solid-State NMR Spectroscopy in Drug Design and Discovery

    Article • October 2013, Wiley

    Professor Simon G. Patching

  47. Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptideinhibitor siamycin I revealed through synchrotron radiation circular dichroism

    Article • Physical Chemistry Chemical Physics, January 2013, Royal Society of Chemistry

    Professor Simon G. Patching

  48. Probing the contacts of a low-affinity substrate with a membrane-embedded transport protein using1H-13C cross-polarisation magic-angle spinning solid-state NMR

    Article • Molecular Membrane Biology, November 2012, Taylor & Francis

    Professor Simon G. Patching, Professor David Middleton

  49. A systematic approach to the amplified expression, functional characterization and purification of inositol transporters fromBacillus subtilis

    Article • Molecular Membrane Biology, October 2012, Taylor & Francis

    Professor Simon G. Patching

  50. Interactions of the intact FsrC membrane histidine kinase with its pheromone ligand GBAP revealed through synchrotron radiation circular dichroism

    Article • Biochimica et Biophysica Acta (BBA) - Biomembranes, July 2012, Elsevier

    Professor Simon G. Patching

  51. NMR structures of polytopic integral membrane proteins

    Article • Molecular Membrane Biology, August 2011, Taylor & Francis

    Professor Simon G. Patching

  52. The Nucleobase-Cation-Symport-1 Family of Membrane Transport Proteins

    Article • December 2010, Wiley

    Professor Simon G. Patching

  53. Benzothioxalone derivatives as novel inhibitors of UDP-N-acetylglucosamine enolpyruvyl transferases (MurA and MurZ)

    Article • Journal of Antimicrobial Chemotherapy, September 2010, Oxford University Press (OUP)

    Professor Simon G. Patching

  54. Efficient syntheses of 13C- and 14C-labelled 5-benzyl and 5-indolylmethyl L-hydantoins

    Article • Journal of Labelled Compounds, January 2010, Wiley

    Professor Simon G. Patching

  55. Structural genomics of bacterial membrane transport proteins

    Article • Acta Crystallographica Section A Foundations of Crystallography, August 2009, International Union of Crystallography

    Professor Simon G. Patching

  56. Structural analysis of uniformly 13C-labelled solids from selective angle measurements at rotational resonance

    Article • Journal of Magnetic Resonance, August 2009, Elsevier

    Professor Simon G. Patching, Professor David Middleton

  57. Synthesis of highly pure14C-labelledDL-allantoin and13C NMR analysis of labelling integrity

    Article • Journal of Labelled Compounds, July 2009, Wiley

    Professor Simon G. Patching

  58. Structure and Molecular Mechanism of a Nucleobase-Cation-Symport-1 Family Transporter

    Article • Science, October 2008, American Association for the Advancement of Science

    Professor Simon G. Patching

  59. 2-Aminotetralones: Novel inhibitors of MurA and MurZ

    Article • Bioorganic & Medicinal Chemistry Letters, March 2008, Elsevier

    Professor Simon G. Patching

  60. Relative substrate affinities of wild-type and mutant forms of theEscherichia colisugar transporter GalP determined by solid-state NMR

    Article • Molecular Membrane Biology, January 2008, Taylor & Francis

    George Psakis, Professor Simon G. Patching, Professor David Middleton

  61. Probing metal ion substrate-binding to theE. coliZitB exporter in native membranes by solid state NMR

    Article • Molecular Membrane Biology, January 2008, Taylor & Francis

    Professor Simon G. Patching

  62. Solid-State NMR Spectroscopy Detects Interactions between Tryptophan Residues of theE. coliSugar Transporter GalP and the α-Anomer of thed-Glucose Substrate

    Article • Journal of the American Chemical Society, January 2008, American Chemical Society (ACS)

    Professor Simon G. Patching, Professor David Middleton

  63. Active membrane transport and receptor proteins from bacteria

    Article • Biochemical Society Transactions, August 2005, Portland Press Ltd.

    George Psakis, Professor Simon G. Patching

  64. The nucleoside transport proteins, NupC and NupG, from Escherichia coli: specific structural motifs necessary for the binding of ligands

    Article • Organic & Biomolecular Chemistry, January 2005, Royal Society of Chemistry

    Professor Simon G. Patching

  65. Substrate Affinities for Membrane Transport Proteins Determined by13C Cross-Polarization Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy

    Article • Journal of the American Chemical Society, March 2004, American Chemical Society (ACS)

    Professor Simon G. Patching

  66. Purification and properties of theEscherichia colinucleoside transporter NupG, a paradigm for a major facilitator transporter sub-family

    Article • Molecular Membrane Biology, January 2004, Taylor & Francis

    A/Prof Rietie Venter, Professor Simon G. Patching

  67. The economical synthesis of [2′-13C, 1,3-15N2]uridine; preliminary conformational studies by solid state NMR

    Article • Organic & Biomolecular Chemistry, January 2003, Royal Society of Chemistry

    Professor Simon G. Patching

  68. A Solid State NMR Approach to the Structure-Activity Relationship of the Nucleoside Transport Protein NupC ofEscherichia coli

    Article • Biochemical Society Transactions, June 2000, Portland Press Ltd.

    Professor Simon G. Patching

  69. Equipping a Research Scale Fermentation Laboratory for Production of Membrane Proteins

    Article • Wiley

    A/Prof Rietie Venter, Professor Simon G. Patching