What is it about?
Lactobacillus kefiranofaciens is an indicator microorganism for kefir and a key factor in kefir grain formation and kefiran production. We designed a novel real-time PCR primer and probe set, LKF_KU504, for the rapid detection of L. kefiranofaciens. In inclusivity and exclusivity tests, only 14 L. kefiranofaciens strains were positive among 61 microorganisms, indicating 100% sensitivity and specificity.
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Why is it important?
The LKF_KU504 set also differentiated kefir milk from 30 commercial nonkefir yogurts. The levels of L. kefiranofaciens in kefir grain and kefir milk were significantly different, indicating L. kefiranofaciens was more concentrated in kefir grain than in kefir milk.
Perspectives
we successfully utilized a real-time PCR assay to detect L. kefiranofaciens. This newly developed assay could be useful to the kefir industry because it could provide an accurate and rapid tool for detection and enumeration of L. kefiranofaciens in kefir grain and kefir milk for monitoring the quality of kefir probiotics.
Professor Kun-Ho Seo
Konkuk University
Read the Original
This page is a summary of: Rapid Detection of Lactobacillus kefiranofaciens in Kefir Grain and Kefir Milk Using Newly Developed Real-Time PCR, Journal of Food Protection, April 2015, International Association for Food Protection,
DOI: 10.4315/0362-028x.jfp-14-329.
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