What is it about?
This is a video protocol that details how to express and purify an engineered version of the N-terminal domain of NEMO, stabilized by coiled-coil adaptors. The protocol explains how to crystallize the protein in its unbound form and determine the structure.
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Why is it important?
Structure determination of the IKK-binding domain of NEMO is challenging: this protocol will enable the study of NEMO with small molecule inhibitors, to guide structure-based drug design.
Read the Original
This page is a summary of: Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO, Journal of Visualized Experiments, December 2019, MyJove Corporation,
DOI: 10.3791/60339.
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