What is it about?
Using a commercially available membrane, we can get enough cells for cell sorting, or even other applications such as PCR, western blot and immunofluorescence.
Featured Image
Photo by Richard Horvath on Unsplash
Why is it important?
With the advent of personalised medicine, it may be necessary to quantify levels of cell surface proteins such as LFA1, before starting patients on specific therapy.
Perspectives
The quantification of cell surface proteins is critical to classify dry eye patients and select appropriate patients for therapy.
Prof Louis Tong
National University of Singapore
Read the Original
This page is a summary of: A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva, Journal of Visualized Experiments, July 2017, MyJove Corporation,
DOI: 10.3791/55591.
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