What is it about?
This study focuses on exploring how the time interval between blood collection and the isolation of Peripheral Blood Mononuclear Cells (PBMCs) influences the gene activity in various immune cells, including T cells, NK cells, monocytes, and B cells. It employs single cell RNA sequencing technology to analyze these effects.
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Why is it important?
Since PBMCs are widely used in disease research, understanding how time and storage affect them is vital for accurate results. If gene activity changes due to these factors, it could lead to misinterpretation of data in disease studies. The study found that all major PBMC cell types showed changes in gene expression over time, especially in genes regulated by specific cellular pathways (like NF-kB in response to TNF, and AP-1 signaling). This provides valuable insights into how cells respond to environmental changes, such as being outside the body and stored.
Perspectives
This study adds to the broader understanding of how pre-analytical variables (like sample collection and processing) can impact biomedical research, emphasizing the need for careful experimental design. There’s a need for standardized timing and storage methods for blood samples in research to ensure consistency and accuracy in results.
Dr. Hui-Qi Qu
Children's Hospital of Philadelphia
Read the Original
This page is a summary of: Single Cell Transcriptome Analysis of Peripheral Blood Mononuclear Cells in Freshly Isolated versus Stored Blood Samples, Genes, January 2023, MDPI AG,
DOI: 10.3390/genes14010142.
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