What is it about?
We try to explain firstly how the concept of the initiator of DNA replication(DnaA) in E.coli is conceived and then how DnaA was isolated .The in vitro studies on DnaA and oriC brought about the explanation on how DnaA opens oriC,licensing of initiation. We try to reevaluate these in vitro studies compared to those in vivo which produced the concept of Initiation Mass. Introducing the nucleotypic effect,the possibility is dicussed about the Initiation mass as the consequence of initiation rather than a launching element.Another question of how desequestration of hemimethylated oriC is examined taken into account the cellular metabolisms.
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Why is it important?
Reevaluation of in vitro studies on inititiation of replication control taking into account in vivo stdies.
Perspectives
Reconsider the mechanisms of eclipse period of initiation(licensed but not initaiated?).Possibility of participation of DnaA in sugar metabolism for exemple by hyperstructure.
Dr masamichi kohiyama
Universite Paris deiderot; Inst.J.MOnod
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This page is a summary of: 60 Years of Studies into the Initiation of Chromosome Replication in Bacteria, Biomolecules, February 2025, MDPI AG,
DOI: 10.3390/biom15020203.
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