What is it about?
The 'SINGLE-MOLECULE DEMON' (single-molecule ratchet) in IMAGING/MICROSCOPY/SUPER-RESOLUTION MICROSCOPY (NANOSCOPY) and SPECTROSCOPY: Dilute Liquids and Live Cells https://www.growkudos.com/publications/10.2174%252F138920111795470949/reader
Why is it important?
Basic theory of anomalous subdiffusive motion: transport without significant hydrodynamic flow in live cells. The thermodynamic Single-Molecule DEMON: How to avoid him in the measurements of dilute liquids and live cells without immobilization or flow: https://www.linkedin.com/pulse/thermodynamic-single-molecule-demon-zeno-földes-papp/?trk=public_profile_article_view
Read the Original
This page is a summary of: Meaningful Interpretation of Subdiffusive Measurements in Living Cells (Crowded Environment) by Fluorescence Fluctuation Microscopy, Current Pharmaceutical Biotechnology, August 2010, Bentham Science Publishers, DOI: 10.2174/138920110791591454.
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Anomalous subdiffusive measurements by fluorescence correlations spectroscopy and simulations of translational diffusive behavior in live cells.
Trapping of molecules in live cells can exert at least two types of effects that could be important for function. First, live cells will have a strong influence on how far and how fast locally produced molecules will travel within the cytoplasm or the nucleoplasm. Over a time scale of 1 s, a molecule with an apparent diffusion coefficient Dapp of 2 μm2/ms, would diffuse about 60 μm. Thus, as long as molecules remained within the cytoplasm, their movement could be dominated by an anomalous diffusion process over distances. Second, anomalous diffusion reflects an increase in the spatial and temporal correlation of diffusing molecules which would be expected to promote activation of biochemical networks by intracellular signals trapped in live cells.
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