What is it about?
The natural laws of single-molecule detection in dilute liquids and live cells depends on the Stochastic Nature of Diffusion and was found by Zeno Földes-Papp for the first time. The meaningful time 'Tm' refers to the precision of a measurement in microscopy and nanoscopy (super-resolution microscopy) as wells as in spectroscopy with respect to the time of measuring just one and the same molecule in dilute solutions and live cells without immobilization or significant hydrodynamic flow. The advantages of the physical 'Theory of Single-Molecule Biophysics & Biochemistry Based On Individually Diffusing Molecules in Dilute Liquids and Live Cells' are evident: The 'SINGLE-MOLECULE DEMON' (single-molecule ratchet) in IMAGING/MICROSCOPY/SUPER-RESOLUTION MICROSCOPY (NANOSCOPY) and SPECTROSCOPY of Dilute Liquids and Live Cells that is The 'SINGLE-MOLECULE DEMON' (single-molecule ratchet) in Single-Molecule Localization Microscopy/Nanoscopy (Super-Resolution Microscopy)/Spectroscopy and Single-Molecule Laser Scanning Microscopy or Single-Molecule Image Analysis in liquids or live cells without immobilization on artificial or biological surfaces or without significant hydrodynamic flow • https://www.growkudos.com/publications/10.2174%252F138920111795470949/reader
Why is it important?
The FUNDAMENTAL NATURAL LAWS OF SINGLE-MOLECULE TIME RESOLUTION of freely diffusing molecules: The Single-Molecule Time-Resolution 'Tm' is the counterpart of the space-resolution in optical microscopy imposed by the Wave Nature of Light and first established by Ernst Abbe’s well-known formula in 1873 and later refined by Lord Rayleigh in 1896 to quantitate the measure of separation necessary between two Airy patterns in order to distinguish them as separate entities. Zeno. The thermodynamic Single-Molecule DEMON: How to avoid him in the measurements of dilute liquids and live cells without immobilization or flow:
Read the Original
This page is a summary of: Measurements of Single Molecules in Solution and Live Cells Over Longer Observation Times Than Those Currently Possible: The Meaningful Time, Current Pharmaceutical Biotechnology, April 2013, Bentham Science Publishers, DOI: 10.2174/1389201011314040009.
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Stochastic Nature of Diffusion: STOCHASTIC THERMODYNAMICS rules the physical formulation of the Single-Molecule Time-Resolution Tm
Original Research Article
What do Single-Molecule Localization or Single-Molecule Analysis in liquids or live cells without immobilization or hydrodynamic flow tell us about a single molecule?
Have a deeper look at the Theory of Single-Molecule Detection of one and the same molecule (the individual molecule) in dilute liquids and single live cells without significant hydrodynamic flow or immobilization on artificial or biological surfaces/membranes: The Single-Molecule Time Resolution in microsopy/nanoscopy (super-resolution microscopy)/ spectroscopy: The Földes-Papp's limits in measurement time to follow an individual molecule /individual particle (the same single molecule / the same single particle) with high probability. Sorry, the biggest breakthrough in microscopy/nanoscopy and spectroscopy would be a breakthrough in sensitivity for measuring an individual single molecule (one and the same molecule) over one, two or a couple of minutes without immobilization or significant hydrodynamic flow. Interested in joining this group? Single Molecule Biophysics & Biochemistry, see https://www.researchgate.net/profile/Zeno_Foeldes-Papp
Original Research Article
The thermodynamic Single-Molecule DEMON: How to avoid him in the measurements of dilute liquids and live cells without immobilization or flow: "Single-molecule time resolution in dilute liquids and live cells at the molecular scale: Constraints on the measurement time" in American Journal of Translational Medicine 2021, 5 (3), 154-165.
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