What is it about?
THE THERMODYNAMIC SINGLE-MOLECULE DEMON (Maxwell's Demon) IS IN YOUR SINGLE-MOLECULE EXPERIMENTS IN DILUTE LIQUDS AND LIVE CELLS WITHOUT IMMOBILIZATION OR HYDRODYNAMIC FLOW: How far apart do two molecules / two particles have to be in the time domain so that the required degree of separation between the two individual molecules / the two individual particles can be quantified at the molecular scale in order to distinguish them as separate entities without immobilization or hydrodynamic flow? Enjoy the real world of physics! Since mathematics provides a way to answer questions about the thermodynamic jitter in a clear, rational manner, with evidence to support it, mathematics is the reliable method necessary to get the best information on the movement of a single molecule / a single particle at the molecular scale in dilute liquids and live cells without immobilization or hydrodynamic flow.
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Why is it important?
It is the Theory of Single-Molecule Biophysics & Biochemistry Based On the Stochastic Nature of Diffusion. A theory has only the alternative of being right or wrong (Manfred Eigen, Nobel Prize winner). Földes-Papp's thermodynamically temporal single-molecule limits, or Földes-Papp's temporal resolution in diffraction limited and unlimited optical systems for dilute liquids or live cells without immobilization on a solid phase or membrane as well as without significant hydrodynamic flow,or even shorter, Földes-Papp's limits are the reference measures (so called 'golden standard') for any temporal resolution calculations/algorithms based upon parameter sets of (specific) measurement techniques, for example, photon counting statistics, Nyquist limit, FRET, etc., in order to ultimately justify whether or not a single molecule/particle (individual molecule/particle, selfsame molecule/particle) was measured during the observation/detection time in dilute liquids or live cells without immobilization on a solid phase or membrane as well as without significant hydrodynamic flow. Földes-Papp's limits are limits in measurement times that should not be exceeded when one wants to follow the selfsame molecule with high probability. Richard Levenson, Professor and Vice Chair for Strategic Technologies, Dept. at UC Davis, Davis, California, USA, wrote to me on May 24, 2021: ‘Much easier on Pluto, I imagine. A very cold, former planet. Not too much thermodynamic jitter at -233 C.’
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This page is a summary of: Getting high on single-molecule biophysics and biochemistry in dilute liquids and live cells without immobilization or significant hydrodynamic flow: the thermodynamic single-molecule demon, Current Pharmaceutical Biotechnology, June 2022, Bentham Science Publishers, DOI: 10.2174/1389201023666220616123928.
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NIH: National Library of Medicine, National Center of Biotechnology information. PubMed.
Gerd Baumann, Zeno Földes-Papp* (2022) Study on Single-Molecule Biophysics and Biochemistry in dilute liquids and live cells without immobilization or significant hydrodynamic flow: The thermodynamic Single-Molecule DEMON. Curr. Pharm. Biotechnol. 23: 14. 1750-1757. Published as 'Open Access' artiicle with colored Graphical Abstract by U.S. Bentham Science Publishers (USD 4, 205.00 paper funding): https://benthamscience.com/author-compliance/CPB
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