What is it about?
Annexin V forms trimeric structures which further assemble into two-dimensional crystal (2D crystal) lattices on negatively charged phospholipid bilayer in a Ca2+-dependent manner. We here show that the annexin V N-terminal has significant influence on 2D crystal formation using high-speed atomic force microscopy (HS-AFM) observations. We also present a quick purification method to purify recombinant annexin V without any residual affinity tag after protein purification.
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Why is it important?
We found that it would be better to use annexin V which does not contain any tag sequences such as His-tag or FLAG-tag at the N-terminus to prevent an experimental artifact not only in AFM observations but also other biological experiments.
Perspectives
Our results also indicate the important role of N-terminus regions in annexins to maintain functional diversity in the cell by altering the assembly state. In the future, it may be possible to artificially modify the functions of annexins by altering the N-terminus sequence.
Hiroki Konno
Kanazawa University
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This page is a summary of: Importance of annexin V N-terminus for 2D crystal formation and quick purification protocol of recombinant annexin V, PLOS One, December 2022, PLOS,
DOI: 10.1371/journal.pone.0278553.
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