Improved deconvolution of very weak confocal signals

What is it about?

We showed that when confocal image signals were very weak, the commercial Huygens image deconvolution software removed meaningful data. A workaround employing a standard image filter preserved the data. In collaboration with the company that makes Huygens, we subsequently confirmed that an updated version of the software fixed the problem.

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Why is it important?

Huygens is used routinely in many laboratories. By identifying and overcoming a limitation in the algorithm, we improved the sensitivity and reliability of image deconvolution for a large number of researchers.

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