What is it about?
Targeting induced local lesions in genomes (TILLING), developed as an alternative to cumbersome insertional mutagenesis, takes advantage of classical mutagenesis, sequence availability and high throughput screening for nucleotide polymorphisms in a targeted sequence. Since it is readily applicable to most plants, it remains a dominant non-genetic manipulation (GM) method for obtaining desirable mutations in known genes. In this chapter, we review here the discovery and technology with more focus on the ease of applications, Ecotilling, constraints, recent developments in polyploids crops, and the future of TILLING for crop breeding.
Why is it important?
The application of next-generation sequencing (NGS) has been a breakthrough change, which permits multiplexing of gene targets and genomes with TILLING as one of genome editing tool.
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This page is a summary of: Plant Biotechnology, Volume 1, December 2017, Taylor & Francis, DOI: 10.1201/9781315213743.
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