What is it about?
The letter deals with a technical problem relevant when the expression of human GITR is studied by flow cytometry
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Why is it important?
We demonstrate that the anti-GITR antibody clone 621, which is the most used to stain human GITR, does not bind GITR. For this reason, several studies conclude that human GITR is not expressed by human regulatory T cells (Treg). We suggest that studies evaluating GITR expression by flow cytometry must use an antibody different from 621 mAb, such as, for example, DT5D3 mAb.
Perspectives
Several studies on mouse models suggest that GITR is an important marker of Tregs. The result is confirmed in humans. However, several studies on humans using flow cytometry or citof demonstrate that GITR is not a marker of human Tregs. These results are due to the use of a mAb that does not bind GITR. Future studies must be performed with other anti-GITR mAbs.
Prof Giuseppe Nocentini
University of Perugia
Read the Original
This page is a summary of: Novel Immune Targets in Melanoma—Letter, Clinical Cancer Research, September 2019, American Association for Cancer Research (AACR),
DOI: 10.1158/1078-0432.ccr-19-1140.
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