Sex hormone dose escalation for treating abnormal sleep in ovariectomized rats: in vitro GABA synthesis in sleep-related brain areas

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The maximum tolerated dose coincided with the full dose of estradiol (17β-E2) and progesterone (P) used. Furthermore, the estrogen and progesterone combination treatments restored normal sleep in the menopause model compared to the SHAMs without toxic effects. The in vitro model demonstrated that 17β-E2 plus P presence in the assay increased the activity of GAD65 in the studied brain tissues. These results could be potentially important in laying the groundwork for developing a clinically applicable 17β-E2 plus P to improve the sleep of perimenopausal women with this problem. Therefore, it is necessary to carry out more studies to strengthen this line of research.

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