What is it about?
New method harnesses image scanning superresolution for enhanced photon efficiency in light-sheet microscopy
Featured Image
Photo by Clyde He on Unsplash
Why is it important?
In light-sheet fluorescence microscopy, the axial resolution and field of view are mutually constrained. Here we adopted a superresolution imaging technique, image scanning microscopy (ISM), to develop ISM-enhanced laterally swept light-sheet microscopy (iLSLM), which improves the optical sectioning and axial resolution without sacrificing photon efficiency.
Perspectives
We believe iLSLM will be suitable for applications where photobleaching is a severe problem, or the specimen is susceptible to phototoxicity. The current work of iLSLM is based on digital pixel reassignment, which significantly reduces the imaging speed. In the future, we will explore optical pixel reassignment to achieve the same imaging speed as ASLM.
Karl Zhanghao
Read the Original
This page is a summary of: Laterally swept light-sheet microscopy enhanced by pixel reassignment for photon-efficient volumetric imaging, Advanced Photonics Nexus, December 2022, SPIE,
DOI: 10.1117/1.apn.2.1.016001.
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