What is it about?

Plants host seizable population of bacteria on their roots(rhizosphere) and vegetative parts(phyllosphere). It is important to culture these plant- associated bacteria to study and boost their roles in supporting plant health and nutrition. Only a small fraction (less than 10 %) of such bacteria is culturable in laboratories. Therefore, we need to increase their culturability by various means. We successfully proved that it is a lot easier and more efficient to use plant-based, not chemically synthetic, culture media for their in vitro growth and in situ recovery.

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Why is it important?

The plant-based culture media in the form of tea- bagged plant powders are very practical and easy to apply. Compared to the generally used chemically-synthetic culture nedfua, the teabags culture media are nutritionally rich and provide diverse composite of natural nutrients to support bacteria very much affiliated to the tested host plant.

Perspectives

Goodby to the bottle(chemicals)-fed and welcome to the breast (plant)-fed rhizobacteria. Contrary to the use of chemically-synthetic culture media (with or without plant supplements), here we report for the first time on the sole use of plant -based culture media to grow and recover bacteria associated to plants. The proposed plant teabags by itself were sufficient and efficient to support wonderful growth of rhizobactetia. They resulted in very significant increases in culturability and rich diversity.

Prof Nabil A Hegazi
Cairo University

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This page is a summary of: Plant powder teabags: a novel and practical approach to resolve culturability and diversity of rhizobacteria, Physiologia Plantarum, June 2016, Wiley,
DOI: 10.1111/ppl.12469.
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