What is it about?
The paper reports universal primers that amplify a nuclear intron a wide variety of decapod crustaceans.
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Why is it important?
Publishing genetic studies based entirely on mitochondrial DNA is becoming difficult. A single intron can help to explore issues such as reproductive isolation and sex-biased dispersal.
Perspectives
The primers described here have been a simple but efficient way to explore genetic structure, gene flow, reproductive isolation and mitonuclear discordance. Using introns is often a cost-effective alternative to developing microsatellite markers.
Prof. Peter R Teske
University of Johannesburg
Read the Original
This page is a summary of: Intron-spanning primers for the amplification of the nuclear ANT gene in decapod crustaceans, Molecular Ecology Resources, May 2009, Wiley,
DOI: 10.1111/j.1755-0998.2009.02534.x.
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