Evaluation of aging, diabetes mellitus, and skin wounds by scanning acoustic microscopy with protease digestion

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I hope the method using scanning acoustic microscope will become familiar to other scientists. This microscope provide not only fine histology but also mechanical properties of the tissue. Its advantage over light microscopy are as follows: 1.No staining is necessary. 2.It takes a few minutes to obtain histology images. 3.Obtained images are so originally digital that statistical analysis is easy. 4.Repeated observations after operating the section are possible such as protease digestion. Because higher resolution is available as sound frequency increases, observation of cytoplasmic organella and intranuclear structure is possible experimentally.

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