What is it about?
A numerical scoring system is presented, for evaluating fixation of rodent brain and kidney for subsequent light microscopy of paraffin sections. Results are presented for 15 fixative mixtures, and discussed in relation to earlier published comparisons.
Why is it important?
Other investigators, notably J.R. Baker, have attributed "grades" from I to V to various fixatives, but without exactly defining the criteria of structural preservation for the different grades. With the new numerical system, the criteria to look for in the sections are defined and illustrated. Brain and kidney are two tissues in which fixation artifacts are commonly seen and easy to evaluate.
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This page is a summary of: A system for quantitative evaluation of fixatives for light microscopy using paraffin sections of kidney and brain, Biotechnic & Histochemistry, January 2009, Taylor & Francis,
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