What is it about?

A well known example of an indigogenic substrate is 5-chloro-6-bromoindoxyl-ß-D-galactoside (X-gal), which gives insoluble blue deposits in cells expressing the lac-z reporter gene. This review article covers many substrates that generate coloured indigoid dyes when acted upon by enzymes. The substrates are used in biochemistry, histochemistry and bacteriology.

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Why is it important?

This review article provides references to original literature from the introduction of the first indigogenic substrate, 5-bromoindoxyl acetate, by S.J.Holt in 1952 until 2006.

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This page is a summary of: Indigogenic substrates for detection and localization of enzymes, Biotechnic & Histochemistry, January 2007, Taylor & Francis,
DOI: 10.1080/10520290701375278.
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