What is it about?
A well known example of an indigogenic substrate is 5-chloro-6-bromoindoxyl-ß-D-galactoside (X-gal), which gives insoluble blue deposits in cells expressing the lac-z reporter gene. This review article covers many substrates that generate coloured indigoid dyes when acted upon by enzymes. The substrates are used in biochemistry, histochemistry and bacteriology.
Why is it important?
This review article provides references to original literature from the introduction of the first indigogenic substrate, 5-bromoindoxyl acetate, by S.J.Holt in 1952 until 2006.
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This page is a summary of: Indigogenic substrates for detection and localization of enzymes, Biotechnic & Histochemistry, January 2007, Taylor & Francis,
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