Computation and conversion of brain pH values obtained with two algorithms of phosphorus magnetic resonance spectroscopy data analysis

What is it about?

The intracellular brain pH in phosphoric magnetic resonance spectroscopy is calculated using the chemical shift of the inorganic phosphate to phosphocreatine with the Henderson-Hasselbalch equations. Researchers use various mathematical formulas that have different parameters. The pH values were calculated using the two most commonly used algorithms for the theoretical chemical shift values between inorganic phosphate and phosphocreatine in the brain. The relationships between pH results and the applied mathematical formula were determined. The pH results were compared with each other using the appropriate t-tests. Mathematical formulas were designed to simplify the conversion of pH values obtained by two methods of data analysis. The pH values with were obtained this way did not differ significantly from the pH values calculated directly from the given formula. The computed mathematical formulas will make it possible to simplify pH calculations and conversions without knowing the chemical shift between inorganic phosphate and phosphocreatine but only basing on the known pH values obtained by one of the formulas.

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