What is it about?
To create this technology we built a high-throughput CRISPR pipeline and synthetically engineered hundreds of yeast strains for studying human G protein-coupled receptors (GPCRs). We then demonstrated how this technology can be used to rapidly identify new ligands and drugs for GPCRs by screening a library of human metabolites.
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Why is it important?
This technology is important because it enable many different GPCRs to pooled together in a single quick, easy, and inexpensive experiment. As such, we can evaluate the specificity of a given ligand are drug against hundreds of GPCRs in a single test tube. This had never been done before.
Perspectives
Because ligands are tested against many receptors simultaneously, this new GPCR profiling technology will eventually enable the physical screening of huge numbers of compounds, bringing wet-laboratory experimental throughput a step closer to what is done using computational screening methods.
Daniel Isom
University of Miami Miller School of Medicine
Read the Original
This page is a summary of: DCyFIR: a high-throughput CRISPR platform for multiplexed G protein-coupled receptor profiling and ligand discovery, Proceedings of the National Academy of Sciences, May 2020, Proceedings of the National Academy of Sciences,
DOI: 10.1073/pnas.2000430117.
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