What is it about?
We present a method to recapitulate endochondral bone formation, the process through which long bones develop and grow, in cell culture. Our method begins with induced pluripotent stem cells (iPSCs) and produces uniform cartilage that progressively matures, mirroring development. Hypertrophic chondrocytes can transition to osteoblasts in our culture system, and we provide detailed analysis of the gene expression profile and changes during maturation to support this claim.
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Why is it important?
Our method can be used to interrogate cartilage differentiation and development, including chondrocyte transition to osteoblasts, model genetic cartilage and bone disorders and search for therapies.
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This page is a summary of: Modeling human skeletal development using human pluripotent stem cells, Proceedings of the National Academy of Sciences, May 2023, Proceedings of the National Academy of Sciences, DOI: 10.1073/pnas.2211510120.
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