What is it about?

This works demonstrates that bacteria alter the position and action of transcription factors in the cell in response to environmental nutrients by post-transcriptional modifications that utilize metabolic intermediates such as acetate and succinate..

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Why is it important?

This is a novel mechanism by which the action of transcription factors in general and the cAMP receptor protein (CRP), in particular, is modified by nutrient availability. It also uncovers a novel interaction between CRP and the dual function peptidase Peptidase A (PepA) and establishes PepA as a global transcription factor in its own right.


The transcription factor CRP has been studied for decades but no one ever reported on its position in the cell. We happened upon its membrane association by accident when we tested it as a cytoplasmic control for cellular fractionations. In fact, conditional cell membrane association may play a bigger role than previously appreciated in regulating protein function.

Paula Watnick
Boston Children's Hospital

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This page is a summary of: Sequestration of a dual function DNA-binding protein by Vibrio cholerae CRP, Proceedings of the National Academy of Sciences, November 2022, Proceedings of the National Academy of Sciences,
DOI: 10.1073/pnas.2210115119.
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