What is it about?
Cyanobacteria are an ancient group of photosynthetic microbes that occur in the ocean and most inland waters. They are keystone organisms in global carbon fixation. Their great carbon-assimilation capability arises from a specialized virus-like protein organelle, the carboxysome, which comprises hundreds of proteins that form a shell to encapsulate the CO2-fixing enzymes Rubisco and carbonic anhydrase.
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Why is it important?
It is a mystery how cyanobacterial cells generate the complex carboxysome structure and pack Rubisco enzymes in the organelle to have biological functions. Here we explore the significance of one assembly factor, Raf1, in Rubisco assembly and carboxysome formation. We show that Raf1 mediates Rubisco assembly; without Raf1, carboxysome proteins are prone to form intermediate assemblies and small carboxysome-like structures rather than intact carboxysomes. Our results suggest a model of the Raf1-mediated biogenesis of carboxysomes and provide advanced knowledge of carboxysome assembly and function.
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This page is a summary of: Rubisco accumulation factor 1 (Raf1) plays essential roles in mediating Rubisco assembly and carboxysome biogenesis, Proceedings of the National Academy of Sciences, July 2020, Proceedings of the National Academy of Sciences, DOI: 10.1073/pnas.2007990117.
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