What is it about?
The work describes ways how to pattern light in the focal space of a multi-photon imaging system through acousto-optic spatial light modulation. To this end, we describe numerical algorithms to derive suitable acousto-optic holograms from wanted focal light patterns.
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Why is it important?
Fast switching between patterns of structured focal light is essential to a recent method of functional neuro-imaging named "3D Custom-Access Serial Holography" (3D-CASH) (Nat Meth 19(1) doi: 10.1038/s41592-021-01329-7). In 3D-CASH, a laser beam scans a 3D neural circuit of interconnected neurons by random-access to trace the information transfer in distributed neuronal networks at high temporal resolution via detection of the fluorescence signal emitted from molecular activity indicators in neurons targeted by the laser beam.
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This page is a summary of: Acousto-optic holography for pseudo-two-dimensional dynamic light patterning, APL Photonics, April 2024, American Institute of Physics,
DOI: 10.1063/5.0185857.
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