What is it about?
Single-molecule experiments detecting Förster Resonance Energy Transfer (FRET) offer nanosecond time resolution to study the motions biomolecules in a structural context. We present parametric relations (FRET-lines) between two spectroscopic FRET observables, that facilitate a graphical interpretation of experimental data. Model-based FRET-lines serve as pathfinders to decode the dynamic fingerprint revealing the mechanism of the underlying molecular dynamics at a glance.
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Why is it important?
Conformational dynamics of biomolecules are of fundamental importance for their function.
Perspectives
Model-based FRET-lines serve as pathfinders to decode the dynamic fingerprint of biomolecules from spectroscopic signatures by eye. FRET-lines allow one to determine the number, location and shape of molecular populations in single-molecule burst frequency histograms, revealing the mechanism of the underlying molecular dynamics at a glance.
Claus Seidel
Heinrich-Heine-Universitat Dusseldorf
Read the Original
This page is a summary of: Unraveling multi-state molecular dynamics in single-molecule FRET experiments. I. Theory of FRET-lines, The Journal of Chemical Physics, April 2022, American Institute of Physics,
DOI: 10.1063/5.0089134.
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