What is it about?

We present single-shot quantitative phase imaging with polarization differential interference contrast (PDIC) which enables a conventional differential interference contrast microscope to perform real-time high-resolution quantitative phase imaging of cells and tissue sections label-free at the camera frame rate.

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Why is it important?

(1) PDIC converts a conventional DIC microscope to a powerful single-shot quantitative phase imaging (QPI) approach of high spatial resolution. (2) An algorithm with total variation regularization is introduced to solve the quantitative phase map from the partial derivative along one single axis, improving accuracy and image quality compared to conventional approaches.


PDIC integrates simply with epifluorescence imaging for coregistered simultaneous measurements. PDIC may emerge as an attractive quantitative phase imaging method for biology and medicine.

Min Xu
Hunter College

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This page is a summary of: Single-shot quantitative phase imaging with polarization differential interference contrast, Applied Physics Letters, October 2021, American Institute of Physics,
DOI: 10.1063/5.0065129.
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