Design an Orderly Logic Gate to Construct Plasmids in GenoCAD

What is it about?

Rapid developments have been made in synthetic biology within the past two decades, particularly in combination with chemistry, computer science, and other disciplines. Genetic components and internal features have been a main focus of research for synthetic biologists. Logic gates can be applied in various disciplines, but have not yet been used for plasmid design. GenoCAD is a computer-aided design software program for synthetic biology that can be used to design complex structures. Thus, in this study, we analysed a large, commonly used data set containing over 70,000 feature sequences and eventually obtained comprehensive information for a complete data set without redundancy. By analysing the internal feature sequences, we input the most representative data in the GenoCAD platform, along with design rules and grammar for constructing high-quality practical parts. Additionally, the orderly logic gate for building biological parts designed in this study may be useful for professionals and nonprofessionals and may have applications in the design of a new biological computer. Finally, we compared the constructed plasmid with other successful examples in BLAST and PlasMapper software to demonstrate the rationality of the orderly logic gate.

Featured Image

Why is it important?

The orderly logic gate presented in this paper can be used conveniently and easily in plasmid design. This logic gate may also have independent uses and could be incorporated into other software. In future studies, we will focus on analysis of the internal characteristics of parts in order to improve the features of these parts. We will then carry out laboratory ex-periments to support the findings and develop a new biological computer.